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The map label for this gene is entE [H]

Identifier: 118477771

GI number: 118477771

Start: 2259921

End: 2261537

Strand: Direct

Name: entE [H]

Synonym: BALH_2109

Alternate gene names: 118477771

Gene position: 2259921-2261537 (Clockwise)

Preceding gene: 118477770

Following gene: 118477772

Centisome position: 42.99

GC content: 38.59

Gene sequence:

>1617_bases
ATGTTAACAGGTTATACAAAATGGCCAAAAGAATTTGCCGATCGCTATCGAGAGGAAGGGTGTTGGCTTGGGGAAACATT
TGGCGGAGTGTTAAGAGGGCGTGCTGAAAAATATGGAGATCAAATTGCGGTTGTAAGTGGTAATACGTATATAACATATA
GTGAGCTTGATAAAAAAGTAGATCGCTTAGCGTCAGGTTTACTGAATTTAGGAATAAAGCCAGAAGATCGAGTTGTAATC
CAACTACCTAACGTTATCGAGTTTTTTGAAATGTGTTTTGCGCTATTTCGAATTGGAGCACTTCCTGTTTTTGCATTACC
TTCACATCGCAGTAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCGTACGTTATTTCAGATAAGGCTCTTG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTACCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAGTTTGTGAACATAAGTGATTTATATATAGATCCCGTTCTATTGCCAGAAGTTCAGCCAAGTGATGTTGCCTTTCT
CCAATTATCAGGAGGGACAACAGGACTTTCTAAATTAATTCCTAGAACGCATGATGACTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAGAGAGCGTCTATATGGCAGTTCTTCCAGTAGCACATAATTACCCAATGAGTTCT
CCTGGAACATTTGGAACCTTTTATGCGGGTGGAAAAGTTGTGCTGGCAACTGGGGGTAGTCCGGATGAGACATTTGCACT
TATCGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCGCCATTAGCAATGATATGGCTTGACGCTGCATCTTTACGTA
ATAACGATTTATCAAGCTTAGAAGTGATTCAAGTAGGTGGTGCGAAATTTAGTGCTGAAGTTGCAAAACGTATACGTCCT
ACATTTGGATGTACATTACAGCAAGTGTTCGGTATGGCAGAAGGATTAGTGAACTATACAAGATTAAACGATCCGGAAGA
AATTATAATTCATACACAAGGTAGACCAATGTCTGCACTTGATGAAGTACGAGTTGTTGACGAATATGATAACGATGTAA
AACCTGGTGAAGTAGGCAGTTTATTAACACGGGGTCCATATACAATTCGTGGATACTATAAAGCAGAAGAGCATAATGCA
CGATCATTTACAAAGGATGGATTTTATCGCACAGGAGATCTTGTAAAAGTAAACGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAAAAAGTCGCTGCGGAAGAAGTTGAAAATCATCTATTAGCACATGATG
CAGTACATGATGTAGCGATTGTATCGATGCCTGACGATTATTTAGGAGAACGTACTTGTGCCTTTGTAATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAAGGTTCTTAAGAGAACGCGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGTGTGGGAAAAGTGAGCAAAAAAGAATTACGTAAAGTCATTGCGGAAAAACTTG
TTACGGTAAAACGATAA

Upstream 100 bases:

>100_bases
AGAAGATGAATTAGCAGAAACATCAGCTAAGTTTCAAACAATGTTGAAAGCTTTAGGGTTAAATGATAGTTCACTTAATG
AAAAGTAGGGGGCAGAAAGA

Downstream 100 bases:

>100_bases
GAAAATATAAAACTTGGAAGGAAGTGATTAGATGGCTATCCCATCTATTTCAGTATACAAAATGCCAATTGAATCAGAAC
TACCAAAAAATAAAGTGAAC

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: diphosphate; (2,3-dihydroxybenzoyl)adenylate

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRGRAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNISDLYIDPVLLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLNDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR

Sequences:

>Translated_538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRGRAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNISDLYIDPVLLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLNDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR
>Mature_538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRGRAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNISDLYIDPVLLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLNDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=24.8188405797101, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=26.1194029850746, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=26.1194029850746, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI28416953, Length=522, Percent_Identity=25.8620689655172, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI38505220, Length=499, Percent_Identity=23.8476953907816, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI42544132, Length=475, Percent_Identity=23.7894736842105, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157311624, Length=492, Percent_Identity=24.1869918699187, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI157311622, Length=492, Percent_Identity=24.1869918699187, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI122937307, Length=495, Percent_Identity=23.4343434343434, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=24.2125984251968, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI58082049, Length=499, Percent_Identity=23.6472945891784, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8923896, Length=567, Percent_Identity=24.8677248677249, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI253970489, Length=149, Percent_Identity=32.8859060402685, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1786810, Length=541, Percent_Identity=48.6136783733826, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=31.390977443609, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788107, Length=532, Percent_Identity=28.0075187969925, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI221142682, Length=512, Percent_Identity=24.609375, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=23.2902033271719, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789201, Length=356, Percent_Identity=25.2808988764045, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786529, Length=522, Percent_Identity=23.3716475095785, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786801, Length=451, Percent_Identity=21.7294900221729, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=27.7173913043478, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=27.7173913043478, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=27.7173913043478, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=28.0898876404494, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17531443, Length=517, Percent_Identity=25.7253384912959, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=535, Percent_Identity=27.2897196261682, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=26.654740608229, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=24.9056603773585, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=528, Percent_Identity=24.4318181818182, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=491, Percent_Identity=21.7922606924644, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=482, Percent_Identity=21.5767634854772, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=535, Percent_Identity=23.7383177570093, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=561, Percent_Identity=24.5989304812834, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=561, Percent_Identity=24.5989304812834, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=24.5179063360882, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI193209603, Length=476, Percent_Identity=21.8487394957983, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556356, Length=355, Percent_Identity=23.3802816901408, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=28.051948051948, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323182, Length=550, Percent_Identity=25.2727272727273, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=22.1590909090909, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24581924, Length=547, Percent_Identity=25.4113345521024, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=23.21083172147, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=23.3521657250471, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24667955, Length=554, Percent_Identity=25.812274368231, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=25.7943925233645, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922652, Length=533, Percent_Identity=22.8893058161351, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24656500, Length=533, Percent_Identity=23.2645403377111, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355225, Length=568, Percent_Identity=22.7112676056338, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667959, Length=382, Percent_Identity=25.3926701570681, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648260, Length=534, Percent_Identity=21.9101123595506, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356441, Length=496, Percent_Identity=23.5887096774194, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=518, Percent_Identity=22.007722007722, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=475, Percent_Identity=22.9473684210526, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356947, Length=504, Percent_Identity=20.2380952380952, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648255, Length=518, Percent_Identity=22.007722007722, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21358303, Length=487, Percent_Identity=20.7392197125257, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653035, Length=305, Percent_Identity=21.3114754098361, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921316, Length=239, Percent_Identity=26.7782426778243, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58

Molecular weight: Translated: 59752; Mature: 59752

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRGRAEKYGDQIAVVSGNTYITYSELDKKV
CCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCEEEEEECCEEEEHHHHHHHH
DRLASGLLNLGIKPEDRVVIQLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEF
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
GEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEEEEFVNISDLYIDPVLLPEVQ
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHEEECCEECCCCC
PSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHHCCCHHHEEEEEEEHCCCCCCCC
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSL
CCCCEEEECCCEEEEEECCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
EVIQVGGAKFSAEVAKRIRPTFGCTLQQVFGMAEGLVNYTRLNDPEEIIIHTQGRPMSAL
EEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
DEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNARSFTKDGFYRTGDLVKVNEQ
HHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCEEEECCC
GYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCEEEEEEECC
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR
CCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRGRAEKYGDQIAVVSGNTYITYSELDKKV
CCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCEEEEEECCEEEEHHHHHHHH
DRLASGLLNLGIKPEDRVVIQLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEF
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
GEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEEEEFVNISDLYIDPVLLPEVQ
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHEEECCEECCCCC
PSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHHCCCHHHEEEEEEEHCCCCCCCC
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSL
CCCCEEEECCCEEEEEECCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
EVIQVGGAKFSAEVAKRIRPTFGCTLQQVFGMAEGLVNYTRLNDPEEIIIHTQGRPMSAL
EEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
DEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNARSFTKDGFYRTGDLVKVNEQ
HHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCEEEECCC
GYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCEEEEEEECC
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR
CCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 2,3-dihydroxybenzoate

Specific reaction: ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]