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The map label for this gene is hsdM [H]

Identifier: 119356950

GI number: 119356950

Start: 1280656

End: 1282317

Strand: Direct

Name: hsdM [H]

Synonym: Cpha266_1130

Alternate gene names: 119356950

Gene position: 1280656-1282317 (Clockwise)

Preceding gene: 119356949

Following gene: 119356951

Centisome position: 40.86

GC content: 51.74

Gene sequence:

>1662_bases
ATGCTCCAGCACAACCCTGAACTGAAATCAAAAATAGATCAACTCTGGAACAAATTCTGGAGCGGCGGCATATCCAATCC
ACTCACCGCCATCGAACAGATCACCTATCTGCTCTTCATGAAGCGGATGGACGATCAGGATCAGGAAAAGCAGGCAAGCG
CCGAATGGGCGGGCGAAACCTACACCTCAAAATTCAAGGGAGTGTGGATACCGCAGGAATACCGTGGCAAATCAAATTCA
TTCAATTATGCGATAGACAAAAGCACCCTTCGCTGGAGTGAATTCAAACACATGCAGGCTGAAGAGATGCTCACCCATGT
GCAGACCAAAGTGTTCCCTTATCTCAAGGATCTCAACGGTGCCGAGTCGCAATTCAGCCACCATATGAAAAATGCAGTCT
TCATCATTCCCAAGCCATCGCTTCTCGTTGAAGCCGTGAAAACGGTTGACGAGATCTTTGAGGTGATGGAGAAAGACTCG
AATGAAAAGGGGCAGGCATTTCAGGATATTCAGGGCGACGTCTATGAATTTCTGCTCTCCGAAATTGCCTCCGCCGGTAA
AAACGGGCAGTTCCGCACCCCTCGCCACATTATCAAGATGATGGCCGATCTGGTCGAGCCAAAGCTTGGCCACACCATTG
CCGATCCAGCATGTGGCACAGGCGGATTTCTGCTCGGGGCCTATCAATACATCGTAACGCAACTGGCGATTCGCGCCGGA
AACAAGGATCTCGTGGCGGACGAAGACGGATTTCTCCGCACCTCCGTTTCGGCGGGGCTTACCGAACAGGCAAAAAACAT
TCTCGGCAGGACGCTCTTCGGCTACGATATCGACAGCACCATGGTTCGCCTTGCGCTCATGAACCTCATGATGCACGGCA
TAGACGAACCGGAAATTGACTACAAAGACACTTTGAGCAAGAGCTTTACCGAAGAGTCCTGCTACGACATCATCATGGCT
AACCCTCCCTTTACCGGCAGCATCGACAAAAGCGACATCAACGAAAGCTTTACCCTTTCCACCACCAAAACAGAGCTGCT
CTTTGTCGAAAACATCTACCGGCTGCTGAAAAAGGGTGGAACCGCCTGCGTTATTGTACCCCAGGGCGTCCTCTTTGGCT
CCGGCGGCGCCTTCAAGGCACTGCGCAAGCTGCTGGTTGAGCGTTGCGACCTGAAAGCAGTTATCACCATGCCGAGCGGA
GTCTTCAAGCCTTATGCCGGAGTCTCAACCTCCATCCTGCTCTTTACCAAAGTATGGGGCCCGCTCGACAAGGTCACCAA
ACCGGCGACAGAGCAGGTCTGGTTCTACGACATGCAGTCGGACGGCTACTCGCTTGACGACAAGCGCAGCAAGCAGGATG
GTTTCGGCGATCTGCTTGATATTGTGGAGAAATACAAACAGCGAAGCGTTGAGCACGACACCGACAGAACCCGAAAGTTC
TTTTTTGTGCCGCGCAGTGAAATTGAGAGCGAAGGAGGATATGATCTCTCCCTTTCCCGTTACAAAACAGATGTTTTTGA
AGAGGTCGTCTACGAAAGCCCGTCGGTGATTCTCGGCAAACTGATTGCAGCAGAGGTGGGGGAGATTGACGAGACTGAAC
TTGCCAACATTCAGAGCGGCATTGTACGGGAGTTGCTTGAGCTCAAGGGGATGATTGGATGA

Upstream 100 bases:

>100_bases
CAACGCTACAAAACAACCGCGCAAGGTCAAACATTTTTGAAGCAGAACAGACAATGAGAACGCAAAACACATTTGCCAAC
AATAGTACAGATACAAGACG

Downstream 100 bases:

>100_bases
AAACAGTTAAACTCGGCACTTTCATCACGATATCCAAAGGGAAGAAGCATACTCTTTCTGAAATGCCATCATCTCAATCA
ATACGAATGCTTGGTATTGA

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MLQHNPELKSKIDQLWNKFWSGGISNPLTAIEQITYLLFMKRMDDQDQEKQASAEWAGETYTSKFKGVWIPQEYRGKSNS
FNYAIDKSTLRWSEFKHMQAEEMLTHVQTKVFPYLKDLNGAESQFSHHMKNAVFIIPKPSLLVEAVKTVDEIFEVMEKDS
NEKGQAFQDIQGDVYEFLLSEIASAGKNGQFRTPRHIIKMMADLVEPKLGHTIADPACGTGGFLLGAYQYIVTQLAIRAG
NKDLVADEDGFLRTSVSAGLTEQAKNILGRTLFGYDIDSTMVRLALMNLMMHGIDEPEIDYKDTLSKSFTEESCYDIIMA
NPPFTGSIDKSDINESFTLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGGAFKALRKLLVERCDLKAVITMPSG
VFKPYAGVSTSILLFTKVWGPLDKVTKPATEQVWFYDMQSDGYSLDDKRSKQDGFGDLLDIVEKYKQRSVEHDTDRTRKF
FFVPRSEIESEGGYDLSLSRYKTDVFEEVVYESPSVILGKLIAAEVGEIDETELANIQSGIVRELLELKGMIG

Sequences:

>Translated_553_residues
MLQHNPELKSKIDQLWNKFWSGGISNPLTAIEQITYLLFMKRMDDQDQEKQASAEWAGETYTSKFKGVWIPQEYRGKSNS
FNYAIDKSTLRWSEFKHMQAEEMLTHVQTKVFPYLKDLNGAESQFSHHMKNAVFIIPKPSLLVEAVKTVDEIFEVMEKDS
NEKGQAFQDIQGDVYEFLLSEIASAGKNGQFRTPRHIIKMMADLVEPKLGHTIADPACGTGGFLLGAYQYIVTQLAIRAG
NKDLVADEDGFLRTSVSAGLTEQAKNILGRTLFGYDIDSTMVRLALMNLMMHGIDEPEIDYKDTLSKSFTEESCYDIIMA
NPPFTGSIDKSDINESFTLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGGAFKALRKLLVERCDLKAVITMPSG
VFKPYAGVSTSILLFTKVWGPLDKVTKPATEQVWFYDMQSDGYSLDDKRSKQDGFGDLLDIVEKYKQRSVEHDTDRTRKF
FFVPRSEIESEGGYDLSLSRYKTDVFEEVVYESPSVILGKLIAAEVGEIDETELANIQSGIVRELLELKGMIG
>Mature_553_residues
MLQHNPELKSKIDQLWNKFWSGGISNPLTAIEQITYLLFMKRMDDQDQEKQASAEWAGETYTSKFKGVWIPQEYRGKSNS
FNYAIDKSTLRWSEFKHMQAEEMLTHVQTKVFPYLKDLNGAESQFSHHMKNAVFIIPKPSLLVEAVKTVDEIFEVMEKDS
NEKGQAFQDIQGDVYEFLLSEIASAGKNGQFRTPRHIIKMMADLVEPKLGHTIADPACGTGGFLLGAYQYIVTQLAIRAG
NKDLVADEDGFLRTSVSAGLTEQAKNILGRTLFGYDIDSTMVRLALMNLMMHGIDEPEIDYKDTLSKSFTEESCYDIIMA
NPPFTGSIDKSDINESFTLSTTKTELLFVENIYRLLKKGGTACVIVPQGVLFGSGGAFKALRKLLVERCDLKAVITMPSG
VFKPYAGVSTSILLFTKVWGPLDKVTKPATEQVWFYDMQSDGYSLDDKRSKQDGFGDLLDIVEKYKQRSVEHDTDRTRKF
FFVPRSEIESEGGYDLSLSRYKTDVFEEVVYESPSVILGKLIAAEVGEIDETELANIQSGIVRELLELKGMIG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=447, Percent_Identity=27.7404921700224, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 62237; Mature: 62237

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQHNPELKSKIDQLWNKFWSGGISNPLTAIEQITYLLFMKRMDDQDQEKQASAEWAGET
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
YTSKFKGVWIPQEYRGKSNSFNYAIDKSTLRWSEFKHMQAEEMLTHVQTKVFPYLKDLNG
HHHCCCCEECCHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AESQFSHHMKNAVFIIPKPSLLVEAVKTVDEIFEVMEKDSNEKGQAFQDIQGDVYEFLLS
HHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EIASAGKNGQFRTPRHIIKMMADLVEPKLGHTIADPACGTGGFLLGAYQYIVTQLAIRAG
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
NKDLVADEDGFLRTSVSAGLTEQAKNILGRTLFGYDIDSTMVRLALMNLMMHGIDEPEID
CCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
YKDTLSKSFTEESCYDIIMANPPFTGSIDKSDINESFTLSTTKTELLFVENIYRLLKKGG
HHHHHHHHHCHHHHCEEEECCCCCCCCCCHHCCCCCEEEECCHHHHHHHHHHHHHHHCCC
TACVIVPQGVLFGSGGAFKALRKLLVERCDLKAVITMPSGVFKPYAGVSTSILLFTKVWG
CEEEEEECCEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHC
PLDKVTKPATEQVWFYDMQSDGYSLDDKRSKQDGFGDLLDIVEKYKQRSVEHDTDRTRKF
CHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
FFVPRSEIESEGGYDLSLSRYKTDVFEEVVYESPSVILGKLIAAEVGEIDETELANIQSG
EEEEHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
IVRELLELKGMIG
HHHHHHHHHCCCC
>Mature Secondary Structure
MLQHNPELKSKIDQLWNKFWSGGISNPLTAIEQITYLLFMKRMDDQDQEKQASAEWAGET
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
YTSKFKGVWIPQEYRGKSNSFNYAIDKSTLRWSEFKHMQAEEMLTHVQTKVFPYLKDLNG
HHHCCCCEECCHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AESQFSHHMKNAVFIIPKPSLLVEAVKTVDEIFEVMEKDSNEKGQAFQDIQGDVYEFLLS
HHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EIASAGKNGQFRTPRHIIKMMADLVEPKLGHTIADPACGTGGFLLGAYQYIVTQLAIRAG
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
NKDLVADEDGFLRTSVSAGLTEQAKNILGRTLFGYDIDSTMVRLALMNLMMHGIDEPEID
CCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
YKDTLSKSFTEESCYDIIMANPPFTGSIDKSDINESFTLSTTKTELLFVENIYRLLKKGG
HHHHHHHHHCHHHHCEEEECCCCCCCCCCHHCCCCCEEEECCHHHHHHHHHHHHHHHCCC
TACVIVPQGVLFGSGGAFKALRKLLVERCDLKAVITMPSGVFKPYAGVSTSILLFTKVWG
CEEEEEECCEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHC
PLDKVTKPATEQVWFYDMQSDGYSLDDKRSKQDGFGDLLDIVEKYKQRSVEHDTDRTRKF
CHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
FFVPRSEIESEGGYDLSLSRYKTDVFEEVVYESPSVILGKLIAAEVGEIDETELANIQSG
EEEEHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
IVRELLELKGMIG
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]