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The map label for this gene is yrkH [H]

Identifier: 118477938

GI number: 118477938

Start: 2453502

End: 2454953

Strand: Direct

Name: yrkH [H]

Synonym: BALH_2284

Alternate gene names: 118477938

Gene position: 2453502-2454953 (Clockwise)

Preceding gene: 118477937

Following gene: 118477939

Centisome position: 46.67

GC content: 35.4

Gene sequence:

>1452_bases
TTGGAGGGTATAGCTATGCTTTTAAAGTATTTTTATGATGAAAAATTAGCACATGCTTCCTATTTAGTCGGTTGTCAGAA
GGAAGGTGTAGCAATTGTAATTGATCCAGGTCGCTATATAGAACAATATATAGAATTCGCTAAGAAAGAGGGGATGGAAG
TAACAGCTGCAGCTGAAACTCACATTCATGCAGATTTTCTTTCTGGGTCTAGAGAACTTTCTATTCTTTATCATGCCAAT
TTATATATATCTGATGAAGGTGATTGTGATTGGAAATATCAATATCTTAACGAAGGTCAATACAATTTGGTTAGAGAGGG
GACAGAGTTTAAAGTGGGTCATATAAAATTTAGTGTAATTCACACACCAGGGCATACACCTGAAAGTATTTCTTTTTTAG
TAACTGATACAAGTCAAAATAACTATACTAATGATAAGCCAATAGGAATATTCACAGGTGATTTTATATTTGTCGGAGAT
ATAGGAAGACCCGATTTATTAGAAACTGCTGTAGGTATGAAAGATACCGCAAAAATAGGAGCAAAACAATTATTTGATTC
GATACAAAAAATAAAAACACTCCCTGATTATTTGCAAATATGGCCATCACATGGTGCAGGAAGCGCATGTGGAAAAGCAT
TAGGAGCAATTCCAACTTCTACCTTAGGTTATGAAAAGATGTTCAATTGGGCATTTCAGTGTAATGAAGAAAGTGATTTT
ATATCGACTTTATTGACGGGGCAGCCAGAGCCTCCAAGGTATTTTTCTTTAATGAAGAATTTAAATAAGTATGGTCCTCC
CATTCGTAAGAAAAGAGAGATTTTTGCTATTAAAACAGTAGAGGAACTTCAAGAAGTTATGAGGAGCGTTCAGCAAATAG
TTGATATAAGGGATGTAGAGAGTTTTGCTTCTGGTCACATCGAGAAATCAATTAACATACCGTATAACAATTCCTTCACA
ACCTGGTGTGGATGGTTACTAGATTATAAAACAGAAACTTTAATCATTCTAGATGAGGAAAAGGTTAGAGTGGAGGAGGT
TATTAGAGACTTTGAATCTATTGGGTTAGATAATAGTATTGCCTTTGCACCCTTACAAGTAATACAAAGACTCGATAGAT
TTGAAAGTTACAAAGAAAAAACATCGATTGAATTATATCCGCATATAAAGGATGGTAGGGTTAAGGTAATTGATGTACGT
AGTAAAAAAGAGTGGGACGAAGGACATCTTCATGATGCAATACATATCCCTTTAGGAAATCTATTGAAGCAGCTAGATTG
TATACCAAAAGATTGCCCAATCGTTTTACAATGTCGAACCGGATTGCGTTCCGCTATAGCGGCTAGTATTTTACAAAGAG
CTGGTATAAAGGGAGTGGTGAATCTAAAGGGAGGGTTCCTTGCATGGAAAAAAGAAGAGCTTCCTTATATAACTTGTAGT
TTCAATGTGTAG

Upstream 100 bases:

>100_bases
TATGTTGCCCTATATGTATTGGAACTTTATGTTGAAAGGGATCCTATAGGAGAATATAGAAAGCGGTTATAAATGTTGAA
AAGTAATTTGAAATAACTAT

Downstream 100 bases:

>100_bases
GGAAATGATTTTTATTAATTTATCCGATAAGGAAAATTGCGGATTACATAGGAATAGGAGTATATCTATATATAGGAGCA
CCACATTAAAATAGGAGAAA

Product: hydroxyacylglutathione hydrolase

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MEGIAMLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVTAAAETHIHADFLSGSRELSILYHAN
LYISDEGDCDWKYQYLNEGQYNLVREGTEFKVGHIKFSVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGD
IGRPDLLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDF
ISTLLTGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDVESFASGHIEKSINIPYNNSFT
TWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNSIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVR
SKKEWDEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKGVVNLKGGFLAWKKEELPYITCS
FNV

Sequences:

>Translated_483_residues
MEGIAMLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVTAAAETHIHADFLSGSRELSILYHAN
LYISDEGDCDWKYQYLNEGQYNLVREGTEFKVGHIKFSVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGD
IGRPDLLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDF
ISTLLTGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDVESFASGHIEKSINIPYNNSFT
TWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNSIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVR
SKKEWDEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKGVVNLKGGFLAWKKEELPYITCS
FNV
>Mature_483_residues
MEGIAMLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVTAAAETHIHADFLSGSRELSILYHAN
LYISDEGDCDWKYQYLNEGQYNLVREGTEFKVGHIKFSVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGD
IGRPDLLETAVGMKDTAKIGAKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDF
ISTLLTGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDVESFASGHIEKSINIPYNNSFT
TWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNSIAFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVR
SKKEWDEGHLHDAIHIPLGNLLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKGVVNLKGGFLAWKKEELPYITCS
FNV

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=205, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787158, Length=227, Percent_Identity=25.5506607929515, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 54670; Mature: 54670

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGIAMLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVTAAAET
CCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHH
HIHADFLSGSRELSILYHANLYISDEGDCDWKYQYLNEGQYNLVREGTEFKVGHIKFSVI
HEEHHHHCCCCEEEEEEEEEEEEECCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEEE
HTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGMKDTAKIG
ECCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHH
AKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDF
HHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHH
ISTLLTGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDVE
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
SFASGHIEKSINIPYNNSFTTWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNSI
HHHCCCCEEEEECCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
AFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEWDEGHLHDAIHIPLGN
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCHHH
LLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKGVVNLKGGFLAWKKEELPYITCS
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEE
FNV
ECC
>Mature Secondary Structure
MEGIAMLLKYFYDEKLAHASYLVGCQKEGVAIVIDPGRYIEQYIEFAKKEGMEVTAAAET
CCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHH
HIHADFLSGSRELSILYHANLYISDEGDCDWKYQYLNEGQYNLVREGTEFKVGHIKFSVI
HEEHHHHCCCCEEEEEEEEEEEEECCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEEE
HTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGMKDTAKIG
ECCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHH
AKQLFDSIQKIKTLPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFQCNEESDF
HHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHH
ISTLLTGQPEPPRYFSLMKNLNKYGPPIRKKREIFAIKTVEELQEVMRSVQQIVDIRDVE
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
SFASGHIEKSINIPYNNSFTTWCGWLLDYKTETLIILDEEKVRVEEVIRDFESIGLDNSI
HHHCCCCEEEEECCCCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
AFAPLQVIQRLDRFESYKEKTSIELYPHIKDGRVKVIDVRSKKEWDEGHLHDAIHIPLGN
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEECCHHH
LLKQLDCIPKDCPIVLQCRTGLRSAIAASILQRAGIKGVVNLKGGFLAWKKEELPYITCS
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEE
FNV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]