Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 119386159

GI number: 119386159

Start: 588344

End: 589516

Strand: Direct

Name: mexA [H]

Synonym: Pden_3447

Alternate gene names: 119386159

Gene position: 588344-589516 (Clockwise)

Preceding gene: 119386158

Following gene: 119386160

Centisome position: 34.01

GC content: 70.67

Gene sequence:

>1173_bases
ATGACCTTTCGCTTTCTCCATCGGCTTGCCCGGCTGGGCATTGCCCTGGCCGCTTTTTCCCTTGCGGGACAGGCCGCGGC
GCAGCAGCCCGCCGCGCCCGGTCCCAGGCAGGTCGGGGTGATTTCCGTCACGGCCGAATCCGTGCCGCTGGTCAGCACGC
TGCCCGGTCGCGCCGTGGCACGGGATGCGGTGGACATCCGCCCGCGCGTGGACGGTTTCGTGACCGAGGTGCTCTACAAT
CCCGGCCAGCCGATCAAGGTGGGCGATCCCATGTTCCGCATCGATCCGACCACCTATGAGGCCGCGGTCCAGGAGGCGCG
GGCCAACCTTGCCTCGGCCAAGGCGGCCGTGCCGCAGGCCGAGGCGGCCTATGAGCGGTCGCGCCGGCTGCAGGGCTCGG
GCTCGACCCAGGCGGCCCTGGAACAGGCGCAGGCCACGATGGAGCAGGCGCAGGCCGCCGCCGCCGCCGCCGAGGCGGCG
CTGAAGCTGGCCGAGACGCAGCTGTCCTGGACCACGATCGCCAGCCCGCTGGACGGGCTGCCCTCGGTCGCCGCGGTCTC
GCCGGGCGACCTGGTCACCGCGGGGCAAAGCGATGCGCTGGCGACGGTCACCCAGCTCGATCCCATCGACGTGGACATGT
ACGAACCCTCGGCCCGGCTGCAACGCATCCGCGAGCGCATCGAGTCGGGCCAGATCAGGATGAGCCAGGGGCTGAAGGCG
CAGCTGACGCTGGAGAACGGCGTGACCTATGCCGCCACGGGCGAGATGGTGGCGCCGGGCTATACCGTCTCGACCTCGAC
CGGGGCCATCGACTTCCGTTTCCGCTTCGACAATCCCGAGCGCCGCATCCTGCCCGGCATGTTCGTGCGCGGCCGGATCG
AGATCGGCCGGATCCAGGCGGTGCTGGTGCCGCAGATGGCTGCGACGCGCGGGCGCGACGGCACGCTTTCGGCCTGGGTG
GCCGAGGATGGCAAGGCGGTAAAGCGCGTGCTGACCGAGGAGGGCGTGCATGAGAACAGCTGGATCGTCACCGACGGGCT
GAAGGACGGCGACGCGCTGATCGTGAACGGTGGCACCAATCTGGCCGAAGGCGCCGAGGTCGCGCCGGTTGCGGTCGAGA
TCGACGAGAACGGCGTGGTCCGCGACCTGCCGGCGACGCAGCAGGCGGAGTGA

Upstream 100 bases:

>100_bases
ACGGCACGCAGGACCGAAGATGAGGCCGATCCCTCCCGCCGATGCGCGGCCTGTCGCAGGCTGAACCGCACCAACCCGTT
TTCGACCAAGGATGATCCGC

Downstream 100 bases:

>100_bases
GCCGATGGCCCGTTTCTTCATTCACCGCCCCGTCTTCGCTTGGGTTCTGGCCATCATCACCATGCTGGCCGGGGTCTATG
GCCTGTCCAGCCTGCCCATC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTFRFLHRLARLGIALAAFSLAGQAAAQQPAAPGPRQVGVISVTAESVPLVSTLPGRAVARDAVDIRPRVDGFVTEVLYN
PGQPIKVGDPMFRIDPTTYEAAVQEARANLASAKAAVPQAEAAYERSRRLQGSGSTQAALEQAQATMEQAQAAAAAAEAA
LKLAETQLSWTTIASPLDGLPSVAAVSPGDLVTAGQSDALATVTQLDPIDVDMYEPSARLQRIRERIESGQIRMSQGLKA
QLTLENGVTYAATGEMVAPGYTVSTSTGAIDFRFRFDNPERRILPGMFVRGRIEIGRIQAVLVPQMAATRGRDGTLSAWV
AEDGKAVKRVLTEEGVHENSWIVTDGLKDGDALIVNGGTNLAEGAEVAPVAVEIDENGVVRDLPATQQAE

Sequences:

>Translated_390_residues
MTFRFLHRLARLGIALAAFSLAGQAAAQQPAAPGPRQVGVISVTAESVPLVSTLPGRAVARDAVDIRPRVDGFVTEVLYN
PGQPIKVGDPMFRIDPTTYEAAVQEARANLASAKAAVPQAEAAYERSRRLQGSGSTQAALEQAQATMEQAQAAAAAAEAA
LKLAETQLSWTTIASPLDGLPSVAAVSPGDLVTAGQSDALATVTQLDPIDVDMYEPSARLQRIRERIESGQIRMSQGLKA
QLTLENGVTYAATGEMVAPGYTVSTSTGAIDFRFRFDNPERRILPGMFVRGRIEIGRIQAVLVPQMAATRGRDGTLSAWV
AEDGKAVKRVLTEEGVHENSWIVTDGLKDGDALIVNGGTNLAEGAEVAPVAVEIDENGVVRDLPATQQAE
>Mature_389_residues
TFRFLHRLARLGIALAAFSLAGQAAAQQPAAPGPRQVGVISVTAESVPLVSTLPGRAVARDAVDIRPRVDGFVTEVLYNP
GQPIKVGDPMFRIDPTTYEAAVQEARANLASAKAAVPQAEAAYERSRRLQGSGSTQAALEQAQATMEQAQAAAAAAEAAL
KLAETQLSWTTIASPLDGLPSVAAVSPGDLVTAGQSDALATVTQLDPIDVDMYEPSARLQRIRERIESGQIRMSQGLKAQ
LTLENGVTYAATGEMVAPGYTVSTSTGAIDFRFRFDNPERRILPGMFVRGRIEIGRIQAVLVPQMAATRGRDGTLSAWVA
EDGKAVKRVLTEEGVHENSWIVTDGLKDGDALIVNGGTNLAEGAEVAPVAVEIDENGVVRDLPATQQAE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1786668, Length=360, Percent_Identity=35, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789665, Length=349, Percent_Identity=34.3839541547278, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082036, Length=328, Percent_Identity=26.219512195122, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789637, Length=244, Percent_Identity=27.8688524590164, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081951, Length=162, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41174; Mature: 41043

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRFLHRLARLGIALAAFSLAGQAAAQQPAAPGPRQVGVISVTAESVPLVSTLPGRAVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHH
RDAVDIRPRVDGFVTEVLYNPGQPIKVGDPMFRIDPTTYEAAVQEARANLASAKAAVPQA
CCCCCCCCCHHHHHHHHHHCCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCH
EAAYERSRRLQGSGSTQAALEQAQATMEQAQAAAAAAEAALKLAETQLSWTTIASPLDGL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
PSVAAVSPGDLVTAGQSDALATVTQLDPIDVDMYEPSARLQRIRERIESGQIRMSQGLKA
CCCEECCCCCEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCEE
QLTLENGVTYAATGEMVAPGYTVSTSTGAIDFRFRFDNPERRILPGMFVRGRIEIGRIQA
EEEECCCEEEEECCCEECCCEEEECCCCEEEEEEEECCCCCCCCCCEEEECEEEECEEEE
VLVPQMAATRGRDGTLSAWVAEDGKAVKRVLTEEGVHENSWIVTDGLKDGDALIVNGGTN
EEECHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCCC
LAEGAEVAPVAVEIDENGVVRDLPATQQAE
CCCCCCCCEEEEEECCCCCEECCCCCCCCC
>Mature Secondary Structure 
TFRFLHRLARLGIALAAFSLAGQAAAQQPAAPGPRQVGVISVTAESVPLVSTLPGRAVA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEECCCCCHHH
RDAVDIRPRVDGFVTEVLYNPGQPIKVGDPMFRIDPTTYEAAVQEARANLASAKAAVPQA
CCCCCCCCCHHHHHHHHHHCCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCH
EAAYERSRRLQGSGSTQAALEQAQATMEQAQAAAAAAEAALKLAETQLSWTTIASPLDGL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
PSVAAVSPGDLVTAGQSDALATVTQLDPIDVDMYEPSARLQRIRERIESGQIRMSQGLKA
CCCEECCCCCEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCEE
QLTLENGVTYAATGEMVAPGYTVSTSTGAIDFRFRFDNPERRILPGMFVRGRIEIGRIQA
EEEECCCEEEEECCCEECCCEEEECCCCEEEEEEEECCCCCCCCCCEEEECEEEECEEEE
VLVPQMAATRGRDGTLSAWVAEDGKAVKRVLTEEGVHENSWIVTDGLKDGDALIVNGGTN
EEECHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCCC
LAEGAEVAPVAVEIDENGVVRDLPATQQAE
CCCCCCCCEEEEEECCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]