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The map label for this gene is cca [H]

Identifier: 118581706

GI number: 118581706

Start: 3642924

End: 3645569

Strand: Direct

Name: cca [H]

Synonym: Ppro_3306

Alternate gene names: 118581706

Gene position: 3642924-3645569 (Clockwise)

Preceding gene: 118581705

Following gene: 118581714

Centisome position: 90.89

GC content: 64.47

Gene sequence:

>2646_bases
ATGGACGTCATCACCACCCACGTAAACGCCGATTTCGACTGCCTTGGCTCCATGGTTGCCGCCAGCCGACTTTACCCCGG
CGCACTGCTCGCATTTCCCGGTTCCCAGGAAAAGGGGCTGCGCGACTTCATCACCCACCACCCCGACTACCTCCCCCCCC
TGACCCGCGCCAGGGAGATCGACCTGGACACCGTCACCCGGCTGATCATCGTCGACTGTCAGCAGGCCTCCCGCATCGGG
CGCTTCGCCGAACTCATCGGCCGCCCCGGCCTGGAGGTACATGTCTATGACCACCATCCTGTTCTGGCAACCAGCATCCG
CCCCCATGGCGGCGTCATCCGCCCCTGCGGCGCCGTCTCCTCGATCATAACCGGCCTGCTGCGGGAGCGGGGCATGACCC
TTACCCCCGAGGAAGCCACCCTGGTTATGCTGGGCATCCACGAGGACACCGGCCGGTTGCTGTTTCTCCCCACCACGGCG
GACGATTACCGCGCTGCCGCCTGGCTGCTGGAGCAGGGGGCACGGCTGAACTTGGTCTCCGACTTCATCAGCCTGGAGCT
GACCACCCCACAGGTAGGGCTTTTGAACACCCTGCTGAAGAATCTGAAAACAACGGATTTCAACGGCGTGAGCATCTCCA
TCGCCCACGCCTCCAGCGACAGCTACATCCCTGACATCGCCGGCCTGGCACACCTGATGCGGGACATGGAGAACCTGGAC
GCCCTGTTTCTGGTTGTTGCCATGGAGAACCGCACCTATCTGGTGGCGCGAAGCCGCATACCCGAAGTCAATGTGGGTGA
GATCATGCGCCGCTTCCAGGGAGGTGGCCATGCCACGGCTGCCTCGGCCACGGTCCACGATCTTTCACTCAAGCAGGTGC
TGAACAGGCTGGATGAACTGCTGCGCCTGATGGTGACCCCGCGGATCACCGCCGGCGAGATCATGTCATCCCCGGTCAAG
ACCATACCGGCAGGCATAACCATCGCCGAGGCGCGCGACCTCCTGACGCGCTACAACTGCAATGCCATGCCGGTCATGGA
CGGAGGACGCATGGCCGGCATCCTTTCCCGCAAGATCGTGGAAAAGGCGCTGTACCACGACCTGGGCCAATCGCCGGCCA
CGGAATTCATGCACACCGAATTCATGCGCGCCACACCTGCCACGCTGCTGTCCGACATCCAGGAGTACCTGGTGGAGGGG
AACCGGCGCTTCGTGCCGGTCTTCGACGGCGATGAACTGGCGGGAGCGGTCACCCGCACCGACCTGCTGCGCCACATGTA
CGGCGGCCGGCGAGAAGAGCCGGAGGCGCTCTATGACCTGGAATCCCTGGAGGCCAGCCCCAAATGCCGCTCAATCGCCG
CCCTGGTCAACCGCAGGCTGCCGGCAGCCACCATCAGGTTGCTGCGCGACCTGGGCAGGACCGGAGACGAACTAGGTCTG
CCGGTGTATGCCGTGGGAGGCTTCGTGCGCGATCTGCTGCTGGGGGTGGAAAACCTGGACCTGGACCTGACCGTGGAGGG
AGACGGCATCTTCTTCGCCGAGCGGTTCGCACGAGCAAAGGGGGGCAGGGTCCGCAGCCACCCCGCCTTCGGCACGGCGG
TGGTGGTGCTGTCGGACGGGAGCAAGCTGGATGTGGCCAGCACCAGGCTTGAATACTACGAATCCCCCGGCGTGCTCCCC
ACGGTGGAGCGTTCATCCCTGCGCCACGACCTGTACCGTCGCGATTTCACCATCAACACCCTGGCGATCTGCATCAACAG
CGACCGTTTCGGCATGGTAACCGACTTCTTCGGCGGCCAGCAGGATATCCAGCAACGCACCGTGCGGGTCCTGCACAACC
TCTCCTTCGTGGAGGACCCCACCCGCGTCTTCCGCGCCATCCGCTTCGAGCAGCGCCTCGCCTTTCGCATCGCCCCGCAC
ACCGAAAACCTGATCCGCAGCGCACTGCGCATGAAGGTGCTGGACAGGGTGGGGGGCATACGCCTGTTCAACGAACTGGT
ACTGATCCTGAACGAGCAGGAGCCCCTGGGCGCCGTACGGCGCATGGCCATGCTCGGCCTGCTCCCCTTCATCCACCCCC
AGCTGAGGCTGTCACCGGAGACGGAGCGGGCCATGACAGAATCATCCCGACTCCTGACCTGGTTTCGCCTCTTGTACCTG
AACGACCCCTGCCAGCAGTGGCAGGTGTATTTCCTGGCGTTGTGCGAGCGCCTCAGAGACGACGAGTTCCAGGAGGCCTG
CCGCCGCCTCTCCATGCCGGGCAGGTTCGTGAGTCAGGTGTTCGCCCATCGCCACCGGGCGCTGGTCATGCTGGAGACCC
TGCAGCGGCGCCTCAAACGAGCGCAGGATCTGCGCAACAGCGAGATCTACGACAGTTTCCACGGCCTGCCGCTGGAGATC
CTGCTCTACCTGGCAGCCAGGACCAAGAACGATCAGGTGCGGCGGCTGGTCTCGCTGTACATCACCCGCCTGCGCCACAT
GCGCCGCGAGCTTGACGGCAATGCACTCAAGGAACTGGGGCTCGCTCCGGGACCGAACTACAGCCGGATCATGAAGCGCC
TGCTGGATGCGCGCCTGGACGCGGAGGTCGGCACGGCAGAAGAGGAACGCACCCTGGCGCGCGAACTGATCGCCGACGCA
GGTTAG

Upstream 100 bases:

>100_bases
AATCCGTTGACAGCGTGGATGCGAGGCGCTAAAGCTTGATTCCGTTCACGCGCAACCAGTTGTGCGGCGCGCTATCAGAA
CCCGGGAGCCAGATGCAACC

Downstream 100 bases:

>100_bases
TACGGCTTCTCGTTGCCGATGGCGGTGATGTACACCGTCAGGTCGCCGTTGGGCCTGCTGATGGTGACCTCGTCGTCGAC
CCGCTTGCCCAAGAGAGCCT

Product: polynucleotide adenylyltransferase protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 881; Mature: 881

Protein sequence:

>881_residues
MDVITTHVNADFDCLGSMVAASRLYPGALLAFPGSQEKGLRDFITHHPDYLPPLTRAREIDLDTVTRLIIVDCQQASRIG
RFAELIGRPGLEVHVYDHHPVLATSIRPHGGVIRPCGAVSSIITGLLRERGMTLTPEEATLVMLGIHEDTGRLLFLPTTA
DDYRAAAWLLEQGARLNLVSDFISLELTTPQVGLLNTLLKNLKTTDFNGVSISIAHASSDSYIPDIAGLAHLMRDMENLD
ALFLVVAMENRTYLVARSRIPEVNVGEIMRRFQGGGHATAASATVHDLSLKQVLNRLDELLRLMVTPRITAGEIMSSPVK
TIPAGITIAEARDLLTRYNCNAMPVMDGGRMAGILSRKIVEKALYHDLGQSPATEFMHTEFMRATPATLLSDIQEYLVEG
NRRFVPVFDGDELAGAVTRTDLLRHMYGGRREEPEALYDLESLEASPKCRSIAALVNRRLPAATIRLLRDLGRTGDELGL
PVYAVGGFVRDLLLGVENLDLDLTVEGDGIFFAERFARAKGGRVRSHPAFGTAVVVLSDGSKLDVASTRLEYYESPGVLP
TVERSSLRHDLYRRDFTINTLAICINSDRFGMVTDFFGGQQDIQQRTVRVLHNLSFVEDPTRVFRAIRFEQRLAFRIAPH
TENLIRSALRMKVLDRVGGIRLFNELVLILNEQEPLGAVRRMAMLGLLPFIHPQLRLSPETERAMTESSRLLTWFRLLYL
NDPCQQWQVYFLALCERLRDDEFQEACRRLSMPGRFVSQVFAHRHRALVMLETLQRRLKRAQDLRNSEIYDSFHGLPLEI
LLYLAARTKNDQVRRLVSLYITRLRHMRRELDGNALKELGLAPGPNYSRIMKRLLDARLDAEVGTAEEERTLARELIADA
G

Sequences:

>Translated_881_residues
MDVITTHVNADFDCLGSMVAASRLYPGALLAFPGSQEKGLRDFITHHPDYLPPLTRAREIDLDTVTRLIIVDCQQASRIG
RFAELIGRPGLEVHVYDHHPVLATSIRPHGGVIRPCGAVSSIITGLLRERGMTLTPEEATLVMLGIHEDTGRLLFLPTTA
DDYRAAAWLLEQGARLNLVSDFISLELTTPQVGLLNTLLKNLKTTDFNGVSISIAHASSDSYIPDIAGLAHLMRDMENLD
ALFLVVAMENRTYLVARSRIPEVNVGEIMRRFQGGGHATAASATVHDLSLKQVLNRLDELLRLMVTPRITAGEIMSSPVK
TIPAGITIAEARDLLTRYNCNAMPVMDGGRMAGILSRKIVEKALYHDLGQSPATEFMHTEFMRATPATLLSDIQEYLVEG
NRRFVPVFDGDELAGAVTRTDLLRHMYGGRREEPEALYDLESLEASPKCRSIAALVNRRLPAATIRLLRDLGRTGDELGL
PVYAVGGFVRDLLLGVENLDLDLTVEGDGIFFAERFARAKGGRVRSHPAFGTAVVVLSDGSKLDVASTRLEYYESPGVLP
TVERSSLRHDLYRRDFTINTLAICINSDRFGMVTDFFGGQQDIQQRTVRVLHNLSFVEDPTRVFRAIRFEQRLAFRIAPH
TENLIRSALRMKVLDRVGGIRLFNELVLILNEQEPLGAVRRMAMLGLLPFIHPQLRLSPETERAMTESSRLLTWFRLLYL
NDPCQQWQVYFLALCERLRDDEFQEACRRLSMPGRFVSQVFAHRHRALVMLETLQRRLKRAQDLRNSEIYDSFHGLPLEI
LLYLAARTKNDQVRRLVSLYITRLRHMRRELDGNALKELGLAPGPNYSRIMKRLLDARLDAEVGTAEEERTLARELIADA
G
>Mature_881_residues
MDVITTHVNADFDCLGSMVAASRLYPGALLAFPGSQEKGLRDFITHHPDYLPPLTRAREIDLDTVTRLIIVDCQQASRIG
RFAELIGRPGLEVHVYDHHPVLATSIRPHGGVIRPCGAVSSIITGLLRERGMTLTPEEATLVMLGIHEDTGRLLFLPTTA
DDYRAAAWLLEQGARLNLVSDFISLELTTPQVGLLNTLLKNLKTTDFNGVSISIAHASSDSYIPDIAGLAHLMRDMENLD
ALFLVVAMENRTYLVARSRIPEVNVGEIMRRFQGGGHATAASATVHDLSLKQVLNRLDELLRLMVTPRITAGEIMSSPVK
TIPAGITIAEARDLLTRYNCNAMPVMDGGRMAGILSRKIVEKALYHDLGQSPATEFMHTEFMRATPATLLSDIQEYLVEG
NRRFVPVFDGDELAGAVTRTDLLRHMYGGRREEPEALYDLESLEASPKCRSIAALVNRRLPAATIRLLRDLGRTGDELGL
PVYAVGGFVRDLLLGVENLDLDLTVEGDGIFFAERFARAKGGRVRSHPAFGTAVVVLSDGSKLDVASTRLEYYESPGVLP
TVERSSLRHDLYRRDFTINTLAICINSDRFGMVTDFFGGQQDIQQRTVRVLHNLSFVEDPTRVFRAIRFEQRLAFRIAPH
TENLIRSALRMKVLDRVGGIRLFNELVLILNEQEPLGAVRRMAMLGLLPFIHPQLRLSPETERAMTESSRLLTWFRLLYL
NDPCQQWQVYFLALCERLRDDEFQEACRRLSMPGRFVSQVFAHRHRALVMLETLQRRLKRAQDLRNSEIYDSFHGLPLEI
LLYLAARTKNDQVRRLVSLYITRLRHMRRELDGNALKELGLAPGPNYSRIMKRLLDARLDAEVGTAEEERTLARELIADA
G

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=441, Percent_Identity=24.9433106575964, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789436, Length=340, Percent_Identity=27.0588235294118, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 98906; Mature: 98906

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVITTHVNADFDCLGSMVAASRLYPGALLAFPGSQEKGLRDFITHHPDYLPPLTRAREI
CCEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCHHHCCC
DLDTVTRLIIVDCQQASRIGRFAELIGRPGLEVHVYDHHPVLATSIRPHGGVIRPCGAVS
CHHHHHHHHHEECHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCEECCHHHHH
SIITGLLRERGMTLTPEEATLVMLGIHEDTGRLLFLPTTADDYRAAAWLLEQGARLNLVS
HHHHHHHHHCCCEECCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCEEHHH
DFISLELTTPQVGLLNTLLKNLKTTDFNGVSISIAHASSDSYIPDIAGLAHLMRDMENLD
HHHEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ALFLVVAMENRTYLVARSRIPEVNVGEIMRRFQGGGHATAASATVHDLSLKQVLNRLDEL
EEEEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHH
LRLMVTPRITAGEIMSSPVKTIPAGITIAEARDLLTRYNCNAMPVMDGGRMAGILSRKIV
HHHHHCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
EKALYHDLGQSPATEFMHTEFMRATPATLLSDIQEYLVEGNRRFVPVFDGDELAGAVTRT
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
DLLRHMYGGRREEPEALYDLESLEASPKCRSIAALVNRRLPAATIRLLRDLGRTGDELGL
HHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHCC
PVYAVGGFVRDLLLGVENLDLDLTVEGDGIFFAERFARAKGGRVRSHPAFGTAVVVLSDG
CHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
SKLDVASTRLEYYESPGVLPTVERSSLRHDLYRRDFTINTLAICINSDRFGMVTDFFGGQ
CEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHCCCH
QDIQQRTVRVLHNLSFVEDPTRVFRAIRFEQRLAFRIAPHTENLIRSALRMKVLDRVGGI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCHH
RLFNELVLILNEQEPLGAVRRMAMLGLLPFIHPQLRLSPETERAMTESSRLLTWFRLLYL
HHHHHHHEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NDPCQQWQVYFLALCERLRDDEFQEACRRLSMPGRFVSQVFAHRHRALVMLETLQRRLKR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQDLRNSEIYDSFHGLPLEILLYLAARTKNDQVRRLVSLYITRLRHMRRELDGNALKELG
HHHCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
LAPGPNYSRIMKRLLDARLDAEVGTAEEERTLARELIADAG
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVITTHVNADFDCLGSMVAASRLYPGALLAFPGSQEKGLRDFITHHPDYLPPLTRAREI
CCEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCHHHCCC
DLDTVTRLIIVDCQQASRIGRFAELIGRPGLEVHVYDHHPVLATSIRPHGGVIRPCGAVS
CHHHHHHHHHEECHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCEECCHHHHH
SIITGLLRERGMTLTPEEATLVMLGIHEDTGRLLFLPTTADDYRAAAWLLEQGARLNLVS
HHHHHHHHHCCCEECCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCEEHHH
DFISLELTTPQVGLLNTLLKNLKTTDFNGVSISIAHASSDSYIPDIAGLAHLMRDMENLD
HHHEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
ALFLVVAMENRTYLVARSRIPEVNVGEIMRRFQGGGHATAASATVHDLSLKQVLNRLDEL
EEEEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHH
LRLMVTPRITAGEIMSSPVKTIPAGITIAEARDLLTRYNCNAMPVMDGGRMAGILSRKIV
HHHHHCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
EKALYHDLGQSPATEFMHTEFMRATPATLLSDIQEYLVEGNRRFVPVFDGDELAGAVTRT
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
DLLRHMYGGRREEPEALYDLESLEASPKCRSIAALVNRRLPAATIRLLRDLGRTGDELGL
HHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHCC
PVYAVGGFVRDLLLGVENLDLDLTVEGDGIFFAERFARAKGGRVRSHPAFGTAVVVLSDG
CHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
SKLDVASTRLEYYESPGVLPTVERSSLRHDLYRRDFTINTLAICINSDRFGMVTDFFGGQ
CEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHCCCH
QDIQQRTVRVLHNLSFVEDPTRVFRAIRFEQRLAFRIAPHTENLIRSALRMKVLDRVGGI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCHH
RLFNELVLILNEQEPLGAVRRMAMLGLLPFIHPQLRLSPETERAMTESSRLLTWFRLLYL
HHHHHHHEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NDPCQQWQVYFLALCERLRDDEFQEACRRLSMPGRFVSQVFAHRHRALVMLETLQRRLKR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQDLRNSEIYDSFHGLPLEILLYLAARTKNDQVRRLVSLYITRLRHMRRELDGNALKELG
HHHCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
LAPGPNYSRIMKRLLDARLDAEVGTAEEERTLARELIADAG
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA