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The map label for this gene is proB [H]

Identifier: 118478316

GI number: 118478316

Start: 2841722

End: 2842966

Strand: Reverse

Name: proB [H]

Synonym: BALH_2679

Alternate gene names: 118478316

Gene position: 2842966-2841722 (Counterclockwise)

Preceding gene: 118478318

Following gene: 118478315

Centisome position: 54.08

GC content: 40.08

Gene sequence:

>1245_bases
ATGAAGGGCGAAGCGGTCATTTTTATTTTGCTCTTAAATAGACTAGGTGCAGCACTGATAAAAATAGAAATCGCTGCAAG
CTGTATGAATAAAAGTGTAATGTATAAAAAAACTTTGATTTTAGACTTGGGGGATATAAAAGTGAAAAAACAACGAATTG
TTGTAAAGATTGGGAGCAGTTCTTTGGCGGATAGTCACGAAGGCATTTCTAAAGAACAACTTTCAGATCATGTTGCGGCA
TTAGCTCGATTGAAAGAGGAAGGGCATGAAGTTTTGTTAATTACATCTGGGGCCGTTGCTGCAGGTTTTTCTGCGCTAGG
ATATCCTAGCAGACCTGTAACAATTAAAGGAAAACAGGCAGCTGCGGCTGTCGGACAAAGTTTGTTAATGCAGGCGTACA
CGGAGGAATTTCGTAAATACGGTATCGTTACTGCGCAACTATTGCTGACGAGAAGTGATTTTTCTAGAAAAGAACAATAT
AGTAACGCTTACGCTACTTTAGGAGAGTTACTAAACCGTTCTGCTCTTCCAATCATTAATGAAAATGATTCCATTTCTTT
AGAGGAGCTAACGTTCGGTGATAATGATATGCTGTCAGCTTTAGTAAGCGGACTTGTTTTGGCGGATATGCTGATGATTT
TTACAGATGTGAACGGTTTATATGACAAAAATCCTCAGAAAAATGAGGATGCGAAAAAATATTATTTCCTTCCTGAAGTT
ACAGAAGAAATCGCTTCTCTTACAGGAGATGCTGGCTCAAAACTTGGGACTGGCGGAATGAAATCAAAAGTCGATGCTGC
AAAGACAGCACTCTCTCTTGGTGTCAGTGTATTTATCGGAACAGGACGTGGACAAGAGAAATTTGTAGATGTTTTGAAGG
GGAAAGGTGATGGAACGTATGTCGGAAATGCCCCTCAAAAAGAAATGAAGATGAATAAGCAATGGATCGCTTTGCATTCG
GTTGTTAGCGGACAAATTGAAATAGACGCCGGAGCAGCTACAGCTATCATTCAGCATGGCAAGAGTCTTCTTCCTGCTGG
TGTTACAAATGTTTCAGGGGTTTTCCAAGTGGGCGAAGTCGTAGAAGTAATGACAAAGCAAGGACGCGTGATTGGAAAAG
GACAATGCACATATAGTGCAGAGGAGCTAAGGGATGTAAAAGGTATGCAAAGCCAACATATTCAAGCAAGAGGCGAAAGA
CATAATTATGAAGTTATTCATAGAGATTATTGGGTTTCACTTTAA

Upstream 100 bases:

>100_bases
AGTTTAGGCGTTCGCCTGCGTTATAGGCACCAAGTGGATATCTATGTATATCAATTAAGGTGGTACCGCGGAACAATGAC
CGTTTCGTCCTTTTTATTTT

Downstream 100 bases:

>100_bases
GAATAGTGATGTTAAAAAGGAGGAAACAAAAATGAATGAAGTGTTGGCAAAGGGGAAAAAAGCGAAAGAAATTGCTAGAG
AACTTGTACTAAAATCTACA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSSSLADSHEGISKEQLSDHVAA
LARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQY
SNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVLADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
TEEIASLTGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHS
VVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGVFQVGEVVEVMTKQGRVIGKGQCTYSAEELRDVKGMQSQHIQARGER
HNYEVIHRDYWVSL

Sequences:

>Translated_414_residues
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSSSLADSHEGISKEQLSDHVAA
LARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQY
SNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVLADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
TEEIASLTGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHS
VVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGVFQVGEVVEVMTKQGRVIGKGQCTYSAEELRDVKGMQSQHIQARGER
HNYEVIHRDYWVSL
>Mature_414_residues
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSSSLADSHEGISKEQLSDHVAA
LARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQY
SNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVLADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
TEEIASLTGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHS
VVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGVFQVGEVVEVMTKQGRVIGKGQCTYSAEELRDVKGMQSQHIQARGER
HNYEVIHRDYWVSL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=32.5174825174825, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=32.7464788732394, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=38.4615384615385, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17569969, Length=305, Percent_Identity=34.0983606557377, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17569967, Length=284, Percent_Identity=35.5633802816901, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=34.4497607655502, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6321822, Length=401, Percent_Identity=33.6658354114713, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=35.8885017421603, Blast_Score=144, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 44716; Mature: 44716

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSS
CCCCCEEHHHHHHHHCHHHHHHHHHHHHHCCHHEEEEEEEEECCCCEEEEEEEEEEECCC
SLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCCCCCCCEEEECCHH
AAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIIN
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
ENDSISLEELTFGDNDMLSALVSGLVLADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHH
TEEIASLTGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCC
VGNAPQKEMKMNKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGVFQVGEV
CCCCCHHHHHHCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VEVMTKQGRVIGKGQCTYSAEELRDVKGMQSQHIQARGERHNYEVIHRDYWVSL
HHHHHHCCCEEECCCCCCCHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSS
CCCCCEEHHHHHHHHCHHHHHHHHHHHHHCCHHEEEEEEEEECCCCEEEEEEEEEEECCC
SLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCCCCCCCEEEECCHH
AAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIIN
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
ENDSISLEELTFGDNDMLSALVSGLVLADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHH
TEEIASLTGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCC
VGNAPQKEMKMNKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGVFQVGEV
CCCCCHHHHHHCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VEVMTKQGRVIGKGQCTYSAEELRDVKGMQSQHIQARGERHNYEVIHRDYWVSL
HHHHHHCCCEEECCCCCCCHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]