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The map label for this gene is gatA [H]

Identifier: 118477506

GI number: 118477506

Start: 1983051

End: 1984562

Strand: Direct

Name: gatA [H]

Synonym: BALH_1831

Alternate gene names: 118477506

Gene position: 1983051-1984562 (Clockwise)

Preceding gene: 118477505

Following gene: 118477508

Centisome position: 37.72

GC content: 36.38

Gene sequence:

>1512_bases
ATGCTATCAATAAAGGGTTATAGAAGGAGAATGAAAATGGAGATGCAGTTCAATACACTATTACAAAAAGAATTAACAAT
TCATGATATACAAACAGCGATGGAAGATGAAAAGTTAACTTCAAAAGAATTAGTTATGTATTATCTTCATAGAATTGCAA
AGTATGATCAAGACGGACCGAAAATTAATTCTATTTTAGAAATAAATCCAGACGCTATTTTTATTGCAGAAACGTTAGAT
CATGAAAGAAAGATTAAAGGTGTAAGAGGTCCATTACATGGCATACCGGTTTTACTTAAGGACAATATTGAAACGAACGA
CTCTATGCATACAAGCGCAGGTACGATTGCGCTAGAACAACATATAAGTAGTGAAGATGCATTTCTCGTTACGAAGCTGA
GAGAAGCAGGAGCAGTGATACTCGGGAAAACAAATATGACAGAATTAGCAAATGCAATGTCTTTTGAAATGTGGGCTGGA
TATAGTGCAAGAGGTGGGCAAACAATCAATCCTTACGGTACGGGTGACGATGATATGTTTGTTGGTGGCTCAAGTACAGG
ATCTGCGATAGCAGTTGCTGCTAACTTTACAGTAGTATCAGTTGGAACAGAAACGGATGGTTCTATATTGAGTCCAGCTG
TTCAAAACTCTGTAGTCGGTATAAAACCGACTGTTGGTTTAATTAGTCGTAGAGGTATTATTCCGTTCACATATTCGCAA
GATACAGCCGGTCCATTTGCTAGAACCGTAACAGATGCAGCAATCTTACTAGGGAGTTTAACTGGGGTAGATGAGAAAGA
TGTAGCTACTCATAAAAGTGAAGGGATAGGAGAACCTGATTATACGAAATACCTTGATGTTAATGGGTTAAATGGAACAA
AGATTGGTGTATATAACAACGCACCAAAAGAATATTATGAAACTGGTGAGTATGATGAAAAATTGTTTAAGGAAACGATT
GAAGTATTGCATAGTAAGGGTGCGACAGTCGTAGAGGATATTCATATTCCATCTTTTCATAGAGAATGGAGCTGGGGAGT
ATCGCTCTATGAACTAAAGCATAGTTTAGATAACTATCTTTCGAAATTACCTTCTACTATTCCAGTACATTCTATTTCAG
AGTTAATGGAGTTTAATGAAAATATAGCAGAAAGGGCTTTGAAATATGGACAAACTAAGTTAGAAAGAAGAAAAGATTTT
CCTAATACTTTAAGGAATCCAGAGTATTTGAATGCAAGATTAGAAGATATATATTTTTCTCAAGAACAAGGCATTGATTT
TGCACTGGAAAAATATAATCTTGATGCGATTCTGTTCCCTTCTTATATAGGCTCCACTATATGTGCAAAAGCAGGTTATC
CGTCTATAGCAATACCTGCGGGATATATGGAAAGCGGGAGACCTTTTGGAATTACTCTTGCCAGTACTGCGTTTAGTGAG
GGGACATTAATTAAACTAGCATACGCTTTTGAACAAGCGACAAAGCATAGAAAAATTCCGGGATTGTCATAA

Upstream 100 bases:

>100_bases
TTCAGGAAATAAAAGGTTTTTATATTGGTAAGTTGAATTCTTATAGAGGGATTGTAGTTGTATATGTGAGGAGCGGAGAA
AATGAAAAAGTAATAGAGAC

Downstream 100 bases:

>100_bases
ATAGAATATATAAAAAAGGACAGGAGTTCCTGTCCTTTTTGCTTTATTGATTACATTGAAATATTTTTTTGATTTGTTTT
ATGATATTTTAATAGACTAA

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLSIKGYRRRMKMEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAETLD
HERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAG
YSARGGQTINPYGTGDDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQ
DTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYDEKLFKETI
EVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDF
PNTLRNPEYLNARLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMESGRPFGITLASTAFSE
GTLIKLAYAFEQATKHRKIPGLS

Sequences:

>Translated_503_residues
MLSIKGYRRRMKMEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAETLD
HERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAG
YSARGGQTINPYGTGDDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQ
DTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYDEKLFKETI
EVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDF
PNTLRNPEYLNARLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMESGRPFGITLASTAFSE
GTLIKLAYAFEQATKHRKIPGLS
>Mature_503_residues
MLSIKGYRRRMKMEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGPKINSILEINPDAIFIAETLD
HERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQHISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAG
YSARGGQTINPYGTGDDDMFVGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQ
DTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNNAPKEYYETGEYDEKLFKETI
EVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYLSKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDF
PNTLRNPEYLNARLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMESGRPFGITLASTAFSE
GTLIKLAYAFEQATKHRKIPGLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=536, Percent_Identity=26.4925373134328, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI195972892, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=486, Percent_Identity=25.1028806584362, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=24.3564356435644, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=263, Percent_Identity=28.1368821292776, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=209, Percent_Identity=25.8373205741627, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=480, Percent_Identity=26.25, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=209, Percent_Identity=37.3205741626794, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648113, Length=472, Percent_Identity=28.8135593220339, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24648435, Length=256, Percent_Identity=28.90625, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648437, Length=256, Percent_Identity=28.90625, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550774, Length=256, Percent_Identity=28.90625, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356731, Length=248, Percent_Identity=29.8387096774194, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24644968, Length=529, Percent_Identity=23.062381852552, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=23.2931726907631, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=23.2931726907631, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=23.2931726907631, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=23.2931726907631, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 55585; Mature: 55585

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIKGYRRRMKMEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KINSILEINPDAIFIAETLDHERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQ
CCCCEEEECCCEEEEEHHHCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHH
HISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGDDDMF
HCCCCCCEEHEEHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEE
VGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQ
ECCCCCCCEEEEEEEEEEEEECCCCCCCHHCHHHHCCCEECCCCHHHHHCCCCCEEEECC
DTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNN
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECC
APKEYYETGEYDEKLFKETIEVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYL
CCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCHHHHHHHHHHHHHH
SKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNARLEDIYFS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHEEC
QEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMESGRPFGITLASTAFSE
HHCCCEEEEEHCCCCEEEECHHHHHHHHHCCCCCCEEECCCHHCCCCCEEEEEECCCCCC
GTLIKLAYAFEQATKHRKIPGLS
CCEEEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSIKGYRRRMKMEMQFNTLLQKELTIHDIQTAMEDEKLTSKELVMYYLHRIAKYDQDGP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KINSILEINPDAIFIAETLDHERKIKGVRGPLHGIPVLLKDNIETNDSMHTSAGTIALEQ
CCCCEEEECCCEEEEEHHHCHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHH
HISSEDAFLVTKLREAGAVILGKTNMTELANAMSFEMWAGYSARGGQTINPYGTGDDDMF
HCCCCCCEEHEEHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEE
VGGSSTGSAIAVAANFTVVSVGTETDGSILSPAVQNSVVGIKPTVGLISRRGIIPFTYSQ
ECCCCCCCEEEEEEEEEEEEECCCCCCCHHCHHHHCCCEECCCCHHHHHCCCCCEEEECC
DTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGIGEPDYTKYLDVNGLNGTKIGVYNN
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECC
APKEYYETGEYDEKLFKETIEVLHSKGATVVEDIHIPSFHREWSWGVSLYELKHSLDNYL
CCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHCCHHHHHHHHHHHHHH
SKLPSTIPVHSISELMEFNENIAERALKYGQTKLERRKDFPNTLRNPEYLNARLEDIYFS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHEEC
QEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPSIAIPAGYMESGRPFGITLASTAFSE
HHCCCEEEEEHCCCCEEEECHHHHHHHHHCCCCCCEEECCCHHCCCCCEEEEEECCCCCC
GTLIKLAYAFEQATKHRKIPGLS
CCEEEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA