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The map label for this gene is aspA [H]

Identifier: 118578989

GI number: 118578989

Start: 586439

End: 587842

Strand: Direct

Name: aspA [H]

Synonym: Ppro_0550

Alternate gene names: 118578989

Gene position: 586439-587842 (Clockwise)

Preceding gene: 118578986

Following gene: 118578990

Centisome position: 14.63

GC content: 64.53

Gene sequence:

>1404_bases
ATGTGTATGCGTCGGGAACGGGATTCGCTGGGAGAAATGGATGTTCCGGCATCGGCATACTATGGCGCCCAGACGGCACG
GGCCGTACGGAACTTTCCCCTCTCGGGGCTTAAGCCCCACCCGGCCTATGTCTGGGCCGTGGTGATTATCAAGAAGTGCG
CTGCCCGGGCCAACATGTCCACCGGCAGGCTGCCCGCTGCCATCGGTGACGCCATCGTCGCGGCGGCTGACGAGGTCCTG
GGCGGCGGACTGGCCGATCAGTTCGTGGTTGATCCCTTTCAGGCCGGGGCAGGAACCTCCCACAACATGAATGTCAACGA
GGTGCTGGCCAACCGCGCCCTGGAACTGCTCGGTCGCCAACGGGGCGATTTTTCGTCTCTGCATCCCAATGACCATGTCA
ACATGGCCCAGTCCACCAATGACGTCATCCCCACCGCTATCCGGCTGGCATCCCTGGAGCTGTTGGAGCCGCTGCTGGAA
GCGCTGACCGGGCTGGTGAACAGCCTGGCGGCAAAGGGGGAGGAGTTCGATCGGATTCTCAAATCGGGGCGAACGCATCT
GCAGGACGCCGTCCCGATCCGCCTGGGGCAGGAGTTTTGCGCCTATGCCGCCGCCGTCAGGAGAAATGGCGAAGGCCTCG
AGGCGTGCATTCCGGCCCTGCAGGAGCTGGGCATTGGCGGCAACGCAGTCGGAAGCGGCCTGAATGCCGAACCGGCCTAT
ATCAGGGCGATGCTCGAGGAACTGGCCATTGCCACCGGCTTTCAGCTGAAGGCGGCGCCCGACCTGTTCGAGGCCATGCA
GAACATGGACCCCTTCTTGGCGCTCTCATCCGCCCTGCGCCGCACGGCGGTGACGGTTGGCCGCATCGCCAACGACCTGC
GCCTGCTCTCCTCCGGGCCGCGCACCGGGCTGGACGAGATCCGCCTGCCGGCGGTCCAGCCCGGCTCGTCGATCATGCCG
GGCAAGGTCAACCCCTCCATGGCGGAGATGACCAACATGGTCTGCTACCAGGTCATGGGGTGCGACCAGGCGGTGATGCT
GGCGGCCCAGGCGGGCCAGCTGGAGCTGAACGTGATGATGCCGCTCATTGCCTATAACCTGCTGTTTTCACTGGAGCTGT
TAAAGAATTGCCTGGGCAGGTTCAGCGACTCCTGCGTCAGCGGCATTATCGCCAATGGGGAGCGCTGTCGACGCTACCTG
GAGGAATCCCTGGGGCTGGTGACGGTTCTGGCGCCATCCATCGGCTACGACGCCGCTGCCGAGGTGGCCAAGGAATCCAT
GGCCACTGGCCGCTCCATCCGCGAGATCGTCCTGTCGCGGGCCTTGATGACTCCGGCGGAGTTGGATCAGGCCATGTGTC
CCGGGCACCTGACCGAACCGGGCCTGCCCCTGGGGAGGAAATGA

Upstream 100 bases:

>100_bases
GTACCATGCTCCGGCAGTAAACATGTCGCCTGCCCCCGAGTTCCGTCCGGATCGGGCGGCCTGGGCAACCATTTCCGTAA
CAGCTGAGGAGGAAGCAGTC

Downstream 100 bases:

>100_bases
TCTGCAGCCGCTGCGGTGCCTGCCCTTTGATGCGACAGGTATGGCCTGGAAACGTGCGGGTCATGAGTGCAGCTCTGCAC
CGTTACCCTCAATGTGCAAT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MCMRRERDSLGEMDVPASAYYGAQTARAVRNFPLSGLKPHPAYVWAVVIIKKCAARANMSTGRLPAAIGDAIVAAADEVL
GGGLADQFVVDPFQAGAGTSHNMNVNEVLANRALELLGRQRGDFSSLHPNDHVNMAQSTNDVIPTAIRLASLELLEPLLE
ALTGLVNSLAAKGEEFDRILKSGRTHLQDAVPIRLGQEFCAYAAAVRRNGEGLEACIPALQELGIGGNAVGSGLNAEPAY
IRAMLEELAIATGFQLKAAPDLFEAMQNMDPFLALSSALRRTAVTVGRIANDLRLLSSGPRTGLDEIRLPAVQPGSSIMP
GKVNPSMAEMTNMVCYQVMGCDQAVMLAAQAGQLELNVMMPLIAYNLLFSLELLKNCLGRFSDSCVSGIIANGERCRRYL
EESLGLVTVLAPSIGYDAAAEVAKESMATGRSIREIVLSRALMTPAELDQAMCPGHLTEPGLPLGRK

Sequences:

>Translated_467_residues
MCMRRERDSLGEMDVPASAYYGAQTARAVRNFPLSGLKPHPAYVWAVVIIKKCAARANMSTGRLPAAIGDAIVAAADEVL
GGGLADQFVVDPFQAGAGTSHNMNVNEVLANRALELLGRQRGDFSSLHPNDHVNMAQSTNDVIPTAIRLASLELLEPLLE
ALTGLVNSLAAKGEEFDRILKSGRTHLQDAVPIRLGQEFCAYAAAVRRNGEGLEACIPALQELGIGGNAVGSGLNAEPAY
IRAMLEELAIATGFQLKAAPDLFEAMQNMDPFLALSSALRRTAVTVGRIANDLRLLSSGPRTGLDEIRLPAVQPGSSIMP
GKVNPSMAEMTNMVCYQVMGCDQAVMLAAQAGQLELNVMMPLIAYNLLFSLELLKNCLGRFSDSCVSGIIANGERCRRYL
EESLGLVTVLAPSIGYDAAAEVAKESMATGRSIREIVLSRALMTPAELDQAMCPGHLTEPGLPLGRK
>Mature_467_residues
MCMRRERDSLGEMDVPASAYYGAQTARAVRNFPLSGLKPHPAYVWAVVIIKKCAARANMSTGRLPAAIGDAIVAAADEVL
GGGLADQFVVDPFQAGAGTSHNMNVNEVLANRALELLGRQRGDFSSLHPNDHVNMAQSTNDVIPTAIRLASLELLEPLLE
ALTGLVNSLAAKGEEFDRILKSGRTHLQDAVPIRLGQEFCAYAAAVRRNGEGLEACIPALQELGIGGNAVGSGLNAEPAY
IRAMLEELAIATGFQLKAAPDLFEAMQNMDPFLALSSALRRTAVTVGRIANDLRLLSSGPRTGLDEIRLPAVQPGSSIMP
GKVNPSMAEMTNMVCYQVMGCDQAVMLAAQAGQLELNVMMPLIAYNLLFSLELLKNCLGRFSDSCVSGIIANGERCRRYL
EESLGLVTVLAPSIGYDAAAEVAKESMATGRSIREIVLSRALMTPAELDQAMCPGHLTEPGLPLGRK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=44.2764578833693, Blast_Score=378, Evalue=1e-105,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=46.2526766595289, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787896, Length=453, Percent_Identity=44.1501103752759, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790398, Length=303, Percent_Identity=27.3927392739274, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=44.5887445887446, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.5076452599388, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=44.5887445887446, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=44.4924406047516, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=44.4924406047516, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=42.0258620689655, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24662684, Length=436, Percent_Identity=44.0366972477064, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24583245, Length=438, Percent_Identity=39.2694063926941, Blast_Score=312, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 49742; Mature: 49742

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMRRERDSLGEMDVPASAYYGAQTARAVRNFPLSGLKPHPAYVWAVVIIKKCAARANMS
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TGRLPAAIGDAIVAAADEVLGGGLADQFVVDPFQAGAGTSHNMNVNEVLANRALELLGRQ
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCC
RGDFSSLHPNDHVNMAQSTNDVIPTAIRLASLELLEPLLEALTGLVNSLAAKGEEFDRIL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KSGRTHLQDAVPIRLGQEFCAYAAAVRRNGEGLEACIPALQELGIGGNAVGSGLNAEPAY
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCHHH
IRAMLEELAIATGFQLKAAPDLFEAMQNMDPFLALSSALRRTAVTVGRIANDLRLLSSGP
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTGLDEIRLPAVQPGSSIMPGKVNPSMAEMTNMVCYQVMGCDQAVMLAAQAGQLELNVMM
CCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEHHHHH
PLIAYNLLFSLELLKNCLGRFSDSCVSGIIANGERCRRYLEESLGLVTVLAPSIGYDAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHH
EVAKESMATGRSIREIVLSRALMTPAELDQAMCPGHLTEPGLPLGRK
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCMRRERDSLGEMDVPASAYYGAQTARAVRNFPLSGLKPHPAYVWAVVIIKKCAARANMS
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TGRLPAAIGDAIVAAADEVLGGGLADQFVVDPFQAGAGTSHNMNVNEVLANRALELLGRQ
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCC
RGDFSSLHPNDHVNMAQSTNDVIPTAIRLASLELLEPLLEALTGLVNSLAAKGEEFDRIL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KSGRTHLQDAVPIRLGQEFCAYAAAVRRNGEGLEACIPALQELGIGGNAVGSGLNAEPAY
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCHHH
IRAMLEELAIATGFQLKAAPDLFEAMQNMDPFLALSSALRRTAVTVGRIANDLRLLSSGP
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RTGLDEIRLPAVQPGSSIMPGKVNPSMAEMTNMVCYQVMGCDQAVMLAAQAGQLELNVMM
CCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEHHHHH
PLIAYNLLFSLELLKNCLGRFSDSCVSGIIANGERCRRYLEESLGLVTVLAPSIGYDAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHH
EVAKESMATGRSIREIVLSRALMTPAELDQAMCPGHLTEPGLPLGRK
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]