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The map label for this gene is rlmN

Identifier: 118578868

GI number: 118578868

Start: 448289

End: 449332

Strand: Direct

Name: rlmN

Synonym: Ppro_0427

Alternate gene names: 118578868

Gene position: 448289-449332 (Clockwise)

Preceding gene: 118578867

Following gene: 118578869

Centisome position: 11.18

GC content: 61.97

Gene sequence:

>1044_bases
ATGACCGAAAAAACCGACCTGAAAAACCTGACCCTGCCTGCTCTGGAGCAGTTTCTGCGGGGGCAGGGCAAGGAGCGCTT
CCGCGCCACCCAGGTCTTCAAATGGATCTACCAGCACGATGCCCGCAGCTTCCAGGAGATGACCAACATATCCAAGGATC
TGCGGGCGGAGCTGGAGGCGAAGGCGTACATCAGCAACCTGGAGCCGGAGGCCGTTGAGGTGGGCGGCGACGGTACGCGC
AAGTACCTGTTCGGCCTGGAAGACGGCAATTCGGTGGAGTCGGTGCTCATACCGGACGAGGGGCGCAACACCCTCTGCAT
CTCCTCCCAGGTGGGGTGCGCCATGGGGTGCGCGTTCTGTCTCACCGGGACGTTCAGGCTGACCCGCAACCTGACCACGG
CCGAGATCGTCAACCAGATCATGGCCGTGCGGCGCGATGTGGAGATACGCAACATCGTCATGATGGGCATGGGGGAGCCG
CTGCACAACCTGGATAACGTCATCCCGGCCATCCACATCATGATCGACGGTAATGGCCTGCAGCTCTCCAACCGCCGAGT
CACGGTCTCCACCTGCGGACTGGCGCCGGAGATGGAGCGACTTGGCCGGGAGTTGCCCAACGTCAATCTGGCCGTATCGC
TGAACGCCACCACCGACGAACTGCGCGACCGGATCATGCCGATCAACCGCCGCTATCCGCTGAAGGAGCTGCTCAGCGCC
TGCCGGGAGTTCCCGCTTCCCGGACGCCGCAAGGTCACCTTCGAATACGTCATGCTGGGGGGGCTGAACGACACGCTGGA
GGATGCCAAGCGGCTGTTGCGCCTGACTAGCGATATCCCCAACAAGGTCAACCTGATTCCCTTTAACGAGTTCCAGGGGT
GTGAATTCCGCTCTCCCACCCGGGCGGCCATCGATGCCTTTCACAAATACCTGATCGACCGCCATGTGACGGTGATTACC
CGCGACAGCCGCGGCAGTGACATCTCCGCTGCCTGCGGCCAGTTGAAGGGAAAGCTTGACGCGGCTAGACAGCCGACGGA
ATAG

Upstream 100 bases:

>100_bases
AGGGAGCGGAGAGGTAGTGCCGTCCCCGGGAAAGCTGCACGGGTAGTCTCCGCGGCATTACCGCGGGAAGATGAGCGAGA
TGAATCAGGAGCATTGCCCC

Downstream 100 bases:

>100_bases
AGTCTGCAAGCCTGTCTGGACGGCAGGGGGAGGGGCGGTATGGGTGAAGAGAGCAGAAGGAAATATGAGTTCTCCTGGAG
TCTTCTGGGGGACCTGGAAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTEKTDLKNLTLPALEQFLRGQGKERFRATQVFKWIYQHDARSFQEMTNISKDLRAELEAKAYISNLEPEAVEVGGDGTR
KYLFGLEDGNSVESVLIPDEGRNTLCISSQVGCAMGCAFCLTGTFRLTRNLTTAEIVNQIMAVRRDVEIRNIVMMGMGEP
LHNLDNVIPAIHIMIDGNGLQLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYPLKELLSA
CREFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQGCEFRSPTRAAIDAFHKYLIDRHVTVIT
RDSRGSDISAACGQLKGKLDAARQPTE

Sequences:

>Translated_347_residues
MTEKTDLKNLTLPALEQFLRGQGKERFRATQVFKWIYQHDARSFQEMTNISKDLRAELEAKAYISNLEPEAVEVGGDGTR
KYLFGLEDGNSVESVLIPDEGRNTLCISSQVGCAMGCAFCLTGTFRLTRNLTTAEIVNQIMAVRRDVEIRNIVMMGMGEP
LHNLDNVIPAIHIMIDGNGLQLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYPLKELLSA
CREFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQGCEFRSPTRAAIDAFHKYLIDRHVTVIT
RDSRGSDISAACGQLKGKLDAARQPTE
>Mature_346_residues
TEKTDLKNLTLPALEQFLRGQGKERFRATQVFKWIYQHDARSFQEMTNISKDLRAELEAKAYISNLEPEAVEVGGDGTRK
YLFGLEDGNSVESVLIPDEGRNTLCISSQVGCAMGCAFCLTGTFRLTRNLTTAEIVNQIMAVRRDVEIRNIVMMGMGEPL
HNLDNVIPAIHIMIDGNGLQLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYPLKELLSAC
REFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQGCEFRSPTRAAIDAFHKYLIDRHVTVITR
DSRGSDISAACGQLKGKLDAARQPTE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=350, Percent_Identity=43.7142857142857, Blast_Score=277, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PELPD (A1AL40)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900118.1
- STRING:   A1AL40
- GeneID:   4573006
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0427
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A1AL40
- ProtClustDB:   PRK14463
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 38945; Mature: 38814

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKTDLKNLTLPALEQFLRGQGKERFRATQVFKWIYQHDARSFQEMTNISKDLRAELEA
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAYISNLEPEAVEVGGDGTRKYLFGLEDGNSVESVLIPDEGRNTLCISSQVGCAMGCAFC
HHHHHCCCCCEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHH
LTGTFRLTRNLTTAEIVNQIMAVRRDVEIRNIVMMGMGEPLHNLDNVIPAIHIMIDGNGL
HHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHEEEECCCCHHHHHHHHCCEEEEEECCCCE
QLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYPLKELLSA
EECCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHH
CREFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQGCEFRSPT
HHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCH
RAAIDAFHKYLIDRHVTVITRDSRGSDISAACGQLKGKLDAARQPTE
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
TEKTDLKNLTLPALEQFLRGQGKERFRATQVFKWIYQHDARSFQEMTNISKDLRAELEA
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAYISNLEPEAVEVGGDGTRKYLFGLEDGNSVESVLIPDEGRNTLCISSQVGCAMGCAFC
HHHHHCCCCCEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHH
LTGTFRLTRNLTTAEIVNQIMAVRRDVEIRNIVMMGMGEPLHNLDNVIPAIHIMIDGNGL
HHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHEEEECCCCHHHHHHHHCCEEEEEECCCCE
QLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYPLKELLSA
EECCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHH
CREFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQGCEFRSPT
HHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCH
RAAIDAFHKYLIDRHVTVITRDSRGSDISAACGQLKGKLDAARQPTE
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA