BacMap

Definition	Bacillus thuringiensis str. Al Hakam chromosome, complete genome.
Accession	NC_008600
Length	5,257,091

Click here to switch to the map view.

The map label for this gene is caiD [H]

Identifier: 118478779

GI number: 118478779

Start: 3354442

End: 3355233

Strand: Reverse

Name: caiD [H]

Synonym: BALH_3171

Alternate gene names: 118478779

Gene position: 3355233-3354442 (Counterclockwise)

Preceding gene: 118478780

Following gene: 118478778

Centisome position: 63.82

GC content: 35.98

Gene sequence:

>792_bases
ATGAAAAATGAACGATTAGTAATATACAGTAAAAAAGGGAGTAGTGCAGTCATTACAATTCAAAACCCACCAGTCAACGC
TTTAAGTTTAGAAGTAGTGCAACAATTAATAAATGTATTAGAGGAAATAGAGATGGACGATGATATTGCAGTCGTAATTA
TAACTGGAATTGGGGAGAAAGCCTTTGTAGCTGGAGGGGATATAAAAGAATTTCCAGATTGGATAGGAAAAGGTGAAAAA
TATGCTGAGATGAAATCAATAGAATTACAAAAGCCTCTTAATCAACTAGAGAATTTGTCTAAACCAACTATAGCTGCTAT
TAATGGGTTAGCACTAGGCGGAGGTTGTGAATTAGCACTTGCTTGTGATTTAAGAGTAATAGAAGAACAAGCCTTAATTG
GATTACCAGAAATTACGTTAGGTCTATTTCCTGGGGCTGGAGGAACTCAGCGCTTACCTAGATTAATTGGAGAAGGAAAA
GCAAAAGAGATGATGTTCACAGGAAAACCAATTACAGCGAAAGAAGCAAAAGAAATAAATTTAGTTAACTATATTACCTC
TCGAGGAGAGGCGTTAAATAAAGCAAAAGAGATTGCTAAAGATATAAGTGAATTTTCTCTTCCAGCACTTTCCTATATGA
AGTTAGCAATTCATGAAGGTTTGGCAGTACCTTTACAAGAAGGGCTTCAGATCGAAGCGCGTTATTTTGGGAAAGTGTTT
CAAACAGAAGATGTGAAGGAGGGGGTAAAGGCATTTATTGAAAAAAGAGTACCGCGTTTCACCAATAAATAA

Upstream 100 bases:

>100_bases
TTTCTAAAAAGGGAAATATATTCACTAATTCAGGCTAACAGTGAGTAAATCGAAGAAACTTGAACTTGAGAAAAATTAGG
GATTTAAAAGGGGACTTTTT

Downstream 100 bases:

>100_bases
AAAGTATTGGGGTGTATTTTACAATTGCCCACACAGATGCTACTTTTAAAGCTATTTATTTGATTCTTAAAATATCTTTC
AGAGCTGTTTCATCTAAGAA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNERLVIYSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGEKAFVAGGDIKEFPDWIGKGEK
YAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIHEGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK

Sequences:

>Translated_263_residues
MKNERLVIYSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGEKAFVAGGDIKEFPDWIGKGEK
YAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIHEGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK
>Mature_263_residues
MKNERLVIYSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGEKAFVAGGDIKEFPDWIGKGEK
YAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIHEGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=36.2264150943396, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=39.0243902439024, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=36.1256544502618, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI20127408, Length=181, Percent_Identity=38.6740331491713, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=29.6, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI150378541, Length=242, Percent_Identity=30.9917355371901, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI37594469, Length=153, Percent_Identity=37.9084967320261, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI37594471, Length=153, Percent_Identity=37.9084967320261, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI70995211, Length=241, Percent_Identity=29.8755186721992, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757968, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI49355787, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=22.8571428571429, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4757966, Length=227, Percent_Identity=23.3480176211454, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI51538581, Length=227, Percent_Identity=23.3480176211454, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=41.0526315789474, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI62530384, Length=186, Percent_Identity=29.5698924731183, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI221136756, Length=236, Percent_Identity=23.3050847457627, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=236, Percent_Identity=23.3050847457627, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=26.2008733624454, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=26.2008733624454, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=22.1789883268482, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=202, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI45643119, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI260275230, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=191, Percent_Identity=26.7015706806283, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI295842266, Length=128, Percent_Identity=32.03125, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI157694520, Length=155, Percent_Identity=27.741935483871, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=266, Percent_Identity=38.7218045112782, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=33.4600760456274, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=32, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=39.1959798994975, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=35.7954545454545, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=243, Percent_Identity=27.5720164609054, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=30.8, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=38.1889763779528, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=36.7816091954023, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=34.5454545454545, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=37.3626373626374, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=209, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=165, Percent_Identity=36.969696969697, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=165, Percent_Identity=36.969696969697, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=165, Percent_Identity=36.969696969697, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=232, Percent_Identity=31.0344827586207, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=28.4584980237154, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=25.2918287937743, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=27.7272727272727, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=178, Percent_Identity=29.2134831460674, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=40.3225806451613, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=40.3225806451613, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=38.4920634920635, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI21357171, Length=264, Percent_Identity=31.0606060606061, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24650670, Length=232, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=27.1255060728745, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24654903, Length=247, Percent_Identity=23.4817813765182, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571729, Length=154, Percent_Identity=33.1168831168831, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571730, Length=154, Percent_Identity=33.1168831168831, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921000, Length=173, Percent_Identity=36.4161849710983, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583077, Length=173, Percent_Identity=36.4161849710983, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=173, Percent_Identity=36.4161849710983, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921018, Length=187, Percent_Identity=33.1550802139037, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583165, Length=242, Percent_Identity=28.099173553719, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550169, Length=187, Percent_Identity=31.0160427807487, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28693; Mature: 28693

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNERLVIYSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGEK
CCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
AFVAGGDIKEFPDWIGKGEKYAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELAL
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCCCCEEE
ACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKPITAKEAKEIN
EECHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
LVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIHEGLAVPLQEGLQIEARYFGKVF
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHH
QTEDVKEGVKAFIEKRVPRFTNK
CCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNERLVIYSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGEK
CCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
AFVAGGDIKEFPDWIGKGEKYAEMKSIELQKPLNQLENLSKPTIAAINGLALGGGCELAL
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCCCCEEE
ACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKPITAKEAKEIN
EECHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
LVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIHEGLAVPLQEGLQIEARYFGKVF
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHH
QTEDVKEGVKAFIEKRVPRFTNK
CCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]