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The map label for this gene is pbpE [H]

Identifier: 118478037

GI number: 118478037

Start: 2563778

End: 2564812

Strand: Direct

Name: pbpE [H]

Synonym: BALH_2389

Alternate gene names: 118478037

Gene position: 2563778-2564812 (Clockwise)

Preceding gene: 118478036

Following gene: 118478038

Centisome position: 48.77

GC content: 32.56

Gene sequence:

>1035_bases
GTGGTTTTTGTAATGAAAACAAAAGATCAAATAGAGAGCATTGTAAAAGAAATGCATCGGCATATAGATTTCTCTGGGGT
AATTTTAGTGAAAGAAAAAAAGGGCATAGTTTATGAAGAGGCGTTTGGTTATGCAAATCGAAATGAGTGTATAAACAATA
CGCTACAAACTAGGTTTGGAATTGCCTCCGGATGTAAAATATTCACAGCTACCGGCATCTGTAAACTTGTGGAAAATGGG
GTAATTACTTTTCAAACAAAGTTAAAAGATTGTTTAAGTGTTAACTTTCCAAACTTTAATGAAGATATTACAATACATCA
CCTTTTGACGCATAGTTCGGGTATTCCAGATTATTTTGATGAAAGTATTATGGATAACTTCGAAGATCTTTGGAAACAAA
CACCTATGTATTTATTAAAAAGTTTAAAAGACTTTTTACCATTATTTCAAAACAGAGATATGATGTATACACCTGGAAGT
AAGTTTCACTACAACAATGCAAGTTTTATCATACTGGGATTAATAATTGAAGAACAGACAGGACTTACATTTACGGAGTA
TATAGAAACAAACATATTTAATCCAATTGGTATGAATCATTCGGGTTATTTTTCTTTAGATAGATTACCAAGGCAGACAG
CTCTCGGATATATAAAAGATGAAATTAGCCAGACATGGAGAACAAATGCTTACTCTATTCCGATAAAAGGTGGTTCTGAT
GGTGGAGCTTTTATTACTGCGTCAGACATGCTGAAGTTTTGGGAAGCGTTATTTAACTATGAAATTATAAGCCAAGAATA
TACAAAAATACTTTTAACTCCTCACATTCAAGTAAATAATAATCAATCTTATGGCTATGGAATATGGATTGAAACAAGAG
AAAACAAGATATTCAAATATCATGTAATGGGATATGACCCAGGCGTCAGTTTTCGCTCGGCCGTATATCCAGACTTAGGA
ATTACATTAGTTATTCCATCAAATAAAGGAGCAGGACCCGAAAAATTAATGATAGAAATAGAAGGGGATTTTTAA

Upstream 100 bases:

>100_bases
AAAATAGTTCCCTTGGTTTTGATTTACGCTATTCAATAGGAGGGTTCACCCTCCTATATATGGAAATATAAGAAGGGTGA
ACAATTATTGATAAACAAGG

Downstream 100 bases:

>100_bases
TTATATGAACATAGAAATTAGAGCGTATAGAAAAGAGGATGAAACTGGTTGGGTACGTTGCCGTGTGTTATCTTTTTTAG
ATACAGTTTATTACGATAAT

Product: penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MVFVMKTKDQIESIVKEMHRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASGCKIFTATGICKLVENG
VITFQTKLKDCLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYTPGS
KFHYNNASFIILGLIIEEQTGLTFTEYIETNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSD
GGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVYPDLG
ITLVIPSNKGAGPEKLMIEIEGDF

Sequences:

>Translated_344_residues
MVFVMKTKDQIESIVKEMHRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASGCKIFTATGICKLVENG
VITFQTKLKDCLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYTPGS
KFHYNNASFIILGLIIEEQTGLTFTEYIETNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSD
GGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVYPDLG
ITLVIPSNKGAGPEKLMIEIEGDF
>Mature_344_residues
MVFVMKTKDQIESIVKEMHRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASGCKIFTATGICKLVENG
VITFQTKLKDCLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYTPGS
KFHYNNASFIILGLIIEEQTGLTFTEYIETNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSD
GGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKYHVMGYDPGVSFRSAVYPDLG
ITLVIPSNKGAGPEKLMIEIEGDF

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533026, Length=272, Percent_Identity=26.1029411764706, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17550934, Length=194, Percent_Identity=26.8041237113402, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 39354; Mature: 39354

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFVMKTKDQIESIVKEMHRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFG
CEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHCCCCCCHHHHHHHHHHHHC
IASGCKIFTATGICKLVENGVITFQTKLKDCLSVNFPNFNEDITIHHLLTHSSGIPDYFD
CCCCCEEEEHHHHHHHHHCCEEEEEEHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHH
ESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYTPGSKFHYNNASFIILGLIIEEQT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEEECCC
GLTFTEYIETNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSD
CCCHHHHHHHHCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKY
CCEEEEHHHHHHHHHHHHCHHHCCCCCEEEEEECEEEECCCCCCEEEEEEEECCCEEEEE
HVMGYDPGVSFRSAVYPDLGITLVIPSNKGAGPEKLMIEIEGDF
EEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVFVMKTKDQIESIVKEMHRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFG
CEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHCCCCCCHHHHHHHHHHHHC
IASGCKIFTATGICKLVENGVITFQTKLKDCLSVNFPNFNEDITIHHLLTHSSGIPDYFD
CCCCCEEEEHHHHHHHHHCCEEEEEEHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHH
ESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYTPGSKFHYNNASFIILGLIIEEQT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEEECCC
GLTFTEYIETNIFNPIGMNHSGYFSLDRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSD
CCCHHHHHHHHCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GGAFITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKIFKY
CCEEEEHHHHHHHHHHHHCHHHCCCCCEEEEEECEEEECCCCCCEEEEEEEECCCEEEEE
HVMGYDPGVSFRSAVYPDLGITLVIPSNKGAGPEKLMIEIEGDF
EEEEECCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]