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The map label for this gene is potB [H]

Identifier: 119386173

GI number: 119386173

Start: 604937

End: 606229

Strand: Direct

Name: potB [H]

Synonym: Pden_3461

Alternate gene names: 119386173

Gene position: 604937-606229 (Clockwise)

Preceding gene: 119386172

Following gene: 119386174

Centisome position: 34.97

GC content: 67.52

Gene sequence:

>1293_bases
ATGGCAGACACCTCGGCGAATGGCGTGAGCTTCGCGCCCGCGCCCGCTCACCGGACCGGGCTTGTGACCGCCGACGGTCG
TCCGTTGAGATCTGCGCTTGCGCGGAGTTCGCGTCGTGCGCGTCGTCGTGCGTTTTTCCTGGTGCTTCCGCTTCTGCTTT
TCGTGCTGATCACCTTCGTGGTGCCGATCGGGCAGATGCTGCAGCGCTCGGTCAAGAACGAGGGCTTCTCGGCCAACATG
CCGCAGCTCAGCGCCTGGTTCCACGACAACCCCCGCGGCACCGAGCCCGACGATGCGGCCTGGGCGGCGCTGGCCGCCGA
CCTGACCGCCGCCGCCCAGGCCCGCACCATCGGCGTGGTCGGCACCCGCATCAACTACGACATGCCGGGCACGCGGTCGC
TGTTCACCTCGGCCGGGCGGCAGGCGCGCGGCGGCATCGAGCCGCCCTATCGCGAGGCGATCCTGGAGATGGACGCCCGG
TGGGGCAATCCCCGGCTGTGGTCGGTGATGCGAGAGGCCGCCTCGCCCTATACCGCCAACTTCTACCTCGCGGCGGTGGA
CCGCACCCGCGACGACCAGGGCGACATCGTCTCCGCCCCGGCGCAGCAGCAGATCTACGGCAAGCTGTTCCTGCGCACCT
TCTGGCTGTCGCTGGCCATCACCGCCACCACCTTCCTCCTGGGCTTTCCGGTCGCGCATCTGCTGGCCACCCTGCCGATG
CGGCAGTCCAACCTGCTGATGATCCTGGTGCTGCTGCCGTTCTGGACCTCGCTGCTGGTCAGGACCACGGCCTGGATCGT
GCTGTTGCAGCAGCAGGGCGTGGTCAACGACGTGCTGGTCTGGCTGGGGGTGATCGGCAACAACCAGCGGCTGCAGATGA
TCTTCAACCAGACCGGCACGATCATCGCCATGACCCATATCCTGCTGCCCTTCATGATCCTGCCGCTCTATTCGGTGATG
CGCACCATCAATCCCAGCTACGTGCGCGCCGCGCGCAGCCTGGGCGCGACCAGCTGGACCGCCTTCCGCCGCATCTATTT
CCCGCAGACCCTGCCGGGGCTGGGGGCGGGGGCGCTGCTGGTGTTCATCCTGGCGGTGGGCTACTACATCACGCCGGCGC
TGGTCGGCGGCTCGTCGGGGCAGCTGATCTCCAACATGATCGCGATGCACATGACGCAGACGCTGAACTGGTCCATGGCG
GCCGCGCTGGCGGCGCTGCTGCTGGGCGGGGTGCTGATCCTCTACTGGGTCTACGACCGTCTCGTCGGCATCGACAACCT
GAAACTGGGGTAA

Upstream 100 bases:

>100_bases
CTGACGGGCTGGGGCGCGGGATGCGGCAATCCACGAAACGAGCCGCGGTGCCGCCTTGGCGCCGCAGCCTTCGAACCGAA
CCGAACCGGAGACAGAGACC

Downstream 100 bases:

>100_bases
CCGCCATGGCGCTTCCGACCTATGCCTCGCCCTTGGAGAAGACCTGGCACTACGCCTATCTGGCGATCTGCGCGGCGATC
TTCTTCTTCCTGATCGCGCC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MADTSANGVSFAPAPAHRTGLVTADGRPLRSALARSSRRARRRAFFLVLPLLLFVLITFVVPIGQMLQRSVKNEGFSANM
PQLSAWFHDNPRGTEPDDAAWAALAADLTAAAQARTIGVVGTRINYDMPGTRSLFTSAGRQARGGIEPPYREAILEMDAR
WGNPRLWSVMREAASPYTANFYLAAVDRTRDDQGDIVSAPAQQQIYGKLFLRTFWLSLAITATTFLLGFPVAHLLATLPM
RQSNLLMILVLLPFWTSLLVRTTAWIVLLQQQGVVNDVLVWLGVIGNNQRLQMIFNQTGTIIAMTHILLPFMILPLYSVM
RTINPSYVRAARSLGATSWTAFRRIYFPQTLPGLGAGALLVFILAVGYYITPALVGGSSGQLISNMIAMHMTQTLNWSMA
AALAALLLGGVLILYWVYDRLVGIDNLKLG

Sequences:

>Translated_430_residues
MADTSANGVSFAPAPAHRTGLVTADGRPLRSALARSSRRARRRAFFLVLPLLLFVLITFVVPIGQMLQRSVKNEGFSANM
PQLSAWFHDNPRGTEPDDAAWAALAADLTAAAQARTIGVVGTRINYDMPGTRSLFTSAGRQARGGIEPPYREAILEMDAR
WGNPRLWSVMREAASPYTANFYLAAVDRTRDDQGDIVSAPAQQQIYGKLFLRTFWLSLAITATTFLLGFPVAHLLATLPM
RQSNLLMILVLLPFWTSLLVRTTAWIVLLQQQGVVNDVLVWLGVIGNNQRLQMIFNQTGTIIAMTHILLPFMILPLYSVM
RTINPSYVRAARSLGATSWTAFRRIYFPQTLPGLGAGALLVFILAVGYYITPALVGGSSGQLISNMIAMHMTQTLNWSMA
AALAALLLGGVLILYWVYDRLVGIDNLKLG
>Mature_429_residues
ADTSANGVSFAPAPAHRTGLVTADGRPLRSALARSSRRARRRAFFLVLPLLLFVLITFVVPIGQMLQRSVKNEGFSANMP
QLSAWFHDNPRGTEPDDAAWAALAADLTAAAQARTIGVVGTRINYDMPGTRSLFTSAGRQARGGIEPPYREAILEMDARW
GNPRLWSVMREAASPYTANFYLAAVDRTRDDQGDIVSAPAQQQIYGKLFLRTFWLSLAITATTFLLGFPVAHLLATLPMR
QSNLLMILVLLPFWTSLLVRTTAWIVLLQQQGVVNDVLVWLGVIGNNQRLQMIFNQTGTIIAMTHILLPFMILPLYSVMR
TINPSYVRAARSLGATSWTAFRRIYFPQTLPGLGAGALLVFILAVGYYITPALVGGSSGQLISNMIAMHMTQTLNWSMAA
ALAALLLGGVLILYWVYDRLVGIDNLKLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=222, Percent_Identity=36.036036036036, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1787080, Length=204, Percent_Identity=34.8039215686275, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787713, Length=209, Percent_Identity=32.0574162679426, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 47193; Mature: 47062

Theoretical pI: Translated: 10.93; Mature: 10.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTSANGVSFAPAPAHRTGLVTADGRPLRSALARSSRRARRRAFFLVLPLLLFVLITFV
CCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPIGQMLQRSVKNEGFSANMPQLSAWFHDNPRGTEPDDAAWAALAADLTAAAQARTIGVV
HHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
GTRINYDMPGTRSLFTSAGRQARGGIEPPYREAILEMDARWGNPRLWSVMREAASPYTAN
EEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCE
FYLAAVDRTRDDQGDIVSAPAQQQIYGKLFLRTFWLSLAITATTFLLGFPVAHLLATLPM
EEEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RQSNLLMILVLLPFWTSLLVRTTAWIVLLQQQGVVNDVLVWLGVIGNNQRLQMIFNQTGT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
IIAMTHILLPFMILPLYSVMRTINPSYVRAARSLGATSWTAFRRIYFPQTLPGLGAGALL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHH
VFILAVGYYITPALVGGSSGQLISNMIAMHMTQTLNWSMAAALAALLLGGVLILYWVYDR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVGIDNLKLG
HHCCCCCCCC
>Mature Secondary Structure 
ADTSANGVSFAPAPAHRTGLVTADGRPLRSALARSSRRARRRAFFLVLPLLLFVLITFV
CCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPIGQMLQRSVKNEGFSANMPQLSAWFHDNPRGTEPDDAAWAALAADLTAAAQARTIGVV
HHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
GTRINYDMPGTRSLFTSAGRQARGGIEPPYREAILEMDARWGNPRLWSVMREAASPYTAN
EEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCE
FYLAAVDRTRDDQGDIVSAPAQQQIYGKLFLRTFWLSLAITATTFLLGFPVAHLLATLPM
EEEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RQSNLLMILVLLPFWTSLLVRTTAWIVLLQQQGVVNDVLVWLGVIGNNQRLQMIFNQTGT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
IIAMTHILLPFMILPLYSVMRTINPSYVRAARSLGATSWTAFRRIYFPQTLPGLGAGALL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHH
VFILAVGYYITPALVGGSSGQLISNMIAMHMTQTLNWSMAAALAALLLGGVLILYWVYDR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVGIDNLKLG
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]