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The map label for this gene is nucA [H]

Identifier: 118578619

GI number: 118578619

Start: 179738

End: 180544

Strand: Direct

Name: nucA [H]

Synonym: Ppro_0175

Alternate gene names: 118578619

Gene position: 179738-180544 (Clockwise)

Preceding gene: 118578617

Following gene: 118578620

Centisome position: 4.48

GC content: 50.81

Gene sequence:

>807_bases
ATGAGCATAAAAAGATTTTTTCTTGTAGTATTGTCAATATCCGGTCTTCTGCTGTCCGTCACAGGAATGGCCTCCCAAAC
AGGGTGCCCAGAACATTTCGCCAACGGCATAGCCCCTGATTTCATCAATCAAAAACTCACCGCCAAAACCCGTGAGGTCT
GTTATTCCGGTTTTGCCCTCAAGCATTCCGGTATTACCAGGACTCCCCTTTATGCTGCCGAGCATCTAACGCGTGACCGT
CTAATGCAGGGCAAAGGCCTGAAAAGGCAAAGCCAGTTTCACCCTGACGATAACATTCCACGAACGGAACGATCAGAACT
TCGCCACTATTCCAGGTCTGGCTACGACCGCGGCCATGTGGCACCATCTGCCGACATGTTCGACCTGCAGTCCCAATTTG
AATGCTTCTCGCTGGCGAATATGATCCCCCAAGTTCCGGAAAACAACCGGGGCCCATGGGAAGGGATCGAATCGGCTGTG
AGAATGATGGCAAAGAGTAAAGGTGACATCTATGTCATCACCGGCCCGATCTATCAAGGCAGTAACATCGAAAAGATCGG
GGGTGCTGTGATGGTTCCCACCAAACTGTTCAAGGCAGTTTACGATCCGCAGAGGCGCGAGGCCGGTGCCTACCTGATTG
ACAACATCGCAGATGCCCAGCCTGAGAAAATCTCCATAACCGAGCTAGAAAAAATTACCGGGATCAGCATTTTCCCGTCA
GTAGAGAAAAACGTCAAATCCAGACTCATGCGTCTGCCTGAACCCAAATCATACAAGGAGCGCAAACGCAAGGGAGGACG
AGGATGA

Upstream 100 bases:

>100_bases
CCCCCTCCTGAATATTGCTCTTTCCGTGTTCTCTCCTGCTCTCTCCTTGTAAAATCACCCGCAAATTGATAGAGTTACGC
CTTCTTTTGGAGGTTTCCAT

Downstream 100 bases:

>100_bases
GCAGAAAAGGAATCAAACCCTTCGCAAATGAAAGCGATTGCTTGCAGATTGGCGGCATCACCATCGAAAACCGGGTCGAC
AGGGTGTCGATCTTTGGCAG

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSIKRFFLVVLSISGLLLSVTGMASQTGCPEHFANGIAPDFINQKLTAKTREVCYSGFALKHSGITRTPLYAAEHLTRDR
LMQGKGLKRQSQFHPDDNIPRTERSELRHYSRSGYDRGHVAPSADMFDLQSQFECFSLANMIPQVPENNRGPWEGIESAV
RMMAKSKGDIYVITGPIYQGSNIEKIGGAVMVPTKLFKAVYDPQRREAGAYLIDNIADAQPEKISITELEKITGISIFPS
VEKNVKSRLMRLPEPKSYKERKRKGGRG

Sequences:

>Translated_268_residues
MSIKRFFLVVLSISGLLLSVTGMASQTGCPEHFANGIAPDFINQKLTAKTREVCYSGFALKHSGITRTPLYAAEHLTRDR
LMQGKGLKRQSQFHPDDNIPRTERSELRHYSRSGYDRGHVAPSADMFDLQSQFECFSLANMIPQVPENNRGPWEGIESAV
RMMAKSKGDIYVITGPIYQGSNIEKIGGAVMVPTKLFKAVYDPQRREAGAYLIDNIADAQPEKISITELEKITGISIFPS
VEKNVKSRLMRLPEPKSYKERKRKGGRG
>Mature_267_residues
SIKRFFLVVLSISGLLLSVTGMASQTGCPEHFANGIAPDFINQKLTAKTREVCYSGFALKHSGITRTPLYAAEHLTRDRL
MQGKGLKRQSQFHPDDNIPRTERSELRHYSRSGYDRGHVAPSADMFDLQSQFECFSLANMIPQVPENNRGPWEGIESAVR
MMAKSKGDIYVITGPIYQGSNIEKIGGAVMVPTKLFKAVYDPQRREAGAYLIDNIADAQPEKISITELEKITGISIFPSV
EKNVKSRLMRLPEPKSYKERKRKGGRG

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=223, Percent_Identity=30.9417040358744, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI224451075, Length=189, Percent_Identity=31.2169312169312, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI53759134, Length=212, Percent_Identity=29.7169811320755, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17505967, Length=209, Percent_Identity=32.0574162679426, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322253, Length=213, Percent_Identity=32.3943661971831, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581065, Length=212, Percent_Identity=31.1320754716981, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129899, Length=210, Percent_Identity=29.5238095238095, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 29981; Mature: 29850

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKRFFLVVLSISGLLLSVTGMASQTGCPEHFANGIAPDFINQKLTAKTREVCYSGFAL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
KHSGITRTPLYAAEHLTRDRLMQGKGLKRQSQFHPDDNIPRTERSELRHYSRSGYDRGHV
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
APSADMFDLQSQFECFSLANMIPQVPENNRGPWEGIESAVRMMAKSKGDIYVITGPIYQG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEECC
SNIEKIGGAVMVPTKLFKAVYDPQRREAGAYLIDNIADAQPEKISITELEKITGISIFPS
CCHHHHCCEEEHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCEEEHHHHHHHCCCEECCH
VEKNVKSRLMRLPEPKSYKERKRKGGRG
HHHHHHHHHHCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
SIKRFFLVVLSISGLLLSVTGMASQTGCPEHFANGIAPDFINQKLTAKTREVCYSGFAL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
KHSGITRTPLYAAEHLTRDRLMQGKGLKRQSQFHPDDNIPRTERSELRHYSRSGYDRGHV
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
APSADMFDLQSQFECFSLANMIPQVPENNRGPWEGIESAVRMMAKSKGDIYVITGPIYQG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEECC
SNIEKIGGAVMVPTKLFKAVYDPQRREAGAYLIDNIADAQPEKISITELEKITGISIFPS
CCHHHHCCEEEHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCEEEHHHHHHHCCCEECCH
VEKNVKSRLMRLPEPKSYKERKRKGGRG
HHHHHHHHHHCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]