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The map label for this gene is trmB

Identifier: 119357386

GI number: 119357386

Start: 1799210

End: 1799824

Strand: Direct

Name: trmB

Synonym: Cpha266_1584

Alternate gene names: 119357386

Gene position: 1799210-1799824 (Clockwise)

Preceding gene: 119357385

Following gene: 119357387

Centisome position: 57.41

GC content: 47.97

Gene sequence:

>615_bases
ATGTTCATGGATAGCAGAGAAACATTGTGCGAGAACCCCGTCATTATCACAGACCGGGATGCATGGAAGGAGATAAATCT
GAAGGATGCCGGAACGATCGAAGTTGAAATAGGCTTCGGCAGCGGCGAGTATCTTCTCAGAAGAGCATCGGAATACCCTG
AAAGACTGTTTATCGGCATCGAAAAGAAACCCGGAATGATAACGGAGGTTTCAAAAAGAGTTGCAAGCCATAACCTCAAT
AATATCCGGTTGCTCGAGTCGTGCGCCAAAGACGCCTTCGCCGACCTCTTCCCTGCAAATTCGATTTCACGTGTCTATTC
GCTCTTTCCCGACCCCTGGCCAAAAAGAAAACATCTCCACTACAGACTCTTTTCATCGGAATACCTGCGACTGCTCAATA
ACCGGCTTATTTTCGGCTCCGAGGCGCTCATCGTAACCGACAGCGAAGATTACTGCAACTGGATGCTGAAACAGCTACCC
GATACCGGCTTCGAGGTTGAAAACAGCATCATACCACCGCAATTCGATACCAGATTCGAACGGAAATGGCTGGAGCAGAA
CTTCAACACATTCTTCAGAATTCTGCTCAAAAAAGCACGGCACATCGAAACCTGA

Upstream 100 bases:

>100_bases
CCGGCTTACGAAAAGGATTGACAAAATGATAATGAAAGGCATTCTCACCAAGAATATCCCTTCGTCAAACTGAAGCCGAC
GTCACCCGTTATTCCACTTT

Downstream 100 bases:

>100_bases
AAGCTATCGGCAGTTCCCTTCCCCCGGATTATCTGCCGCTATGTAGAGTAAAATTAAACCATCAGGGACAATTTTCAAGT
CCGAACTCCCTTGCAGACGC

Product: putative methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MFMDSRETLCENPVIITDRDAWKEINLKDAGTIEVEIGFGSGEYLLRRASEYPERLFIGIEKKPGMITEVSKRVASHNLN
NIRLLESCAKDAFADLFPANSISRVYSLFPDPWPKRKHLHYRLFSSEYLRLLNNRLIFGSEALIVTDSEDYCNWMLKQLP
DTGFEVENSIIPPQFDTRFERKWLEQNFNTFFRILLKKARHIET

Sequences:

>Translated_204_residues
MFMDSRETLCENPVIITDRDAWKEINLKDAGTIEVEIGFGSGEYLLRRASEYPERLFIGIEKKPGMITEVSKRVASHNLN
NIRLLESCAKDAFADLFPANSISRVYSLFPDPWPKRKHLHYRLFSSEYLRLLNNRLIFGSEALIVTDSEDYCNWMLKQLP
DTGFEVENSIIPPQFDTRFERKWLEQNFNTFFRILLKKARHIET
>Mature_204_residues
MFMDSRETLCENPVIITDRDAWKEINLKDAGTIEVEIGFGSGEYLLRRASEYPERLFIGIEKKPGMITEVSKRVASHNLN
NIRLLESCAKDAFADLFPANSISRVYSLFPDPWPKRKHLHYRLFSSEYLRLLNNRLIFGSEALIVTDSEDYCNWMLKQLP
DTGFEVENSIIPPQFDTRFERKWLEQNFNTFFRILLKKARHIET

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=127, Percent_Identity=30.7086614173228, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CHLPD (A1BGS9)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912030.1
- ProteinModelPortal:   A1BGS9
- SMR:   A1BGS9
- STRING:   A1BGS9
- GeneID:   4569766
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1584
- NMPDR:   fig|290317.7.peg.1667
- eggNOG:   COG0220
- HOGENOM:   HBG405263
- OMA:   PDPWWKR
- ProtClustDB:   CLSK2320740
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 23957; Mature: 23957

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 36-36 BINDING 61-61 BINDING 111-111 BINDING 115-115 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMDSRETLCENPVIITDRDAWKEINLKDAGTIEVEIGFGSGEYLLRRASEYPERLFIGI
CCCCCCHHHHCCCEEEECCCCHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEE
EKKPGMITEVSKRVASHNLNNIRLLESCAKDAFADLFPANSISRVYSLFPDPWPKRKHLH
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCE
YRLFSSEYLRLLNNRLIFGSEALIVTDSEDYCNWMLKQLPDTGFEVENSIIPPQFDTRFE
EEHHHHHHHHHHHCCEEECCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
RKWLEQNFNTFFRILLKKARHIET
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFMDSRETLCENPVIITDRDAWKEINLKDAGTIEVEIGFGSGEYLLRRASEYPERLFIGI
CCCCCCHHHHCCCEEEECCCCHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEE
EKKPGMITEVSKRVASHNLNNIRLLESCAKDAFADLFPANSISRVYSLFPDPWPKRKHLH
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCE
YRLFSSEYLRLLNNRLIFGSEALIVTDSEDYCNWMLKQLPDTGFEVENSIIPPQFDTRFE
EEHHHHHHHHHHHCCEEECCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
RKWLEQNFNTFFRILLKKARHIET
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA