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The map label for this gene is putA [C]

Identifier: 119357030

GI number: 119357030

Start: 1382417

End: 1382686

Strand: Direct

Name: putA [C]

Synonym: Cpha266_1216

Alternate gene names: 119357030

Gene position: 1382417-1382686 (Clockwise)

Preceding gene: 119357027

Following gene: 119357031

Centisome position: 44.11

GC content: 52.22

Gene sequence:

>270_bases
ATGTCACAGACAAAAGGCGTCAAGTTAGACGACACTACACAACAACGGCTTGCAGCTCTCGGTCGTATCCGCGACAGATC
GCCCCACTGGCTCATGTGCAGGGCAATCGAAACATACTTGGATCGCGAAGAAAAATATGAGCAGGAAAAGCGAGAGGATA
TGGAGCGTTGGGAACAGTATCAATTGACCGGCGTTGCAGTGCCCCATGAACAAGCTGCTGAATGGCTTGAAAATCTGGCG
CAAGGGAAGGTGACGGCGTGCCCCGGATAA

Upstream 100 bases:

>100_bases
TCAAGAGGTTACCCCATACATCCCAAATATTTCAAACGGCAAAACTCAATCTTCTACGCTATTGCACTGCGTCCCCTCTA
ATCTCAAAGGAGATTTGATC

Downstream 100 bases:

>100_bases
AATGGCTTCCGGATGCATTGTCCGATGTCGAGCGGCTTCATGCTTTCCTGCATGAAAAAAGCCCCGATGCGGCAGCACGA
GCGGCAAGGGTCATTCTTGA

Product: hypothetical protein

Products: 1-Pyrroline-5-carboxylate; FADH2; H+ [C]

Alternate protein names: None

Number of amino acids: Translated: 89; Mature: 88

Protein sequence:

>89_residues
MSQTKGVKLDDTTQQRLAALGRIRDRSPHWLMCRAIETYLDREEKYEQEKREDMERWEQYQLTGVAVPHEQAAEWLENLA
QGKVTACPG

Sequences:

>Translated_89_residues
MSQTKGVKLDDTTQQRLAALGRIRDRSPHWLMCRAIETYLDREEKYEQEKREDMERWEQYQLTGVAVPHEQAAEWLENLA
QGKVTACPG
>Mature_88_residues
SQTKGVKLDDTTQQRLAALGRIRDRSPHWLMCRAIETYLDREEKYEQEKREDMERWEQYQLTGVAVPHEQAAEWLENLAQ
GKVTACPG

Specific function: Oxidizes Proline To Glutamate For Use As A Carbon And Nitrogen Source And Also Function As A Transcriptional Repressor Of The Put Operon. [C]

COG id: COG3905

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.99.8; 1.5.1.12 [C]

Molecular weight: Translated: 10420; Mature: 10289

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTKGVKLDDTTQQRLAALGRIRDRSPHWLMCRAIETYLDREEKYEQEKREDMERWEQY
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
QLTGVAVPHEQAAEWLENLAQGKVTACPG
HHHCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQTKGVKLDDTTQQRLAALGRIRDRSPHWLMCRAIETYLDREEKYEQEKREDMERWEQY
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
QLTGVAVPHEQAAEWLENLAQGKVTACPG
HHHCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 45 {@PROL01.txt*L-proline!}} [C]

Substrates: FAD; L-Proline [C]

Specific reaction: FAD + L-Proline --> 1-Pyrroline-5-carboxylate + FADH2 + H+ [C]

General reaction: Redox reaction [C]

Inhibitor: L-Azetidine-2-carboxylate; Lactate; Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA