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The map label for this gene is rnjA [H]

Identifier: 118578825

GI number: 118578825

Start: 394925

End: 396634

Strand: Direct

Name: rnjA [H]

Synonym: Ppro_0383

Alternate gene names: 118578825

Gene position: 394925-396634 (Clockwise)

Preceding gene: 118578824

Following gene: 118578826

Centisome position: 9.85

GC content: 61.81

Gene sequence:

>1710_bases
ATGTCCCGGGGCAGGAAAGATGACACCATGAATGATAATGATTTCAACGAAAACACCGATCCCCTGCGGATCATCCCCCT
GGGTGGGCTGGGTGAGATAGGCCTGAACATGATGGCCTACGAGTGCGGCGGCGACATCCTCATCGTCGACTGCGGGCTCA
TGTTCCCCGAGCCGGACATGCTGGGGATCGACTACGTCATCCCGGACATCGGCTGGCTGCGCGAGCGCGCCGGCCGCGTG
CGTGGCATCTGTCTGACCCATGGCCACGAGGACCACATCGGCGCGCTTCCCTTCGTGTTGCAGGAACTGAACGTACCGCT
CTTTGGCACGGCCCTGACGCTTGGGTTTGTGAACGAGAAGCTGCGCGAGTACAAACTGGACGATCTGGTGGAGCTGGTCA
CCGTCAGGCCCCGTCAGAGCGTGGATCTGGGCTGCTTCCGGGTCGAATTTCTGCGGGTTGCCCACTCGGTGGTTGATGGC
TGCGCCCTGGCCATCACCACGCCTGAGGGGGTCGTGATCCATACCGGCGACTTCAAGATCGACCAGACGCCGGTGGACGG
TCAGCTGACCGATCTGGCCTCCCTTTCCCGCTACGCTGAGGCCGGGGTGCTGGCGCTCCTGTCCGATTCCACCAACGTGG
AACGGGAAGGATTCACCCTCTCGGAGAAGTACGTGGGCGAGGCATTCGCCGAGATCTTCCCCAAATGCCCGGGACGGATC
ATCGTGGCCGCCTTCTCCAGCAGCATTCACCGGGTGCAGCAGGTGGTGGACGTGGCCGTTGCCCATGGACGCAAGATCCT
GCTCAATGGCCGCTCCATGATCGCCAACGTGCGCATCGCCCGTCAGCTGGGCTACCTGACCATCTCCGACAGCGACCTGA
TGGACCTGAAAGAGCTGGGCCACCTGTCGCCCGAGCAGGTCTGCATCATCTCCACCGGCAGCCAGGGGGAGCCGATGAGC
GCCCTGACCCGCATCGCCATGGATGACCATAAGCAGATCAAGCTGGAAAAAGGGGATACGGTGATCCTCTCCTCGCGGGT
GATCCCCGGCAACGAGCGCACCATCTCCGAGCTGATCAACCACCTTTACCGCCGCGGCGCCGAGGTCTTCCACGAAAAGG
TCTCCGAGGTGCATGTCTCCGGCCACGCCAGCCAGGAGGAGCTTAAGCTGATGCTCAACACGGTGCGTCCGCGCTTCTTC
ATCCCGATCCACGGCGAGTACCGCCATCTGGTCAAGCACATCCAGCTGGCCCGCAAGGTCGGTATTCCCGAGGAACGCTG
CATTCTGGCCACGAACGGTGATGTGGTCGCGTTCTACGCCGATACGGCCGCCATCGTGGAAAAGATCGATTCGGGGCGGG
TGTTCGTGGACGGCAAGGGGGTCGGCGATGTGGGCAATGTGGTGCTTAAGGACCGCAAACATCTCTCCGAAGATGGCATG
GTGGTGGTTATCATCGGCATCAACCAGTCCTCCGGTGCCCTGATTTACGGGCCTGATATCGTGTCGCGCGGTTTTGTCTT
CGAGGACGAGAGCCAGGATTACCTGGAGACCAGTCGTGCCGTCGTCTTGGAGGCATTGGACGAACTGAACGACGAGATGC
GCTGCGACAGCGAAGAGGTGAAGCAGGCCGTGCGCCAGGCGCTGCGCCGGTTTTTCAAGAAGAGCATCGAGCGGCGGCCG
GTGATCCTGCCGGTGATCATGGAGATGTAG

Upstream 100 bases:

>100_bases
GGATTGCGCGCTGCGACAGGAAGATCGCTGCCCTGGCGCCGCTCCAGATCGAGAGATAACAGCCCCTTTACGGAACAGCC
GGCGGGAGACCGCGCAGGCG

Downstream 100 bases:

>100_bases
GCCCGGCCGCTGGCGGGAATTATTGAATTACATGGGGTATGTGTGGCGCTTCTCTCTCTTCGTAACATAACCGTCGCCTT
TGGCGGCCCACCGCTTCTGG

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSRGRKDDTMNDNDFNENTDPLRIIPLGGLGEIGLNMMAYECGGDILIVDCGLMFPEPDMLGIDYVIPDIGWLRERAGRV
RGICLTHGHEDHIGALPFVLQELNVPLFGTALTLGFVNEKLREYKLDDLVELVTVRPRQSVDLGCFRVEFLRVAHSVVDG
CALAITTPEGVVIHTGDFKIDQTPVDGQLTDLASLSRYAEAGVLALLSDSTNVEREGFTLSEKYVGEAFAEIFPKCPGRI
IVAAFSSSIHRVQQVVDVAVAHGRKILLNGRSMIANVRIARQLGYLTISDSDLMDLKELGHLSPEQVCIISTGSQGEPMS
ALTRIAMDDHKQIKLEKGDTVILSSRVIPGNERTISELINHLYRRGAEVFHEKVSEVHVSGHASQEELKLMLNTVRPRFF
IPIHGEYRHLVKHIQLARKVGIPEERCILATNGDVVAFYADTAAIVEKIDSGRVFVDGKGVGDVGNVVLKDRKHLSEDGM
VVVIIGINQSSGALIYGPDIVSRGFVFEDESQDYLETSRAVVLEALDELNDEMRCDSEEVKQAVRQALRRFFKKSIERRP
VILPVIMEM

Sequences:

>Translated_569_residues
MSRGRKDDTMNDNDFNENTDPLRIIPLGGLGEIGLNMMAYECGGDILIVDCGLMFPEPDMLGIDYVIPDIGWLRERAGRV
RGICLTHGHEDHIGALPFVLQELNVPLFGTALTLGFVNEKLREYKLDDLVELVTVRPRQSVDLGCFRVEFLRVAHSVVDG
CALAITTPEGVVIHTGDFKIDQTPVDGQLTDLASLSRYAEAGVLALLSDSTNVEREGFTLSEKYVGEAFAEIFPKCPGRI
IVAAFSSSIHRVQQVVDVAVAHGRKILLNGRSMIANVRIARQLGYLTISDSDLMDLKELGHLSPEQVCIISTGSQGEPMS
ALTRIAMDDHKQIKLEKGDTVILSSRVIPGNERTISELINHLYRRGAEVFHEKVSEVHVSGHASQEELKLMLNTVRPRFF
IPIHGEYRHLVKHIQLARKVGIPEERCILATNGDVVAFYADTAAIVEKIDSGRVFVDGKGVGDVGNVVLKDRKHLSEDGM
VVVIIGINQSSGALIYGPDIVSRGFVFEDESQDYLETSRAVVLEALDELNDEMRCDSEEVKQAVRQALRRFFKKSIERRP
VILPVIMEM
>Mature_568_residues
SRGRKDDTMNDNDFNENTDPLRIIPLGGLGEIGLNMMAYECGGDILIVDCGLMFPEPDMLGIDYVIPDIGWLRERAGRVR
GICLTHGHEDHIGALPFVLQELNVPLFGTALTLGFVNEKLREYKLDDLVELVTVRPRQSVDLGCFRVEFLRVAHSVVDGC
ALAITTPEGVVIHTGDFKIDQTPVDGQLTDLASLSRYAEAGVLALLSDSTNVEREGFTLSEKYVGEAFAEIFPKCPGRII
VAAFSSSIHRVQQVVDVAVAHGRKILLNGRSMIANVRIARQLGYLTISDSDLMDLKELGHLSPEQVCIISTGSQGEPMSA
LTRIAMDDHKQIKLEKGDTVILSSRVIPGNERTISELINHLYRRGAEVFHEKVSEVHVSGHASQEELKLMLNTVRPRFFI
PIHGEYRHLVKHIQLARKVGIPEERCILATNGDVVAFYADTAAIVEKIDSGRVFVDGKGVGDVGNVVLKDRKHLSEDGMV
VVIIGINQSSGALIYGPDIVSRGFVFEDESQDYLETSRAVVLEALDELNDEMRCDSEEVKQAVRQALRRFFKKSIERRPV
ILPVIMEM

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 63032; Mature: 62901

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGRKDDTMNDNDFNENTDPLRIIPLGGLGEIGLNMMAYECGGDILIVDCGLMFPEPDM
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCEEEEEECCCCEEEEECCCCCCCCCC
LGIDYVIPDIGWLRERAGRVRGICLTHGHEDHIGALPFVLQELNVPLFGTALTLGFVNEK
CCCHHHCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHH
LREYKLDDLVELVTVRPRQSVDLGCFRVEFLRVAHSVVDGCALAITTPEGVVIHTGDFKI
HHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCEEE
DQTPVDGQLTDLASLSRYAEAGVLALLSDSTNVEREGFTLSEKYVGEAFAEIFPKCPGRI
CCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCE
IVAAFSSSIHRVQQVVDVAVAHGRKILLNGRSMIANVRIARQLGYLTISDSDLMDLKELG
EEEHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCEEEECCCCHHHHHHHC
HLSPEQVCIISTGSQGEPMSALTRIAMDDHKQIKLEKGDTVILSSRVIPGNERTISELIN
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHH
HLYRRGAEVFHEKVSEVHVSGHASQEELKLMLNTVRPRFFIPIHGEYRHLVKHIQLARKV
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHC
GIPEERCILATNGDVVAFYADTAAIVEKIDSGRVFVDGKGVGDVGNVVLKDRKHLSEDGM
CCCCCCEEEEECCCEEEEEECHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCE
VVVIIGINQSSGALIYGPDIVSRGFVFEDESQDYLETSRAVVLEALDELNDEMRCDSEEV
EEEEEEECCCCCEEEECCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCHHHH
KQAVRQALRRFFKKSIERRPVILPVIMEM
HHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SRGRKDDTMNDNDFNENTDPLRIIPLGGLGEIGLNMMAYECGGDILIVDCGLMFPEPDM
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCEEEEEECCCCEEEEECCCCCCCCCC
LGIDYVIPDIGWLRERAGRVRGICLTHGHEDHIGALPFVLQELNVPLFGTALTLGFVNEK
CCCHHHCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHH
LREYKLDDLVELVTVRPRQSVDLGCFRVEFLRVAHSVVDGCALAITTPEGVVIHTGDFKI
HHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCEEE
DQTPVDGQLTDLASLSRYAEAGVLALLSDSTNVEREGFTLSEKYVGEAFAEIFPKCPGRI
CCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCE
IVAAFSSSIHRVQQVVDVAVAHGRKILLNGRSMIANVRIARQLGYLTISDSDLMDLKELG
EEEHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCEEEECCCCHHHHHHHC
HLSPEQVCIISTGSQGEPMSALTRIAMDDHKQIKLEKGDTVILSSRVIPGNERTISELIN
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHH
HLYRRGAEVFHEKVSEVHVSGHASQEELKLMLNTVRPRFFIPIHGEYRHLVKHIQLARKV
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHC
GIPEERCILATNGDVVAFYADTAAIVEKIDSGRVFVDGKGVGDVGNVVLKDRKHLSEDGM
CCCCCCEEEEECCCEEEEEECHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCE
VVVIIGINQSSGALIYGPDIVSRGFVFEDESQDYLETSRAVVLEALDELNDEMRCDSEEV
EEEEEEECCCCCEEEECCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCHHHH
KQAVRQALRRFFKKSIERRPVILPVIMEM
HHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]