| Definition | Pelobacter propionicus DSM 2379 chromosome, complete genome. |
|---|---|
| Accession | NC_008609 |
| Length | 4,008,000 |
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The map label for this gene is yitJ [H]
Identifier: 118578725
GI number: 118578725
Start: 279642
End: 281489
Strand: Direct
Name: yitJ [H]
Synonym: Ppro_0283
Alternate gene names: 118578725
Gene position: 279642-281489 (Clockwise)
Preceding gene: 118578719
Following gene: 118578726
Centisome position: 6.98
GC content: 67.8
Gene sequence:
>1848_bases ATGAACATCCTTGAGCGACTGAAAGGGGAGGTACTGACCGGTGACGGCGCCCTGGGCACCATGCTCTACGCCCGGGGGGT CGGCCTGGACAGCAGCTTTGAGCAGCTCAACCTGACGCGCCCCGCCCTGGTGCGGGAGCTGGCCGACGACTACGTGGCCG CCGGCGCCCAGGTGATCGAGACCAACACCTTTGGCGCCAATTATACCAGACTGGCCGCCCTGGGTCTGGAGCAGCGGGTG TGGGAGATCAACCTTGCCGGGGCCCGCATCGCCCGTCAGGCCGCCGATCAGGGAAGGGATGTGCTGGTGGCCGGATCCGT CGGCCCGCTGCCGCGCATCAGGGGGGACGAGGCCGAGTCTGATCAGGAGCGCATGGCGGAGCTGTTCCGGGTCCAGTGCC AGGCCCTGGCCGAGGGGGGCGTGGACCTGCTGCTGCTGGAAACCTTTTCCTCCCTCACCCAACTGGCGGCGGCGGTCCAA GCGGCCCGCTGCACCGGTCTGACCGTGGCGGCTTCCATGGCCTTTCTGGAGGGGGGGCGCAGCGCCGACGGCGCTACGGT TGAGGAGTTCTGCGCCGCCATGGAGCGCGCCGGGGCCGACCTGGTGGGCGCCAACTGCGGCGCAGGTCCCCTGGAGCTGG TCAAGGTGGTGCGGCGGCTGGCGGCGGTCACCCATAAGCCGATCACCGCCTATGCCAACAGCGGTTTCCCCGAATACCAC GACGGTCGCTTCATCTACCGCGCCACCCCCGAGTACCTGGCCACCATGGCCGGGGAGCTGGTGGCGGCCGGCGCAAGCCT GGTGGGGGGCTGCTGTGGCACCACGCCGGAGCACATAGCCGCCATCTCCCGCGCCCTGTCGGGGCGGTGCCCTGCCAGTC GGGCCGGTGCGGCCGCAGTCAGGGTCGGTGAGCCGGAGCAGCGCCCCTCCTCTTCCGGCGGATCGTTCCTGGATGCCTGG GGAAGGCGCAAGCTGATCACCGTGGAGCTGGATCCACCCAAGGGGCTGGACTGCAGCCGCATCATCGAGGGGTGCCGGCG TCTCAAGCGGGCAGGAGTGGATGCCATCAACCTGGCGGAAAACCCCCTGGCGCGTCCCCGCATGGGCAACATCGCCCTGG GGAGCCTGATCCAGCAGCAGGTGGGGATCCCGGTGATCATCCACGTCACCGGCCGCGACCGCAACCTGATCGGCATGCAG TCCGACCTGATGGGAGCCAGCCTGCTGGGGATCCGCTCCGTGCTGGCGGTGACCGGCGACCCGGCCACCATGGGGGACCA CGCCGGAGCCACCTCGGTCTTTGACCTGCACTCCCTGACCCTGATCAAGCTGCTGGCCGACCTGAACCGGGGTGTCAATG CCATGGGCAATCCCATCGGAGGCGGCGCCGGTTTCACCATCGGCGCCGCCTTCAACCCCAATGCGCGGGACATGGCCGGC CAGGCCAGCCGGTTGCGCAAGAAGGTGGCCAACGGCGCCATGTTTGCCCAGACCCAACCGATCTACGATGCGGGCCTCTT CTTCCAGGCACTTGAATTGACCGCCGACTGCGCCATTCCCCTGATACCGGGAATCATGCCGCTGGTCAGCCAGCGCAACG CCGACTACCTGCACAATGAGGTGCCGGGTATCTCCATCCCTGAAACGGTGCTGGCGCGCATGTGTGGACTGGAGAAGGAG GCCGGCGTGGCCGAGGGGCTGGCCATCGCCAGGGAGTTCATTGATGCCAGCTTCCATTCCAGCGGCGGCTACTACCTGAT GCCCCCCTTCGGCAAGGTGGAGCTGGCCCTGGATCTGATCGAGTACATTCACACCAAGGAGAGGGAACCGTCATGCGGAG AATCGTGA
Upstream 100 bases:
>100_bases AAGACTCCCTGTGGCGGTTGAATCAGTAAACAGCCCTTATACCCCATCTTGGGGATTGGGGCAACAGTGTATACCCGGGT GAAACGATGAGGCAAACCCC
Downstream 100 bases:
>100_bases AAATAGTTGCCATGCTGTTCTGCGGCCTGCTTATGGCCGCCTGCGTCCCGGACAAGGGGTTCCGCGAGGAATTCGAGTCC AGTGTCAAGGGGTACAACCG
Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein
Products: NA
Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]
Number of amino acids: Translated: 615; Mature: 615
Protein sequence:
>615_residues MNILERLKGEVLTGDGALGTMLYARGVGLDSSFEQLNLTRPALVRELADDYVAAGAQVIETNTFGANYTRLAALGLEQRV WEINLAGARIARQAADQGRDVLVAGSVGPLPRIRGDEAESDQERMAELFRVQCQALAEGGVDLLLLETFSSLTQLAAAVQ AARCTGLTVAASMAFLEGGRSADGATVEEFCAAMERAGADLVGANCGAGPLELVKVVRRLAAVTHKPITAYANSGFPEYH DGRFIYRATPEYLATMAGELVAAGASLVGGCCGTTPEHIAAISRALSGRCPASRAGAAAVRVGEPEQRPSSSGGSFLDAW GRRKLITVELDPPKGLDCSRIIEGCRRLKRAGVDAINLAENPLARPRMGNIALGSLIQQQVGIPVIIHVTGRDRNLIGMQ SDLMGASLLGIRSVLAVTGDPATMGDHAGATSVFDLHSLTLIKLLADLNRGVNAMGNPIGGGAGFTIGAAFNPNARDMAG QASRLRKKVANGAMFAQTQPIYDAGLFFQALELTADCAIPLIPGIMPLVSQRNADYLHNEVPGISIPETVLARMCGLEKE AGVAEGLAIAREFIDASFHSSGGYYLMPPFGKVELALDLIEYIHTKEREPSCGES
Sequences:
>Translated_615_residues MNILERLKGEVLTGDGALGTMLYARGVGLDSSFEQLNLTRPALVRELADDYVAAGAQVIETNTFGANYTRLAALGLEQRV WEINLAGARIARQAADQGRDVLVAGSVGPLPRIRGDEAESDQERMAELFRVQCQALAEGGVDLLLLETFSSLTQLAAAVQ AARCTGLTVAASMAFLEGGRSADGATVEEFCAAMERAGADLVGANCGAGPLELVKVVRRLAAVTHKPITAYANSGFPEYH DGRFIYRATPEYLATMAGELVAAGASLVGGCCGTTPEHIAAISRALSGRCPASRAGAAAVRVGEPEQRPSSSGGSFLDAW GRRKLITVELDPPKGLDCSRIIEGCRRLKRAGVDAINLAENPLARPRMGNIALGSLIQQQVGIPVIIHVTGRDRNLIGMQ SDLMGASLLGIRSVLAVTGDPATMGDHAGATSVFDLHSLTLIKLLADLNRGVNAMGNPIGGGAGFTIGAAFNPNARDMAG QASRLRKKVANGAMFAQTQPIYDAGLFFQALELTADCAIPLIPGIMPLVSQRNADYLHNEVPGISIPETVLARMCGLEKE AGVAEGLAIAREFIDASFHSSGGYYLMPPFGKVELALDLIEYIHTKEREPSCGES >Mature_615_residues MNILERLKGEVLTGDGALGTMLYARGVGLDSSFEQLNLTRPALVRELADDYVAAGAQVIETNTFGANYTRLAALGLEQRV WEINLAGARIARQAADQGRDVLVAGSVGPLPRIRGDEAESDQERMAELFRVQCQALAEGGVDLLLLETFSSLTQLAAAVQ AARCTGLTVAASMAFLEGGRSADGATVEEFCAAMERAGADLVGANCGAGPLELVKVVRRLAAVTHKPITAYANSGFPEYH DGRFIYRATPEYLATMAGELVAAGASLVGGCCGTTPEHIAAISRALSGRCPASRAGAAAVRVGEPEQRPSSSGGSFLDAW GRRKLITVELDPPKGLDCSRIIEGCRRLKRAGVDAINLAENPLARPRMGNIALGSLIQQQVGIPVIIHVTGRDRNLIGMQ SDLMGASLLGIRSVLAVTGDPATMGDHAGATSVFDLHSLTLIKLLADLNRGVNAMGNPIGGGAGFTIGAAFNPNARDMAG QASRLRKKVANGAMFAQTQPIYDAGLFFQALELTADCAIPLIPGIMPLVSQRNADYLHNEVPGISIPETVLARMCGLEKE AGVAEGLAIAREFIDASFHSSGGYYLMPPFGKVELALDLIEYIHTKEREPSCGES
Specific function: Terminal step in the de novo biosynthesis of methionine. [C]
COG id: COG0685
COG function: function code E; 5,10-methylenetetrahydrofolate reductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Hcy-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=321, Percent_Identity=27.7258566978193, Blast_Score=121, Evalue=2e-27, Organism=Homo sapiens, GI13162290, Length=306, Percent_Identity=32.0261437908497, Blast_Score=107, Evalue=3e-23, Organism=Homo sapiens, GI157266337, Length=308, Percent_Identity=32.1428571428571, Blast_Score=107, Evalue=4e-23, Organism=Escherichia coli, GI1790450, Length=323, Percent_Identity=30.9597523219814, Blast_Score=122, Evalue=6e-29, Organism=Escherichia coli, GI1786456, Length=302, Percent_Identity=32.4503311258278, Blast_Score=88, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17535405, Length=321, Percent_Identity=30.5295950155763, Blast_Score=127, Evalue=2e-29,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003171 - InterPro: IPR003726 [H]
Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]
EC number: =2.1.1.10; =1.5.1.20 [H]
Molecular weight: Translated: 64693; Mature: 64693
Theoretical pI: Translated: 5.41; Mature: 5.41
Prosite motif: PS50970 HCY
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.0 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 2.0 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNILERLKGEVLTGDGALGTMLYARGVGLDSSFEQLNLTRPALVRELADDYVAAGAQVIE CCHHHHHCCCEEECCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEE TNTFGANYTRLAALGLEQRVWEINLAGARIARQAADQGRDVLVAGSVGPLPRIRGDEAES ECCCCCCHHHHHHHHHHHHEEEEECHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC DQERMAELFRVQCQALAEGGVDLLLLETFSSLTQLAAAVQAARCTGLTVAASMAFLEGGR HHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC SADGATVEEFCAAMERAGADLVGANCGAGPLELVKVVRRLAAVTHKPITAYANSGFPEYH CCCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC DGRFIYRATPEYLATMAGELVAAGASLVGGCCGTTPEHIAAISRALSGRCPASRAGAAAV CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE RVGEPEQRPSSSGGSFLDAWGRRKLITVELDPPKGLDCSRIIEGCRRLKRAGVDAINLAE ECCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCC NPLARPRMGNIALGSLIQQQVGIPVIIHVTGRDRNLIGMQSDLMGASLLGIRSVLAVTGD CCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHCCCC PATMGDHAGATSVFDLHSLTLIKLLADLNRGVNAMGNPIGGGAGFTIGAAFNPNARDMAG CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCCCHHHHHH QASRLRKKVANGAMFAQTQPIYDAGLFFQALELTADCAIPLIPGIMPLVSQRNADYLHNE HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHC VPGISIPETVLARMCGLEKEAGVAEGLAIAREFIDASFHSSGGYYLMPPFGKVELALDLI CCCCCCCHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH EYIHTKEREPSCGES HHHHCCCCCCCCCCC >Mature Secondary Structure MNILERLKGEVLTGDGALGTMLYARGVGLDSSFEQLNLTRPALVRELADDYVAAGAQVIE CCHHHHHCCCEEECCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEE TNTFGANYTRLAALGLEQRVWEINLAGARIARQAADQGRDVLVAGSVGPLPRIRGDEAES ECCCCCCHHHHHHHHHHHHEEEEECHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC DQERMAELFRVQCQALAEGGVDLLLLETFSSLTQLAAAVQAARCTGLTVAASMAFLEGGR HHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC SADGATVEEFCAAMERAGADLVGANCGAGPLELVKVVRRLAAVTHKPITAYANSGFPEYH CCCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC DGRFIYRATPEYLATMAGELVAAGASLVGGCCGTTPEHIAAISRALSGRCPASRAGAAAV CCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE RVGEPEQRPSSSGGSFLDAWGRRKLITVELDPPKGLDCSRIIEGCRRLKRAGVDAINLAE ECCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCC NPLARPRMGNIALGSLIQQQVGIPVIIHVTGRDRNLIGMQSDLMGASLLGIRSVLAVTGD CCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHCCCC PATMGDHAGATSVFDLHSLTLIKLLADLNRGVNAMGNPIGGGAGFTIGAAFNPNARDMAG CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCCCHHHHHH QASRLRKKVANGAMFAQTQPIYDAGLFFQALELTADCAIPLIPGIMPLVSQRNADYLHNE HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHC VPGISIPETVLARMCGLEKEAGVAEGLAIAREFIDASFHSSGGYYLMPPFGKVELALDLI CCCCCCCHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH EYIHTKEREPSCGES HHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9353932; 9384377 [H]