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The map label for this gene is vanS [H]

Identifier: 118479969

GI number: 118479969

Start: 4636359

End: 4637465

Strand: Reverse

Name: vanS [H]

Synonym: BALH_4415

Alternate gene names: 118479969

Gene position: 4637465-4636359 (Counterclockwise)

Preceding gene: 118479970

Following gene: 118479968

Centisome position: 88.21

GC content: 35.32

Gene sequence:

>1107_bases
ATGGCTAAAAAGATGAGAAGCTTTCGCTCGAAGATGGTTATGCTATTTGCGTTAAGTATGATATTTGCTGGTGGCGTAAC
GTATATCATTTATCAAGGGTTACGGATTTATTACAAATTAGTCGTTCGTTATGAGGATCCGTTAGCTCAAGTGCGTTTCA
TGATAAAACAAATTGGGGATGTTAATTTCTTTTTAATTATTTTCATCCCGCTATCCATTATGTTTTTCTTCTTCCTTACT
AAACCATACTTAAAATATTTTGATGAGATTTCAAGCGGGATTCATCATTTGGCGAACGGAGATTTTTCAAATGAAGTGCG
CATTTCATCAAACGATGAGTTTGGAGAGATCGCACGTGAAATAAATGTTGCAAGTGAAAAGTTAAAAGAAGCGGTGGAAA
GAGGAGACTTTGCTGAAAGTAGTAAAGATCAGCTCGTTGTAAACTTAGCTCATGATTTAAGAACGCCATTAACATCCGTG
TTAGGGTATTTAGATTTAATTCTTAAAGATGAGAATTTAACAAAAGAACAAATTAAACATTTCTCCACAATTGCGTATAC
GAAATCGCAAAGGCTGGAAAGTTTAATCGATGAGTTATTTGAAATTACACGTATGAATTATGGCATGCTAAAGCTGGATA
AAAAGCCGATTGATATAAGTGAGCTACTTATACAGTTAGAGGAGGAATTGTATCCGTTATTAGAAAAACATCATTTAGAA
GCTAGATTGAATGTTGATCCTCATTTGCCGATTAACGGTGATGGAAAATTGTTAGCTAGAGTATTTGAAAACTTGTTAAC
AAATGCCGTTCGATACGGATATGATGGGCAATTTGTTGATCTGAATGGGTATATCGATAATGGAGAAGTTGTCGTACAAG
TTATGAATTACGGAGATAGCATTCCAGAAGAAGACTTACCGTATCTTTTTGATATGTTTTATACAGGTGATAAATCGAGG
TCAGAGAACCGTGGCGGGACAGGCCTTGGGCTATTTATTGCGAAAAATATTGTTGAACAGCATAATGGTACGATTTCTGC
TGAAAGTAATGTAGTTAGAACAATGTTTGAAGTGCGATTGCCAAAAGACGAGAACGTAACAATTTAA

Upstream 100 bases:

>100_bases
ACAGTTATGGTACATATTCGTACGTTACGGAAAAAACTTGGGGAAGATAAAAGAAAGAACAAGTTAATCAAAACAGTGTG
GGGAGTAGGTTATACTTTCA

Downstream 100 bases:

>100_bases
TAAAAATTTAAACTTTACCCCACTTTTTATTTTAATAGTTTTTTCTATTCTATACGTAAGTTACGTTAAGGAGGAACTAG
AAATGAAAAAGTGGGTATTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MAKKMRSFRSKMVMLFALSMIFAGGVTYIIYQGLRIYYKLVVRYEDPLAQVRFMIKQIGDVNFFLIIFIPLSIMFFFFLT
KPYLKYFDEISSGIHHLANGDFSNEVRISSNDEFGEIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKPIDISELLIQLEEELYPLLEKHHLE
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVMNYGDSIPEEDLPYLFDMFYTGDKSR
SENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTMFEVRLPKDENVTI

Sequences:

>Translated_368_residues
MAKKMRSFRSKMVMLFALSMIFAGGVTYIIYQGLRIYYKLVVRYEDPLAQVRFMIKQIGDVNFFLIIFIPLSIMFFFFLT
KPYLKYFDEISSGIHHLANGDFSNEVRISSNDEFGEIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSV
LGYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKPIDISELLIQLEEELYPLLEKHHLE
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVMNYGDSIPEEDLPYLFDMFYTGDKSR
SENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTMFEVRLPKDENVTI
>Mature_367_residues
AKKMRSFRSKMVMLFALSMIFAGGVTYIIYQGLRIYYKLVVRYEDPLAQVRFMIKQIGDVNFFLIIFIPLSIMFFFFLTK
PYLKYFDEISSGIHHLANGDFSNEVRISSNDEFGEIAREINVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVL
GYLDLILKDENLTKEQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKPIDISELLIQLEEELYPLLEKHHLEA
RLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVMNYGDSIPEEDLPYLFDMFYTGDKSRS
ENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTMFEVRLPKDENVTI

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=30.1818181818182, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789149, Length=268, Percent_Identity=29.4776119402985, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=28.8702928870293, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=29.5652173913043, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87082128, Length=318, Percent_Identity=28.3018867924528, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=28.3261802575107, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788713, Length=295, Percent_Identity=26.7796610169492, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=28.6343612334802, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=28.8793103448276, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790346, Length=212, Percent_Identity=27.8301886792453, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787894, Length=310, Percent_Identity=23.8709677419355, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788279, Length=311, Percent_Identity=26.0450160771704, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081816, Length=370, Percent_Identity=24.3243243243243, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790861, Length=230, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=25.1141552511416, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788549, Length=306, Percent_Identity=25.1633986928105, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42265; Mature: 42134

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKMRSFRSKMVMLFALSMIFAGGVTYIIYQGLRIYYKLVVRYEDPLAQVRFMIKQIGD
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
VNFFLIIFIPLSIMFFFFLTKPYLKYFDEISSGIHHLANGDFSNEVRISSNDEFGEIARE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHH
INVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKEQIKH
HHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKPIDISELLIQLEEELYPLLEKHHLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVMNYGDS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEEECCCCC
IPEEDLPYLFDMFYTGDKSRSENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTMFEVRL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
PKDENVTI
CCCCCCCC
>Mature Secondary Structure 
AKKMRSFRSKMVMLFALSMIFAGGVTYIIYQGLRIYYKLVVRYEDPLAQVRFMIKQIGD
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
VNFFLIIFIPLSIMFFFFLTKPYLKYFDEISSGIHHLANGDFSNEVRISSNDEFGEIARE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHH
INVASEKLKEAVERGDFAESSKDQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKEQIKH
HHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKPIDISELLIQLEEELYPLLEKHHLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ARLNVDPHLPINGDGKLLARVFENLLTNAVRYGYDGQFVDLNGYIDNGEVVVQVMNYGDS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEEECCCCC
IPEEDLPYLFDMFYTGDKSRSENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTMFEVRL
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
PKDENVTI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]