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The map label for this gene is priA [H]

Identifier: 118581024

GI number: 118581024

Start: 2840249

End: 2842483

Strand: Direct

Name: priA [H]

Synonym: Ppro_2612

Alternate gene names: 118581024

Gene position: 2840249-2842483 (Clockwise)

Preceding gene: 118581023

Following gene: 118581025

Centisome position: 70.86

GC content: 67.79

Gene sequence:

>2235_bases
ATGGCTATGCATCAAACTCCCCCCACCATCGTCGAAGTAGCCATTCCGCTCCCCCTGGACAAGAGCTTCCACTACCTGGT
TCCCCCGTCGCTGCTTGAGCAGGCGCAGCCGGGGAAGCGGGTGCTGGTCCCCTTCGGCAGCCGCAAACTCACCGGCTATC
TGCTGGCCTTCGCTCAGACGGAAGACTGCACCAGACTGAAAGAGATCATCGATGTCCTGGACAGTGATCCGCTCTGGACC
GATGGGGAACTGGAGTTCTTCCGCTGGGTGGCCTGCTACTACCTGTCCCCCCTGGGGGAGGTGATCAAGACGGCGCTCCC
CGCCGGCATCAACCCGAGGAGCGCAAAGGGTTCTGACCAGGCCGTGGCGGGAGGGAGCAGGATCAGGCGCGAGGCGCTGT
ACCTGCCCGCGGATGGCGCCATGCCGGACCGTGCCCTGGGCGCCAAGGCGGCCCAACTGCTGGAGCTGCTCAGTACGGAG
GGGGAGTTCACGGCAACGGAGCTGCGCAAACGCTTCGGCTCCTGCTCGCCCCAGCTGAAGCGGCTGATGGAGTTGGGGCT
TGTGCGCCGGGAGGAGCGGGAGCTGTTCCGCGACCCCTTCCGGGACGAGACCGTCCAGCCGGACAGGCCGCGCCAGCTCA
ACCAGCACCAGCAGGCGGCCCTGGACAGCCTGCTGAGCCGTCTGGACAGCCGGGAATTCTCCCCCTTTCTGCTGCACGGC
GTCACCGGCAGCGGCAAGACAGAGGTCTACCTGCAGGCCATCAGCCATACCCTGGCGCTGGGGATGAGCGCTCTGACCCT
GGTGCCGGAGATTTCCCTGACCCCCCAGCTGGTGCGCCGCTTCCGGGCCCGCTTCGGTGACGGCATAGCCATCCTGCACA
GCGGGCTGTCCGACGGCGAGCGCTACGACGAATGGCGCCGCATCCGGCGCGGCCTGGTCCGCATCGTCATCGGCGCCCGT
TCGGCCATCTTCGCCCCGCTGGAGCGGATCGGCATCATCGTGGTGGACGAGGAGCACGACTCCTCCTTCAAGCAGAGCGA
CGGCCTGCGCTACAACGCCCGCGACCTGGCCCTGGTCAGGGGGCGCATGGAGGGGGCGCTGGTGCTGCTGGGATCGGCCA
CCCCGCTGATCACCTCCCAGTACGCCGTGGAACAGGGGAGGCTGGGACTGCTCACCCTGCCGGAGCGGGTGCGGGGCATC
CCCATGCCAACGGTGGAAACCGTGGCCATGCAGGGGGTCAAGGGGACCATCTCCGCCCGCCTGCTCCAGGCACTGGAAGA
GACCCTGGCCAGCGGGGAACAGTCCATCATCTTCCTCAACCGCCGCGGCTTCGCCACCTTCCTGGTCTGCAGCGAGTGCG
GTCAGCCCCTGACCTGCCCCAACTGCTCCGTCACCCTCACCTTTCACCGCGGCCGCGGCCAGAGTGTCTGCCACTACTGC
GACTTTGCCATCCCGGCCCCCGGCATCTGCCCCGGCTGCGGCTGCCCGGAATTGAAGGAACTGGGTGCCGGCACCGAACG
GGTGGAGCACGAGCTGCGTGAACTGCTGCCGCACGCCCGCATCCTGCGCATGGACAGCGACACCACCACCGGCAGGGGGA
GCCACGACCGGCTGCTGAGACGCATGAACGACGGAAGCGCCGACATCCTGATCGGCACCCAGATGATCACCAAGGGGCAT
GACTTCCCCAGCGTGACCCTGGTGGGGGTGATCAACGCCGAGGCCAGCCTGCACATGCCCGACTTCAGGAGTTCCGAACG
CACCTTCCAGCTGCTCTGCCAGGTATTCGGCCGTGCCGGCCGCGGGGACACACCCGGCCGGGTGATCCTGCAGGCCCTGG
ACCCGGGGCATTACGCCATCCAGTGCGCCATTGACCACGACAGCGCCGGCTTCTACCGCCAGGAACTGGAGTTCAGGCGC
GAGGCGGGCTACCCCCCCTTTTCCTTCCTGGCATCCCTGGCCCTGTCAGGCACCTGCGAGGAGGCGGTGGAGCAGCGCGC
CGAGGCTGCCGCCCGTCTGCTGCTGAACATCAAACGGGAGCAGAAGCTGCGCACCGAACTCCTGGGGCCCGCGCCGGCGC
CGCTGTACCGCCTGCGCGGCCGTTTCCGGCGCCGCATCCTGATCAAATCCGCCTCCCGCTCCGAACTGCGCCGGCTGATC
GTCGCCTGGCGCGACCAGCGGGACTCCTCCAGCGTTCGGGAAGCCATGGATATCGATCCGCTGGACATGAGCTGA

Upstream 100 bases:

>100_bases
CCTTCCAATCCCTGGCCGGCGAGGTGCAACGGTTGATCAATAAGGGGAAGTAAAGGGGGAGCTCCCCCTTTACTTCCCCT
CCCCTTCTCTGCTATCACTC

Downstream 100 bases:

>100_bases
CCAGCCAAGCGCAACGATGTTCCCCTCTTTCCCCTTGCGCCTGCCGGGCAAGACAGGATACATATCAGGACAATTAGTGT
CCGAATTATCGCATTAGGCC

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MAMHQTPPTIVEVAIPLPLDKSFHYLVPPSLLEQAQPGKRVLVPFGSRKLTGYLLAFAQTEDCTRLKEIIDVLDSDPLWT
DGELEFFRWVACYYLSPLGEVIKTALPAGINPRSAKGSDQAVAGGSRIRREALYLPADGAMPDRALGAKAAQLLELLSTE
GEFTATELRKRFGSCSPQLKRLMELGLVRREERELFRDPFRDETVQPDRPRQLNQHQQAALDSLLSRLDSREFSPFLLHG
VTGSGKTEVYLQAISHTLALGMSALTLVPEISLTPQLVRRFRARFGDGIAILHSGLSDGERYDEWRRIRRGLVRIVIGAR
SAIFAPLERIGIIVVDEEHDSSFKQSDGLRYNARDLALVRGRMEGALVLLGSATPLITSQYAVEQGRLGLLTLPERVRGI
PMPTVETVAMQGVKGTISARLLQALEETLASGEQSIIFLNRRGFATFLVCSECGQPLTCPNCSVTLTFHRGRGQSVCHYC
DFAIPAPGICPGCGCPELKELGAGTERVEHELRELLPHARILRMDSDTTTGRGSHDRLLRRMNDGSADILIGTQMITKGH
DFPSVTLVGVINAEASLHMPDFRSSERTFQLLCQVFGRAGRGDTPGRVILQALDPGHYAIQCAIDHDSAGFYRQELEFRR
EAGYPPFSFLASLALSGTCEEAVEQRAEAAARLLLNIKREQKLRTELLGPAPAPLYRLRGRFRRRILIKSASRSELRRLI
VAWRDQRDSSSVREAMDIDPLDMS

Sequences:

>Translated_744_residues
MAMHQTPPTIVEVAIPLPLDKSFHYLVPPSLLEQAQPGKRVLVPFGSRKLTGYLLAFAQTEDCTRLKEIIDVLDSDPLWT
DGELEFFRWVACYYLSPLGEVIKTALPAGINPRSAKGSDQAVAGGSRIRREALYLPADGAMPDRALGAKAAQLLELLSTE
GEFTATELRKRFGSCSPQLKRLMELGLVRREERELFRDPFRDETVQPDRPRQLNQHQQAALDSLLSRLDSREFSPFLLHG
VTGSGKTEVYLQAISHTLALGMSALTLVPEISLTPQLVRRFRARFGDGIAILHSGLSDGERYDEWRRIRRGLVRIVIGAR
SAIFAPLERIGIIVVDEEHDSSFKQSDGLRYNARDLALVRGRMEGALVLLGSATPLITSQYAVEQGRLGLLTLPERVRGI
PMPTVETVAMQGVKGTISARLLQALEETLASGEQSIIFLNRRGFATFLVCSECGQPLTCPNCSVTLTFHRGRGQSVCHYC
DFAIPAPGICPGCGCPELKELGAGTERVEHELRELLPHARILRMDSDTTTGRGSHDRLLRRMNDGSADILIGTQMITKGH
DFPSVTLVGVINAEASLHMPDFRSSERTFQLLCQVFGRAGRGDTPGRVILQALDPGHYAIQCAIDHDSAGFYRQELEFRR
EAGYPPFSFLASLALSGTCEEAVEQRAEAAARLLLNIKREQKLRTELLGPAPAPLYRLRGRFRRRILIKSASRSELRRLI
VAWRDQRDSSSVREAMDIDPLDMS
>Mature_743_residues
AMHQTPPTIVEVAIPLPLDKSFHYLVPPSLLEQAQPGKRVLVPFGSRKLTGYLLAFAQTEDCTRLKEIIDVLDSDPLWTD
GELEFFRWVACYYLSPLGEVIKTALPAGINPRSAKGSDQAVAGGSRIRREALYLPADGAMPDRALGAKAAQLLELLSTEG
EFTATELRKRFGSCSPQLKRLMELGLVRREERELFRDPFRDETVQPDRPRQLNQHQQAALDSLLSRLDSREFSPFLLHGV
TGSGKTEVYLQAISHTLALGMSALTLVPEISLTPQLVRRFRARFGDGIAILHSGLSDGERYDEWRRIRRGLVRIVIGARS
AIFAPLERIGIIVVDEEHDSSFKQSDGLRYNARDLALVRGRMEGALVLLGSATPLITSQYAVEQGRLGLLTLPERVRGIP
MPTVETVAMQGVKGTISARLLQALEETLASGEQSIIFLNRRGFATFLVCSECGQPLTCPNCSVTLTFHRGRGQSVCHYCD
FAIPAPGICPGCGCPELKELGAGTERVEHELRELLPHARILRMDSDTTTGRGSHDRLLRRMNDGSADILIGTQMITKGHD
FPSVTLVGVINAEASLHMPDFRSSERTFQLLCQVFGRAGRGDTPGRVILQALDPGHYAIQCAIDHDSAGFYRQELEFRRE
AGYPPFSFLASLALSGTCEEAVEQRAEAAARLLLNIKREQKLRTELLGPAPAPLYRLRGRFRRRILIKSASRSELRRLIV
AWRDQRDSSSVREAMDIDPLDMS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=746, Percent_Identity=36.8632707774799, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82547; Mature: 82416

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMHQTPPTIVEVAIPLPLDKSFHYLVPPSLLEQAQPGKRVLVPFGSRKLTGYLLAFAQT
CCCCCCCCCEEEEEECCCCCCCCCEECCHHHHHHCCCCCEEEEECCCCHHEEEEEEEECC
EDCTRLKEIIDVLDSDPLWTDGELEFFRWVACYYLSPLGEVIKTALPAGINPRSAKGSDQ
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AVAGGSRIRREALYLPADGAMPDRALGAKAAQLLELLSTEGEFTATELRKRFGSCSPQLK
HHCCHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
RLMELGLVRREERELFRDPFRDETVQPDRPRQLNQHQQAALDSLLSRLDSREFSPFLLHG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
VTGSGKTEVYLQAISHTLALGMSALTLVPEISLTPQLVRRFRARFGDGIAILHSGLSDGE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCH
RYDEWRRIRRGLVRIVIGARSAIFAPLERIGIIVVDEEHDSSFKQSDGLRYNARDLALVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHCCCCEECCHHHHHHH
GRMEGALVLLGSATPLITSQYAVEQGRLGLLTLPERVRGIPMPTVETVAMQGVKGTISAR
HCCCCEEEEEECCCCHHHHHHHHHCCCCCEEECHHHHCCCCCCHHHHHHHCCCCHHHHHH
LLQALEETLASGEQSIIFLNRRGFATFLVCSECGQPLTCPNCSVTLTFHRGRGQSVCHYC
HHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHCCCCCCCCCCEEEEEEECCCCCCHHHHH
DFAIPAPGICPGCGCPELKELGAGTERVEHELRELLPHARILRMDSDTTTGRGSHDRLLR
CCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHH
RMNDGSADILIGTQMITKGHDFPSVTLVGVINAEASLHMPDFRSSERTFQLLCQVFGRAG
HHCCCCCEEEEEHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCCC
RGDTPGRVILQALDPGHYAIQCAIDHDSAGFYRQELEFRREAGYPPFSFLASLALSGTCE
CCCCCHHEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHH
EAVEQRAEAAARLLLNIKREQKLRTELLGPAPAPLYRLRGRFRRRILIKSASRSELRRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
VAWRDQRDSSSVREAMDIDPLDMS
HHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AMHQTPPTIVEVAIPLPLDKSFHYLVPPSLLEQAQPGKRVLVPFGSRKLTGYLLAFAQT
CCCCCCCCEEEEEECCCCCCCCCEECCHHHHHHCCCCCEEEEECCCCHHEEEEEEEECC
EDCTRLKEIIDVLDSDPLWTDGELEFFRWVACYYLSPLGEVIKTALPAGINPRSAKGSDQ
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AVAGGSRIRREALYLPADGAMPDRALGAKAAQLLELLSTEGEFTATELRKRFGSCSPQLK
HHCCHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
RLMELGLVRREERELFRDPFRDETVQPDRPRQLNQHQQAALDSLLSRLDSREFSPFLLHG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
VTGSGKTEVYLQAISHTLALGMSALTLVPEISLTPQLVRRFRARFGDGIAILHSGLSDGE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCH
RYDEWRRIRRGLVRIVIGARSAIFAPLERIGIIVVDEEHDSSFKQSDGLRYNARDLALVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHCCCCEECCHHHHHHH
GRMEGALVLLGSATPLITSQYAVEQGRLGLLTLPERVRGIPMPTVETVAMQGVKGTISAR
HCCCCEEEEEECCCCHHHHHHHHHCCCCCEEECHHHHCCCCCCHHHHHHHCCCCHHHHHH
LLQALEETLASGEQSIIFLNRRGFATFLVCSECGQPLTCPNCSVTLTFHRGRGQSVCHYC
HHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHCCCCCCCCCCEEEEEEECCCCCCHHHHH
DFAIPAPGICPGCGCPELKELGAGTERVEHELRELLPHARILRMDSDTTTGRGSHDRLLR
CCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHH
RMNDGSADILIGTQMITKGHDFPSVTLVGVINAEASLHMPDFRSSERTFQLLCQVFGRAG
HHCCCCCEEEEEHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCCC
RGDTPGRVILQALDPGHYAIQCAIDHDSAGFYRQELEFRREAGYPPFSFLASLALSGTCE
CCCCCHHEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHH
EAVEQRAEAAARLLLNIKREQKLRTELLGPAPAPLYRLRGRFRRRILIKSASRSELRRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
VAWRDQRDSSSVREAMDIDPLDMS
HHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]