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The map label for this gene is 118478362

Identifier: 118478362

GI number: 118478362

Start: 2892296

End: 2893321

Strand: Reverse

Name: 118478362

Synonym: BALH_2726

Alternate gene names: NA

Gene position: 2893321-2892296 (Counterclockwise)

Preceding gene: 118478363

Following gene: 118478361

Centisome position: 55.04

GC content: 37.52

Gene sequence:

>1026_bases
ATGAAAATTCTAATACTAGGTGGTACACGATTTTTAGGAAGAGCATTTGTAGAAAAGGCTTTGAACAGAGGGTATGAAGT
TACATTATTTAACCGCGGAACAAACAAAGAAATTTTTCCTGAAGTGGAGCAGCTTATCGGTGACAGAAATGGTGATGTAT
CAAGTTTAGAAAATCGAAAATGGGATGTTGTCGTAGATACATGTGGGTTTTCTCCACATCACATTAGAAATGTTGGAGAA
GTGCTTAAAGATAATATTGAACACTATATATTCATCTCAAGCCTTTCCGTATATAAAGATTGGATTCCGCATCATATAAA
AGAAGACTATATATTACAACCTGAACCGACGGCTGAGCAAATAAAGGCTGTAGAAAATGGTGAAGTATCTCCTTATGAGT
ATTACGGTGCGCTGAAAGTATTGTGTGAAAAAGAAGCAGAGAAGTATTGGCCGGGGCGTGTTTTACACGTAAGAGCAGGA
CTTCTTTCAGGGATGTTTGATTATACAGATCGGCTTCCATACTGGATTGGTCGTGTAGCAAAGGGAGGAGAAGTGTTAGT
TCCAGGAAGAAAAGACCGTCCAGTGCAGATAGTTGATATAAAAGACGTCGCAAATTGGGGACTAAATATGGCAGAAAACA
ATAAAGCAGGTATATTCAATGTTACAGGTCCAAATTATGATTTGACGATGGAAGAACTATTAAATACGTGTAAAAAGGTT
ACGAATAGTGATGCTGAATTCGTTTGGATAGAGGAATCGTTCATGAATGAACATAAAGTGCAGCCTTGGACAGAAATGCC
TTTATGGATTCCAGAAACTTTTTCATTAGAAGGGGAAACGAAGCCGTGGAAAGGTGGATTTTCTATTAGTATCAATAGTG
CTATTAAAGCAGGGCTTACTTTTAGAAGGTTAGAAGATACAGTTATGGATGTGTATGTGTGGATGAAGAGTATAGAGGAA
TGGGAATTAAAAGCAGGGATTTCAGGCGAAAGGGAGAAAGAATTGTTAGAAAAATGGTATCAATAA

Upstream 100 bases:

>100_bases
ACTAAAATTATAGTGAAAATCTGCATCCCACCCGTAACTTTCCGTAGCAAAAAATCGTTTAACAAAAGAAAGGGTTTTTA
ATGATGAATGGGGGAGAACC

Downstream 100 bases:

>100_bases
ATTTAGAGGAGCTCTTTGAATATAGTTGATTGCACATAAGGAGGAGAAAAGGTTATGTGGAAAGAAACCATTCATAGTAT
TTCATCAAACTTAAGCTTAA

Product: isoflavone reductase

Products: 2'-hydroxyformononetin; NADPH; H+

Alternate protein names: Reductase; Isoflavone Reductase; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; Oxidoreductase; DTDP-Glucose 4 6-Dehydratase; Nucleotide Sugar Epimerase; NmrA Family Protein; Sugar Dehydratase; Dtdpglucose 4 6-Dehydratase-Like Protein; NAD-Dependent Protein; NAD Dependent Epimerase; NAD Dependent Epimerase/Dehydratase Family

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKILILGGTRFLGRAFVEKALNRGYEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRKWDVVVDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTAEQIKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGEVLVPGRKDRPVQIVDIKDVANWGLNMAENNKAGIFNVTGPNYDLTMEELLNTCKKV
TNSDAEFVWIEESFMNEHKVQPWTEMPLWIPETFSLEGETKPWKGGFSISINSAIKAGLTFRRLEDTVMDVYVWMKSIEE
WELKAGISGEREKELLEKWYQ

Sequences:

>Translated_341_residues
MKILILGGTRFLGRAFVEKALNRGYEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRKWDVVVDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTAEQIKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGEVLVPGRKDRPVQIVDIKDVANWGLNMAENNKAGIFNVTGPNYDLTMEELLNTCKKV
TNSDAEFVWIEESFMNEHKVQPWTEMPLWIPETFSLEGETKPWKGGFSISINSAIKAGLTFRRLEDTVMDVYVWMKSIEE
WELKAGISGEREKELLEKWYQ
>Mature_341_residues
MKILILGGTRFLGRAFVEKALNRGYEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRKWDVVVDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTAEQIKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGEVLVPGRKDRPVQIVDIKDVANWGLNMAENNKAGIFNVTGPNYDLTMEELLNTCKKV
TNSDAEFVWIEESFMNEHKVQPWTEMPLWIPETFSLEGETKPWKGGFSISINSAIKAGLTFRRLEDTVMDVYVWMKSIEE
WELKAGISGEREKELLEKWYQ

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.45

Molecular weight: Translated: 39271; Mature: 39271

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILILGGTRFLGRAFVEKALNRGYEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRK
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCC
WDVVVDTCGFSPHHIRNVGEVLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTAEQ
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCEEECCCCCHHH
IKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAGLLSGMFDYTDRLPYWIGRVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHC
KGGEVLVPGRKDRPVQIVDIKDVANWGLNMAENNKAGIFNVTGPNYDLTMEELLNTCKKV
CCCEEEECCCCCCCEEEEEEHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
TNSDAEFVWIEESFMNEHKVQPWTEMPLWIPETFSLEGETKPWKGGFSISINSAIKAGLT
CCCCCCEEEEEHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEECHHHHHCCH
FRRLEDTVMDVYVWMKSIEEWELKAGISGEREKELLEKWYQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKILILGGTRFLGRAFVEKALNRGYEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRK
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCC
WDVVVDTCGFSPHHIRNVGEVLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTAEQ
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCEEECCCCCHHH
IKAVENGEVSPYEYYGALKVLCEKEAEKYWPGRVLHVRAGLLSGMFDYTDRLPYWIGRVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHC
KGGEVLVPGRKDRPVQIVDIKDVANWGLNMAENNKAGIFNVTGPNYDLTMEELLNTCKKV
CCCEEEECCCCCCCEEEEEEHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
TNSDAEFVWIEESFMNEHKVQPWTEMPLWIPETFSLEGETKPWKGGFSISINSAIKAGLT
CCCCCCEEEEEHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEECHHHHHCCH
FRRLEDTVMDVYVWMKSIEEWELKAGISGEREKELLEKWYQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vestitone; NADP+

Specific reaction: vestitone + NADP+ = 2'-hydroxyformononetin + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA