Click here to switch to the map view.

The map label for this gene is ydaB [H]

Identifier: 118478688

GI number: 118478688

Start: 3257668

End: 3258966

Strand: Reverse

Name: ydaB [H]

Synonym: BALH_3074

Alternate gene names: 118478688

Gene position: 3258966-3257668 (Counterclockwise)

Preceding gene: 118478689

Following gene: 118478687

Centisome position: 61.99

GC content: 35.8

Gene sequence:

>1299_bases
ATGATGGCCAGTTTAAAAATTGGAGCGGTATGTATTCCGCTAAATCATCAGCTTACTGTTTATGAGCTCGAAACGATTGT
AAAAGAAGCGAAATTAAAAGTGTTAGTTATTGATGAAGAATTTAGTGAAGTGTTATTAAAGGTTGATGCTGTTAAAGAAA
TTCCTTATGTAATTCAAACGACAAAAGAAGGCTTTGGTTCATTTGAATTAGAGTTGCAAAAACAGCCAATGACAGAACCG
AACGTAGAAGTTCATGAAGAAGACGATGCCATTTACTTATTTACTTCAGGAACGACTGGACAGGCAAAAGCATGTGTAAT
TGGTCATAAAAACTTACATCACTATTTTGCAGAGATTGCAGGACAAAGAGAAATTCCAGCAGGTGAACGCTTTTTATCCG
TGCATCCATTATTTCATATGAGTGGCGTGCTTTCTATTTTAAATTGTATTTATCATGGTGTTACGATGATCTTCTTAGCT
GATTCGAATCCTACTCTTATTTGGGATAAGATTGAAGAAGAGAAAATTACTACGATGCTTGCATTCCCAGCTGTTTATAG
CTATATGCTTGATGAACTAAATAAAAAAGAACGTAACATTTCTACTTTTAAAGTAGCACAAAGCGGTGGAACAAAAGTGC
CAGAAACACTCATCCAAAAATATATGGAAAAAGGAATCTATATGGTGCAAGGCTATGGTAGTACAGAAGGTTGGGTTGTA
ACATCATGGCATCCAATGATGGGAAAAGAAAAGATGTCTTCTGTAGGAAAAACCTTGAAAAATGTAGAGGTGAAAATTGT
TCATCCAGAAACAGGTGATGAACTAACAACAAATGAAGTGGGAGAGATTCATGTAAGAAGTCCATACATGTTTAAAGGGT
ATTGGAATAATGAAAAGGCGACAAAGAAGGTAGTAAAAGATAATTGGTTTAACATGGGTGATGCAGGTATGATAGATGAC
GACGGATTCCTGCATATTATGGGAAGATATAAAGACGTTATTATACGTGGTGGTGACAACGTATATCCAGACCAAGTAGA
AGATGTTATTCATGAAATACATGGTGTTTTAGAAGTTGCAGTAGTTGGAGTGCCAGATGACTTTTGGGGTGAGGTTCCAA
GAGCTTACATTGTGAAGGATGGCGAAACAACAGTAACAGAAGAAAGTATTATTCAGTATTGTAAGGAGAAGTTAGCAAGT
TACAAAATTCCAGAAGTGGTATTCGTAGAAGAGTTACCGAAAAACGCTTTAGGAAAAGTGTTGAAGAGAGAATTGAGAGA
AAATGTACTTGTAAAATAA

Upstream 100 bases:

>100_bases
AACGAGTAAATCAGCTTGCACATTATTTGTTACATAGTGGTGTGCAAAGAGGGGATCGTATAGGATACTATGCAAAAATA
ATCATCCGTTTCCAAGCGTT

Downstream 100 bases:

>100_bases
AAAACGAGTAGATAATGAGGAATCCAGTTATGTACAAAGCATAACTGGATTTTTTGTTTATGGTTCTGCTGATGGGGATT
GTGAAAATTCAAAACTCTTT

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MMASLKIGAVCIPLNHQLTVYELETIVKEAKLKVLVIDEEFSEVLLKVDAVKEIPYVIQTTKEGFGSFELELQKQPMTEP
NVEVHEEDDAIYLFTSGTTGQAKACVIGHKNLHHYFAEIAGQREIPAGERFLSVHPLFHMSGVLSILNCIYHGVTMIFLA
DSNPTLIWDKIEEEKITTMLAFPAVYSYMLDELNKKERNISTFKVAQSGGTKVPETLIQKYMEKGIYMVQGYGSTEGWVV
TSWHPMMGKEKMSSVGKTLKNVEVKIVHPETGDELTTNEVGEIHVRSPYMFKGYWNNEKATKKVVKDNWFNMGDAGMIDD
DGFLHIMGRYKDVIIRGGDNVYPDQVEDVIHEIHGVLEVAVVGVPDDFWGEVPRAYIVKDGETTVTEESIIQYCKEKLAS
YKIPEVVFVEELPKNALGKVLKRELRENVLVK

Sequences:

>Translated_432_residues
MMASLKIGAVCIPLNHQLTVYELETIVKEAKLKVLVIDEEFSEVLLKVDAVKEIPYVIQTTKEGFGSFELELQKQPMTEP
NVEVHEEDDAIYLFTSGTTGQAKACVIGHKNLHHYFAEIAGQREIPAGERFLSVHPLFHMSGVLSILNCIYHGVTMIFLA
DSNPTLIWDKIEEEKITTMLAFPAVYSYMLDELNKKERNISTFKVAQSGGTKVPETLIQKYMEKGIYMVQGYGSTEGWVV
TSWHPMMGKEKMSSVGKTLKNVEVKIVHPETGDELTTNEVGEIHVRSPYMFKGYWNNEKATKKVVKDNWFNMGDAGMIDD
DGFLHIMGRYKDVIIRGGDNVYPDQVEDVIHEIHGVLEVAVVGVPDDFWGEVPRAYIVKDGETTVTEESIIQYCKEKLAS
YKIPEVVFVEELPKNALGKVLKRELRENVLVK
>Mature_432_residues
MMASLKIGAVCIPLNHQLTVYELETIVKEAKLKVLVIDEEFSEVLLKVDAVKEIPYVIQTTKEGFGSFELELQKQPMTEP
NVEVHEEDDAIYLFTSGTTGQAKACVIGHKNLHHYFAEIAGQREIPAGERFLSVHPLFHMSGVLSILNCIYHGVTMIFLA
DSNPTLIWDKIEEEKITTMLAFPAVYSYMLDELNKKERNISTFKVAQSGGTKVPETLIQKYMEKGIYMVQGYGSTEGWVV
TSWHPMMGKEKMSSVGKTLKNVEVKIVHPETGDELTTNEVGEIHVRSPYMFKGYWNNEKATKKVVKDNWFNMGDAGMIDD
DGFLHIMGRYKDVIIRGGDNVYPDQVEDVIHEIHGVLEVAVVGVPDDFWGEVPRAYIVKDGETTVTEESIIQYCKEKLAS
YKIPEVVFVEELPKNALGKVLKRELRENVLVK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=471, Percent_Identity=31.2101910828025, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI187761345, Length=463, Percent_Identity=27.645788336933, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI187761343, Length=463, Percent_Identity=27.645788336933, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI38505220, Length=456, Percent_Identity=28.2894736842105, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI122937307, Length=453, Percent_Identity=27.5938189845475, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI42544132, Length=450, Percent_Identity=27.3333333333333, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI58082049, Length=463, Percent_Identity=25.7019438444924, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI157311624, Length=463, Percent_Identity=24.8380129589633, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI157311622, Length=463, Percent_Identity=24.8380129589633, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI115511026, Length=456, Percent_Identity=25.219298245614, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI13325057, Length=350, Percent_Identity=25.4285714285714, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI227499619, Length=453, Percent_Identity=25.1655629139073, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI28416953, Length=472, Percent_Identity=24.1525423728814, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI13375727, Length=184, Percent_Identity=29.3478260869565, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI27477105, Length=135, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788107, Length=354, Percent_Identity=30.5084745762712, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI145693145, Length=459, Percent_Identity=27.0152505446623, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI221142682, Length=438, Percent_Identity=24.4292237442922, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786810, Length=347, Percent_Identity=27.3775216138329, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788595, Length=346, Percent_Identity=29.4797687861272, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=23.8888888888889, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=441, Percent_Identity=31.0657596371882, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17559526, Length=440, Percent_Identity=29.3181818181818, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI32563687, Length=441, Percent_Identity=31.0657596371882, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17560308, Length=352, Percent_Identity=33.5227272727273, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI71994690, Length=360, Percent_Identity=32.5, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71994694, Length=360, Percent_Identity=32.5, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71994703, Length=360, Percent_Identity=32.5, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17538037, Length=452, Percent_Identity=27.4336283185841, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17557194, Length=454, Percent_Identity=27.5330396475771, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=455, Percent_Identity=26.8131868131868, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71996755, Length=445, Percent_Identity=27.1910112359551, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71985884, Length=357, Percent_Identity=28.2913165266106, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560140, Length=365, Percent_Identity=28.2191780821918, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25141359, Length=432, Percent_Identity=25.2314814814815, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=471, Percent_Identity=22.2929936305732, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17510401, Length=413, Percent_Identity=23.728813559322, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=441, Percent_Identity=28.3446712018141, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319264, Length=381, Percent_Identity=23.6220472440945, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=498, Percent_Identity=25.1004016064257, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355181, Length=447, Percent_Identity=27.7404921700224, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18859661, Length=355, Percent_Identity=30.9859154929577, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24581924, Length=345, Percent_Identity=31.0144927536232, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648260, Length=348, Percent_Identity=31.0344827586207, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356947, Length=347, Percent_Identity=29.1066282420749, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24653035, Length=342, Percent_Identity=27.1929824561404, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130357, Length=402, Percent_Identity=26.865671641791, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648253, Length=339, Percent_Identity=27.1386430678466, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24656500, Length=355, Percent_Identity=27.6056338028169, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648255, Length=339, Percent_Identity=27.1386430678466, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922652, Length=452, Percent_Identity=24.5575221238938, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356441, Length=344, Percent_Identity=27.906976744186, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161076582, Length=354, Percent_Identity=26.8361581920904, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21358303, Length=313, Percent_Identity=25.8785942492013, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281365686, Length=314, Percent_Identity=25.4777070063694, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648257, Length=353, Percent_Identity=25.7790368271955, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921316, Length=441, Percent_Identity=24.2630385487528, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17933690, Length=354, Percent_Identity=24.2937853107345, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 48826; Mature: 48826

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMASLKIGAVCIPLNHQLTVYELETIVKEAKLKVLVIDEEFSEVLLKVDAVKEIPYVIQT
CCCCEEECEEEEECCCCEEEHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCHHEEE
TKEGFGSFELELQKQPMTEPNVEVHEEDDAIYLFTSGTTGQAKACVIGHKNLHHYFAEIA
CCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECHHHHHHHHHHC
GQREIPAGERFLSVHPLFHMSGVLSILNCIYHGVTMIFLADSNPTLIWDKIEEEKITTML
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCHHHHHHHH
AFPAVYSYMLDELNKKERNISTFKVAQSGGTKVPETLIQKYMEKGIYMVQGYGSTEGWVV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCCEEE
TSWHPMMGKEKMSSVGKTLKNVEVKIVHPETGDELTTNEVGEIHVRSPYMFKGYWNNEKA
EECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECCCEEEEEEECCCHH
TKKVVKDNWFNMGDAGMIDDDGFLHIMGRYKDVIIRGGDNVYPDQVEDVIHEIHGVLEVA
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHEEEE
VVGVPDDFWGEVPRAYIVKDGETTVTEESIIQYCKEKLASYKIPEVVFVEELPKNALGKV
EECCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCHHHHHHH
LKRELRENVLVK
HHHHHHHHCCCC
>Mature Secondary Structure
MMASLKIGAVCIPLNHQLTVYELETIVKEAKLKVLVIDEEFSEVLLKVDAVKEIPYVIQT
CCCCEEECEEEEECCCCEEEHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCHHEEE
TKEGFGSFELELQKQPMTEPNVEVHEEDDAIYLFTSGTTGQAKACVIGHKNLHHYFAEIA
CCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECHHHHHHHHHHC
GQREIPAGERFLSVHPLFHMSGVLSILNCIYHGVTMIFLADSNPTLIWDKIEEEKITTML
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCHHHHHHHH
AFPAVYSYMLDELNKKERNISTFKVAQSGGTKVPETLIQKYMEKGIYMVQGYGSTEGWVV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCCEEE
TSWHPMMGKEKMSSVGKTLKNVEVKIVHPETGDELTTNEVGEIHVRSPYMFKGYWNNEKA
EECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECCCEEEEEEECCCHH
TKKVVKDNWFNMGDAGMIDDDGFLHIMGRYKDVIIRGGDNVYPDQVEDVIHEIHGVLEVA
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHEEEE
VVGVPDDFWGEVPRAYIVKDGETTVTEESIIQYCKEKLASYKIPEVVFVEELPKNALGKV
EECCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCHHHHHHH
LKRELRENVLVK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]