Click here to switch to the map view.

The map label for this gene is ykfB [H]

Identifier: 118478201

GI number: 118478201

Start: 2719067

End: 2720146

Strand: Reverse

Name: ykfB [H]

Synonym: BALH_2558

Alternate gene names: 118478201

Gene position: 2720146-2719067 (Counterclockwise)

Preceding gene: 118478202

Following gene: 118478200

Centisome position: 51.74

GC content: 37.69

Gene sequence:

>1080_bases
TTGCTAGATAAAGAAAGGGTGAACAAAATGAAAATTACCGATGTAAAAGTAAATCGTAGACGTGTAAATCTTCATACACC
GTTTAAAACAGCTCTTCGTACCGTAACCGAAATAGAAAGTATAGATGTATATATTCATACAGATGAAGGAATTGTCGGTA
AGGGAGCTGGAGCTGCAACGCCGGTTATTACGGGTGATTTCGCTAACGGGATTGAAGAAGCAATTTTAGGGCCGATGCGT
TCAAGTTTAATTGGTCAAGATATCATTCAATTTCAGCAGTTACTACAGCACATTCAAGTGAGTTGCATTGGAAATCCAAG
TGCGAAAGCAGCGGTAGATATCGCATTATATGATGTGTATTGTCAATATTGTAACGTTCCACTTTACGCATTATTAGGCG
GGAAGAAAGAAATTCATACAGATATTACGGTGAGTGTAGATGAGCCTTTCATTATGGCGAAAGAGGCAAAGCAACATGTA
GAAAAAGGATTTCAAACTTTAAAAATTAAAGTGGGGAAATCTGCACACTTAGACTTAGAACGTATTGAAGCGATTCGAAA
TAGCGTACCAAAAAATACGACGTTACGATTAGATGCAAATCAAGGGTGGAATCCAAAAGAGGCAGTTTCTATCATTAAAG
AGATGGAAAATCGCAATTTAAATATTGAATTTATTGAACAACCTGTTCATGCGAAAGATTGGGATGGATTAAAGTACGTC
AAAGATCATGTACAAACACCTATTATGGCAGATGAAAGCATATTTTCAGCGAGTGATGCATTAAAAATTGTGCAAGGAAG
ATATGCAGACTTAATCAATATTAAATTAATGAAATGTGGTGGCATTCGCGAAGCGTGGCGTATTGCCGATATCGCAGAAG
CAGCTGGTGTGAAATGTATGGTAGGAAGCATGATGGAATCTTCTCTTTCTGTTAGTGCTGTTGCACATTTAGCTGCTGCA
CATCCTAATATTCATTACTTCGATCTTGATGCGCCACTTTGGTTAATGGAAGAACCGGAAGGAATGACTTATTCTGGATC
AAAGGTAAACCTTCAATCGACAGTGAATAGTAAATCTTAG

Upstream 100 bases:

>100_bases
TTATGGCTGGATTTTTTTATTTCACATGTACCTTCTACATGGAAAGTATGCACAAGCGGAGATCTTTTGATCCAAAAAAG
TTAAGAATTAAGGCTCTATT

Downstream 100 bases:

>100_bases
GAGAAGAGACTAGTAAATTATCTTTTAAGGGGGATATTTATGAAAAAAGTAGGAACTGCATTTTTAACAACTTTATTTAT
ATTTTCATCGTTTACATCAG

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLDKERVNKMKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFANGIEEAILGPMR
SSLIGQDIIQFQQLLQHIQVSCIGNPSAKAAVDIALYDVYCQYCNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHV
EKGFQTLKIKVGKSAHLDLERIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYV
KDHVQTPIMADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAA
HPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS

Sequences:

>Translated_359_residues
MLDKERVNKMKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFANGIEEAILGPMR
SSLIGQDIIQFQQLLQHIQVSCIGNPSAKAAVDIALYDVYCQYCNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHV
EKGFQTLKIKVGKSAHLDLERIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYV
KDHVQTPIMADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAA
HPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
>Mature_359_residues
MLDKERVNKMKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFANGIEEAILGPMR
SSLIGQDIIQFQQLLQHIQVSCIGNPSAKAAVDIALYDVYCQYCNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHV
EKGFQTLKIKVGKSAHLDLERIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYV
KDHVQTPIMADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAA
HPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=331, Percent_Identity=32.6283987915408, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994953, Length=307, Percent_Identity=29.3159609120521, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39710; Mature: 39710

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKERVNKMKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAAT
CCCHHHCCCEEEEEEEEECEEEEECCCHHHHHHHHHHHEEEEEEEECCCCEEECCCCCCC
PVITGDFANGIEEAILGPMRSSLIGQDIIQFQQLLQHIQVSCIGNPSAKAAVDIALYDVY
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEHHHHHHH
CQYCNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKIKVGKSAHLDLE
HHHHCCCEEEEECCCEEECCCEEEEECCCEEEHHHHHHHHHCCCEEEEEEECCCCCCCHH
RIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYV
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHH
KDHVQTPIMADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCM
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHH
VGSMMESSLSVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEECCCCCCCC
>Mature Secondary Structure
MLDKERVNKMKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAAT
CCCHHHCCCEEEEEEEEECEEEEECCCHHHHHHHHHHHEEEEEEEECCCCEEECCCCCCC
PVITGDFANGIEEAILGPMRSSLIGQDIIQFQQLLQHIQVSCIGNPSAKAAVDIALYDVY
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEHHHHHHH
CQYCNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKIKVGKSAHLDLE
HHHHCCCEEEEECCCEEECCCEEEEECCCEEEHHHHHHHHHCCCEEEEEEECCCCCCCHH
RIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYV
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHH
KDHVQTPIMADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCM
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHH
VGSMMESSLSVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]