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The map label for this gene is acoC [H]

Identifier: 118478135

GI number: 118478135

Start: 2655631

End: 2656833

Strand: Reverse

Name: acoC [H]

Synonym: BALH_2492

Alternate gene names: 118478135

Gene position: 2656833-2655631 (Counterclockwise)

Preceding gene: 118478136

Following gene: 118478134

Centisome position: 50.54

GC content: 36.33

Gene sequence:

>1203_bases
ATGGCTGTAGAAGTTGTAATGCCGAAGTTAGGGATGGCGATGAAAGAGGGTATTATTACGAGCTGGAATATTAAAGCGGG
TGATAATGTGGCAAAAGGAGAATTAATTGCCAGTATTAACTCGGAAAAAATTGAAACGGAAATAGAAGCACCAGCTGATG
GAACTGTTCTTGATATAGCTGTAAGTGAAGATGAGGGGGTTCCACCTGGCACTGTAATTTGCTACATTGGTAAACCGAAT
GAAAAAGTAGAGGTACATGAAAGCACAAAGGTTGAGGAAGAACTGACAGCGAGTGAAGTACCACAAAATGTACAACATCC
AGAACCGTTAGGGAAAGAAGTTACGAATAAACAAAGAATCAAAATATCTCCTGTAGCGAAGAAGATTGCGAAAACTGAAA
ATCTTGATATTAAATCATTACTTGGTACCGGTCCAGGTGGAAGAATTACAAAGGTAGATGTATTAAAGGCGCTTGAAGAA
AGAGTAGCAGTTCCAGAAGTACTTGAACAAGAAGAAAGTAAAGTAATTCCTGTTACTGGTATGCGGAAAGCAATCGCAAA
CCGTATGCATGCAAGCTTACAAAATAGTGCACAATTAACATTAACGATGAAAGTAGATGTGACAGATTTAGTTGCTTTAC
ATAAAGAAATAGCAGAAGTTGTACAAAAACGGTACGATAACAAATTAACCATTACGGATTTCGTTTCTCGAGCTGTTGTA
TTAGCGCTTGGGGATCATAAAGAAATGAACAGCGCTTATATAGATGATGCAATTCATCAATTTGAACATGTTCATTTAGG
TATGGCAGTCGCATTAGAAAAAGGATTAGTCGTTCCAGCTATTCGCTTCGCAAATAATCTCTCATTAGTAGAGTTATCTA
AAGAGATTAAGAATGTGGCACAAAAGGCACGAGCAGGCAATTTAAATAGTGATGATATGCAAGGAACAACATTTACAATT
AGTAATTTAGGAAGCTTCGGTATTGAATATTTTACACCAGTATTAAATACACCAGAAACTGGTATTTTAGGTGTAGGTGC
AATTGAATATGTTCCTGTATATAAAGGAAAAAAATTAAAAAAAGGAAGTATGTTACCTTTAAGTTTAACATTCGATCATC
GTGTACTAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGCTATTTAGAAGAACCTGTAACAATTCTTTTA
TAA

Upstream 100 bases:

>100_bases
TTTACCAACGCCGGAGAAAGTAATTGAAACTGTATCAGAAATGATCGGAGACCAATCTTTATTACATGTGTAATGAAACG
ATGAGAAGGGAGAGAGAAAT

Downstream 100 bases:

>100_bases
AGAGAAGGTGAGAGAATGAGTAAATTAGTCGTTATTGGAGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCGGCTCA
AAATGGAAAAAATGTCACTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPN
EKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEE
RVAVPEVLEQEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVV
LALGDHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNVAQKARAGNLNSDDMQGTTFTI
SNLGSFGIEYFTPVLNTPETGILGVGAIEYVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Sequences:

>Translated_400_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPN
EKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEE
RVAVPEVLEQEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVV
LALGDHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNVAQKARAGNLNSDDMQGTTFTI
SNLGSFGIEYFTPVLNTPETGILGVGAIEYVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
>Mature_399_residues
AVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPNE
KVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEER
VAVPEVLEQEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVVL
ALGDHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNVAQKARAGNLNSDDMQGTTFTIS
NLGSFGIEYFTPVLNTPETGILGVGAIEYVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=30.6264501160093, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=26.5116279069767, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=25.9179265658747, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=25.9179265658747, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.1772151898734, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=31.7073170731707, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=28.9099526066351, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=30.6264501160093, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=411, Percent_Identity=27.4939172749392, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25.2380952380952, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=221, Percent_Identity=28.9592760180996, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=30.9226932668329, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=26.6816143497758, Blast_Score=163, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=31.2925170068027, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=31.2925170068027, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=25.6944444444444, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=236, Percent_Identity=33.0508474576271, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43494; Mature: 43363

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIA
CCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHHEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRI
ECCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEE
KISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEERVAVPEVLEQEESKVIPVTG
EECHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCEECCCH
MRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVV
HHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHEEE
LALGDHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNVA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCHHHHHHHHHHHHH
QKARAGNLNSDDMQGTTFTISNLGSFGIEYFTPVLNTPETGILGVGAIEYVPVYKGKKLK
HHHHCCCCCCCCCCCCEEEECCCCHHCHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCC
KGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
AVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIA
CEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHHEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRI
ECCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEE
KISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEERVAVPEVLEQEESKVIPVTG
EECHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCEECCCH
MRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVV
HHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHEEE
LALGDHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNVA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCHHHHHHHHHHHHH
QKARAGNLNSDDMQGTTFTISNLGSFGIEYFTPVLNTPETGILGVGAIEYVPVYKGKKLK
HHHHCCCCCCCCCCCCEEEECCCCHHCHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCC
KGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]