Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 118581650

GI number: 118581650

Start: 3569771

End: 3570973

Strand: Direct

Name: amtB [C]

Synonym: Ppro_3249

Alternate gene names: 118581650

Gene position: 3569771-3570973 (Clockwise)

Preceding gene: 118581648

Following gene: 118581651

Centisome position: 89.07

GC content: 63.92

Gene sequence:

>1203_bases
ATGACGGGCCTGAAAAGCAGCGCCGATGTCCTGTTTCTGATGCTGGGGGCGGCCATGGTGTTTGCCATGCACGCCGGCTT
CGCCTTTCTGGAGGTGGGCACGGTGCGCAAGAAGAACCAGGTCAACGCCTTTGTCAAGATCCTCACCGACTGGGCGGTTT
CGACAGTCGTCTACTTCCTGATCGGCTTCCCCATCTCCTACGGCATATCCTTCCTGAAACCGGCCAGCGATCTCATGGGG
CCGAACCAGGGCTACGACCTGGTGCACTACTTCTTCCTGCTCTGCTTCGCCGCCTGCATTCCCGCCATCATCTCCGGCGG
CATCGCCGAGCGGGCCAAGTTCTGGCCCCAGGTCATTGCCGGCGCCATCTTCGCCGGCCTCTGCTACCCGCTGTTCGAGT
CCTTGATCTGGGGCCAGAACGCCTCGATGCTGCAAGGGTTCTTCAAGTCCCTGGGGGGAGCGGAATTTCACGATTACGCC
GGATCGGTGGTGGTGCACTCCATAGGCGGCTGGATCGCCCTGCCGGCGGTGTTGATCCTGGCCCCCCGCATGGGGCGCTA
CGTGCGCGGCAAATCCCACCCCATCCCGGTCAGCAATATCCCCTTCCTGGCCCTGGGCTCCTGGATCCTGGCGGTGGGGT
GGTTCGGCTTCAACGTCATGAGCGCCGGCAACCTGGAAAAGATCTCTGGCCTGGTGGCGGTCAATTCCCTCTTGGCCATG
GTGGGGGGCATACTGGCCGCCCTGGTGGCAGGCAGGAACGACCCCGGCTTTGTGCACAATGGCGCCCTGGCCGGCCTGAT
CGCCGTCTGCGCCGGCAGCGACATCATGCACCCCCTGGCATCCCTGGTGGTGGGGATGATCGCCTCGCTGATCTTCGTCT
ACGGTTTCCACCTGGAGCAGGAGGTGCTGAAGATCGATGACGTTCTGGGGGTCTGGCCGCTGCACGGCGTGATCGGCTCC
TGGGGCGGCATTACCGCCGGCATCTTCGGCCAGAAGGCCCTGGGTGGAATGGGGGGGGTCAGCTTCGTCTCCCAACTGGC
AGGCTCCCTGTCGGCCATCCTCTTCGCCCTGGCCAGCGGCTTCATCGTCTACGGCATCCTCAACAAGACGGTGGGCATCA
GGTTGAGCGAAGACGAGGAATTCGCCGGCGCCGACCTCTCCATCCACTCCATCGGCGCCTATCCGGAGGAGCACGTGCGC
TGA

Upstream 100 bases:

>100_bases
GAGTTGGCCCTCATTTTGCTTAATCACCCCCCGGCATGCCCAAAGAAGGGCATATATTCACGAGATACCAACGGGGGTAT
GTATGGAGACCATGTCGACG

Downstream 100 bases:

>100_bases
GTATCCGGCATCGGTAGAACCTTTTGCAGAGGGGGTGACGCCATGTGATGCGTCACCCCCTCTTTATTCCGTCCCATCCA
GAGCCTGTGCAAGTCTCTCC

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTGLKSSADVLFLMLGAAMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWAVSTVVYFLIGFPISYGISFLKPASDLMG
PNQGYDLVHYFFLLCFAACIPAIISGGIAERAKFWPQVIAGAIFAGLCYPLFESLIWGQNASMLQGFFKSLGGAEFHDYA
GSVVVHSIGGWIALPAVLILAPRMGRYVRGKSHPIPVSNIPFLALGSWILAVGWFGFNVMSAGNLEKISGLVAVNSLLAM
VGGILAALVAGRNDPGFVHNGALAGLIAVCAGSDIMHPLASLVVGMIASLIFVYGFHLEQEVLKIDDVLGVWPLHGVIGS
WGGITAGIFGQKALGGMGGVSFVSQLAGSLSAILFALASGFIVYGILNKTVGIRLSEDEEFAGADLSIHSIGAYPEEHVR

Sequences:

>Translated_400_residues
MTGLKSSADVLFLMLGAAMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWAVSTVVYFLIGFPISYGISFLKPASDLMG
PNQGYDLVHYFFLLCFAACIPAIISGGIAERAKFWPQVIAGAIFAGLCYPLFESLIWGQNASMLQGFFKSLGGAEFHDYA
GSVVVHSIGGWIALPAVLILAPRMGRYVRGKSHPIPVSNIPFLALGSWILAVGWFGFNVMSAGNLEKISGLVAVNSLLAM
VGGILAALVAGRNDPGFVHNGALAGLIAVCAGSDIMHPLASLVVGMIASLIFVYGFHLEQEVLKIDDVLGVWPLHGVIGS
WGGITAGIFGQKALGGMGGVSFVSQLAGSLSAILFALASGFIVYGILNKTVGIRLSEDEEFAGADLSIHSIGAYPEEHVR
>Mature_399_residues
TGLKSSADVLFLMLGAAMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWAVSTVVYFLIGFPISYGISFLKPASDLMGP
NQGYDLVHYFFLLCFAACIPAIISGGIAERAKFWPQVIAGAIFAGLCYPLFESLIWGQNASMLQGFFKSLGGAEFHDYAG
SVVVHSIGGWIALPAVLILAPRMGRYVRGKSHPIPVSNIPFLALGSWILAVGWFGFNVMSAGNLEKISGLVAVNSLLAMV
GGILAALVAGRNDPGFVHNGALAGLIAVCAGSDIMHPLASLVVGMIASLIFVYGFHLEQEVLKIDDVLGVWPLHGVIGSW
GGITAGIFGQKALGGMGGVSFVSQLAGSLSAILFALASGFIVYGILNKTVGIRLSEDEEFAGADLSIHSIGAYPEEHVR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=414, Percent_Identity=30.1932367149758, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=30.715935334873, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=29.2343387470998, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=27.790973871734, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=26.6666666666667, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=437, Percent_Identity=24.7139588100687, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=23.5981308411215, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=25.4079254079254, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=30.3102625298329, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42159; Mature: 42028

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCEECCCCCCCCCCCEEECCCCCCHHHCC
>Mature Secondary Structure 
TGLKSSADVLFLMLGAAMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWAVSTVVYFL
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFPISYGISFLKPASDLMGPNQGYDLVHYFFLLCFAACIPAIISGGIAERAKFWPQVIA
HHHHHHHCHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GAIFAGLCYPLFESLIWGQNASMLQGFFKSLGGAEFHDYAGSVVVHSIGGWIALPAVLIL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHCHHHHHHHHHHH
APRMGRYVRGKSHPIPVSNIPFLALGSWILAVGWFGFNVMSAGNLEKISGLVAVNSLLAM
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VGGILAALVAGRNDPGFVHNGALAGLIAVCAGSDIMHPLASLVVGMIASLIFVYGFHLEQ
HHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLKIDDVLGVWPLHGVIGSWGGITAGIFGQKALGGMGGVSFVSQLAGSLSAILFALASG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FIVYGILNKTVGIRLSEDEEFAGADLSIHSIGAYPEEHVR
HHHHHHHHHHHCCEECCCCCCCCCCCEEECCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]