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The map label for this gene is narH [H]

Identifier: 118477563

GI number: 118477563

Start: 2040508

End: 2041983

Strand: Direct

Name: narH [H]

Synonym: BALH_1889

Alternate gene names: 118477563

Gene position: 2040508-2041983 (Clockwise)

Preceding gene: 118477562

Following gene: 118477564

Centisome position: 38.81

GC content: 38.82

Gene sequence:

>1476_bases
TTGAAGATTAAAGCACAAGTCGGAATGGTAATGAACCTAGATAAATGCATCGGCTGCCATACTTGTAGCGTAACATGCAA
AAACACCTGGACAAATCGTCCAGGTGCTGAATATATGTATTTCAATAACGTAGAAACAAAACCCGGTATTGGTTATCCGA
AGCAATGGGAAGATCAGGAGAAATATAAAGGCGGTTGGGAATTAAAAAATGGTGAAATTCAGCTGAAATCCGGTTCGAAA
ATGAAGCGCCTTATGAATATTTTCCACAATCCAGATCAACCAACAATTGATGATTACTTTGAACCGTGGAACTATGATTA
TGAAACATTAACAAATAGTCCGCAGCGTAAGCATCAGCCAGTTGCTCGTCCAAAATCAGCAATTACAGGCGAATTTATCG
ACAAAATTGAATGGGGTCCAAACTGGGAAGATGATTTAGCTGGTGGACATATAACAGGATTACAAGATCCGAACGTAAAG
AAAATGGAAGAAGAAATTAAAACCGATTTTGAAAATGTCTTTATGATGTATTTACCACGCATATGCGAACATTGTATGAA
TCCATCTTGCGTATCGTCTTGTCCATCTGGTGCGATGTATAAACGGGAAGAAGATGGGATTGTTCTTGTAGATCAAAATG
CATGTCGTGCATGGAGATTCTGCGTATCGTCTTGCCCATATAAAAAAGTGTACTTTAATTGGCAAACGAATAAAGCGGAA
AAATGTACAATGTGTTTCCCACGTATTGAAGCTGGTATGCCAACAATTTGTTCGGAAACATGTGTAGGACGTATCAGATA
TATTGGGGTAATGCTATATGACGCTGACAAAGTAAAAGAAGCGGCTTCTGTTGAGAATGAAAAAGATTTATATGAATCTC
AGCTTACTGTTTTCCTAGATCCAAATGATCCCGAAGTAGCAGCTGAAGCGAAAAAACAAGGCATTCCTGAAGAGTGGATT
AAAGCGGCACAACAGTCACCAATCTATAAAATGATTATCGATTGGAAAATTGCTCTACCTCTTCATCCGGAGTATCGTAC
AATGCCAATGGTTTGGTATATCCCACCGCTTAGCCCAATTATGAATATGGTGGAGGGGAAAGGTAGTAACTGGCAAGCAG
AAGAGATTTTCCCTGCTATCGATAATATGCGTATTCCAATTCAATATTTAGCGAATCTACTCACTGCAGGAGATGAATCA
CACATTCGACTTACATTGAAAAAAATGGCTGTTATGCGAACGCATATGAGAGCACTGCAAATTAATAAAGAACCAAATGA
AGCTGTATTAAAAGAACTTGGTTTAACGAAACAGGATGTAGAAGATATGTATCGTCTTCTTGCCATTGCAAAATATAAAG
ATCGCTTCGTCATCCCGACATCTCATCGTGAACAAGTGGCAGATTTATACAGCGAGCAAGGAAGCTGTGGTTTATCATTT
GCAGGTGGTCCAGGTTCTTGTATGACAATTTCTTAA

Upstream 100 bases:

>100_bases
GGATATGGTCAATTAAGTTATGGATTTAACTATTATGGTCCAACTGGGAACCAGCGCGATTTAAACGTTGTAATTCGCAA
ACTGAAGGAGGTAGATTGGC

Downstream 100 bases:

>100_bases
ATAATGGGGGCGGAAAAATATGAAACAAAGTCTACAAACTGCTTTTTCTTGTTCTTCATTCCTTCTCTCTTACCCGGAAC
TTGGGTGGAGAGAAGCTTTA

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWI
KAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
AGGPGSCMTIS

Sequences:

>Translated_491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWI
KAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
AGGPGSCMTIS
>Mature_491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWI
KAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
AGGPGSCMTIS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=60.3305785123967, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=487, Percent_Identity=59.5482546201232, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 56201; Mature: 56201

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQE
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQP
HHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCHHHCCC
VARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCC
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLD
CHHEECHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHCCCHHHHHHCCEEEEEC
PNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPI
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHH
MNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQ
HHHHCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHH
INKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCCEEE
AGGPGSCMTIS
CCCCCCEEEEC
>Mature Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQE
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQP
HHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCHHHCCC
VARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCC
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLD
CHHEECHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHCCCHHHHHHCCEEEEEC
PNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPI
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHH
MNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQ
HHHHCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHH
INKEPNEAVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCCEEE
AGGPGSCMTIS
CCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]