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The map label for this gene is oprM [H]

Identifier: 118578854

GI number: 118578854

Start: 428133

End: 429566

Strand: Direct

Name: oprM [H]

Synonym: Ppro_0412

Alternate gene names: 118578854

Gene position: 428133-429566 (Clockwise)

Preceding gene: 118578853

Following gene: 118578855

Centisome position: 10.68

GC content: 62.48

Gene sequence:

>1434_bases
ATGCGCCGACTACTCGTTCTCACACTCGCAACCCTGCTTGCCGGTTGCATGATCGGCCCGGACTACCACAGACCGGTGGT
GGAAACTCCATCCTCCTTCATATACGAAGACAAGGAGGCCCGTGCCACGGCGGACACCGAATGGTGGAAGCAGTTCCAGG
ACCCGGTGCTCGATGCTCTGATTAGTGATGCCCTTACCAACAACAAGAACATCAAGATCGCTGCCGCCAACATCGAACAG
GCCTCAGGGTTCCTGACCCAGACCCGCTCCCCGCTCTTCCCGCAGCTGGGCTATGGCGCCGATGCCGGGCGTCAGCGGCT
GAGCGAAAACAACGCCATCCCCGTCTCTCCCGGTATCAACAACCCCCAGGATTACTTCCAGCTCTTCGGCGGCGCCAGCT
GGGAGCTGGATCTCTGGGGCCATGTCAGGCGCCTCTCCGAGTTCGCACGGGCCAACCTGCTGGCCAATCAGGAGGCACGC
CGCGGCGTGATTCTCACCCTGGTGGCAGCGGTGGCGGGCGACTACCTGCAGCTGCGCGGGCTGGACGAACAGCTGGTGAT
CTCAAAGCGCAACCTGGCGGCCTATGCCGATTCGGTGAAACTGTTTGAACTGCAGTTCCAGTACGGCCAGATATCCATGC
TGAACGTCCAGCAGGCCCGCACCCAGTATGAGACCGCGGCGGCAACCATATCGCAGATCGAATCCCAGATCAGCCAGCTG
GAGAATGCCTTGTCGATCCTGCTGGGCCGCAATCCGGGATCGATTCCGCGCGGCAAGACGATCCATCAGTTGGCCTCCCC
GGCTGTTCCGGCAGGTCTCCCCTCCCAGCTGCTTGAGCGCCGGCCGGACCTGGCGCAGGCCGAACAGAACCTGATCGCCG
CCAACGCCCAGATCGGCGCGGCAAAGGCGCTCTACTTCCCGACCATCTCGCTTACCGGTAATTTCGGCTTTGCCAGCGAG
AACCTCTCCGATCTCTTCAAGGGGCCGAGCAAGCTCTGGAGTTATGGCGGCTCGATTACCGGCCCTATCTTCACCGCCGG
CGCCATCTCTGGTCAGGTTAGGCAGAGCGAAGCACTACAGAAGGCGGCGCTCCAAAGGTACGAGGCTGCGATTCAGAGTG
CCTTTGCCGACGTGGAAAATGCCCTGGTCATCCGCCAGAAGTTGGCCGGACAGCTTCTGGCCCAAGAGCGGCTGGTCAAG
GCCAGCCAGGAGTACGAGCAACTGGCCAAGCTTCAGTATGATGGAGGCTACGCCCCCTACCTCACCGTTTTGAGCGCGCA
GCAGCAGCTCTTCCCCGCCGAACTCACCCTTACCCAGTTGCGCGCCTCGCTGCTTACATCCTACGCCAACCTGTACAAGG
CCATGGGTGGCGGCTGGGTGGATGCGGCCGACAGGCTGGCAGGCACGCCTCCCGCCGCGTCCCCTCGCCTGTAG

Upstream 100 bases:

>100_bases
AAAGCGAAGAAAGCGGCGGCCGAAGGGCGTCCGCCGGACACGCCGCAGGTGGCGAGCCTGACCGAGAGCCATGATATCCA
CCGGGAAGGGGGTAACTGAC

Downstream 100 bases:

>100_bases
GGAGGCGATCGAGGTCTACTGCAAGGTGGACAGCCCAGAGGATGCGTGCTTCTGGAGTGCCGCGGCAAGCATAGCGGTGA
CTTTCCCGCGTCAGCAGGTA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRLLVLTLATLLAGCMIGPDYHRPVVETPSSFIYEDKEARATADTEWWKQFQDPVLDALISDALTNNKNIKIAAANIEQ
ASGFLTQTRSPLFPQLGYGADAGRQRLSENNAIPVSPGINNPQDYFQLFGGASWELDLWGHVRRLSEFARANLLANQEAR
RGVILTLVAAVAGDYLQLRGLDEQLVISKRNLAAYADSVKLFELQFQYGQISMLNVQQARTQYETAAATISQIESQISQL
ENALSILLGRNPGSIPRGKTIHQLASPAVPAGLPSQLLERRPDLAQAEQNLIAANAQIGAAKALYFPTISLTGNFGFASE
NLSDLFKGPSKLWSYGGSITGPIFTAGAISGQVRQSEALQKAALQRYEAAIQSAFADVENALVIRQKLAGQLLAQERLVK
ASQEYEQLAKLQYDGGYAPYLTVLSAQQQLFPAELTLTQLRASLLTSYANLYKAMGGGWVDAADRLAGTPPAASPRL

Sequences:

>Translated_477_residues
MRRLLVLTLATLLAGCMIGPDYHRPVVETPSSFIYEDKEARATADTEWWKQFQDPVLDALISDALTNNKNIKIAAANIEQ
ASGFLTQTRSPLFPQLGYGADAGRQRLSENNAIPVSPGINNPQDYFQLFGGASWELDLWGHVRRLSEFARANLLANQEAR
RGVILTLVAAVAGDYLQLRGLDEQLVISKRNLAAYADSVKLFELQFQYGQISMLNVQQARTQYETAAATISQIESQISQL
ENALSILLGRNPGSIPRGKTIHQLASPAVPAGLPSQLLERRPDLAQAEQNLIAANAQIGAAKALYFPTISLTGNFGFASE
NLSDLFKGPSKLWSYGGSITGPIFTAGAISGQVRQSEALQKAALQRYEAAIQSAFADVENALVIRQKLAGQLLAQERLVK
ASQEYEQLAKLQYDGGYAPYLTVLSAQQQLFPAELTLTQLRASLLTSYANLYKAMGGGWVDAADRLAGTPPAASPRL
>Mature_477_residues
MRRLLVLTLATLLAGCMIGPDYHRPVVETPSSFIYEDKEARATADTEWWKQFQDPVLDALISDALTNNKNIKIAAANIEQ
ASGFLTQTRSPLFPQLGYGADAGRQRLSENNAIPVSPGINNPQDYFQLFGGASWELDLWGHVRRLSEFARANLLANQEAR
RGVILTLVAAVAGDYLQLRGLDEQLVISKRNLAAYADSVKLFELQFQYGQISMLNVQQARTQYETAAATISQIESQISQL
ENALSILLGRNPGSIPRGKTIHQLASPAVPAGLPSQLLERRPDLAQAEQNLIAANAQIGAAKALYFPTISLTGNFGFASE
NLSDLFKGPSKLWSYGGSITGPIFTAGAISGQVRQSEALQKAALQRYEAAIQSAFADVENALVIRQKLAGQLLAQERLVK
ASQEYEQLAKLQYDGGYAPYLTVLSAQQQLFPAELTLTQLRASLLTSYANLYKAMGGGWVDAADRLAGTPPAASPRL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=35.9139784946237, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI2367348, Length=484, Percent_Identity=25.4132231404959, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51853; Mature: 51853

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLVLTLATLLAGCMIGPDYHRPVVETPSSFIYEDKEARATADTEWWKQFQDPVLDAL
CCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCHHCCCCCHHHHHHHHHHHHHHH
ISDALTNNKNIKIAAANIEQASGFLTQTRSPLFPQLGYGADAGRQRLSENNAIPVSPGIN
HHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCC
NPQDYFQLFGGASWELDLWGHVRRLSEFARANLLANQEARRGVILTLVAAVAGDYLQLRG
CHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHCCHHEECC
LDEQLVISKRNLAAYADSVKLFELQFQYGQISMLNVQQARTQYETAAATISQIESQISQL
CCHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ENALSILLGRNPGSIPRGKTIHQLASPAVPAGLPSQLLERRPDLAQAEQNLIAANAQIGA
HHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCC
AKALYFPTISLTGNFGFASENLSDLFKGPSKLWSYGGSITGPIFTAGAISGQVRQSEALQ
HHEEEEEEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCEEHHHCCCHHHHHHHHH
KAALQRYEAAIQSAFADVENALVIRQKLAGQLLAQERLVKASQEYEQLAKLQYDGGYAPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LTVLSAQQQLFPAELTLTQLRASLLTSYANLYKAMGGGWVDAADRLAGTPPAASPRL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRRLLVLTLATLLAGCMIGPDYHRPVVETPSSFIYEDKEARATADTEWWKQFQDPVLDAL
CCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCHHCCCCCHHHHHHHHHHHHHHH
ISDALTNNKNIKIAAANIEQASGFLTQTRSPLFPQLGYGADAGRQRLSENNAIPVSPGIN
HHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCC
NPQDYFQLFGGASWELDLWGHVRRLSEFARANLLANQEARRGVILTLVAAVAGDYLQLRG
CHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHCCHHEECC
LDEQLVISKRNLAAYADSVKLFELQFQYGQISMLNVQQARTQYETAAATISQIESQISQL
CCHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ENALSILLGRNPGSIPRGKTIHQLASPAVPAGLPSQLLERRPDLAQAEQNLIAANAQIGA
HHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCC
AKALYFPTISLTGNFGFASENLSDLFKGPSKLWSYGGSITGPIFTAGAISGQVRQSEALQ
HHEEEEEEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCEEHHHCCCHHHHHHHHH
KAALQRYEAAIQSAFADVENALVIRQKLAGQLLAQERLVKASQEYEQLAKLQYDGGYAPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LTVLSAQQQLFPAELTLTQLRASLLTSYANLYKAMGGGWVDAADRLAGTPPAASPRL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]