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The map label for this gene is cls [H]

Identifier: 118477492

GI number: 118477492

Start: 1969859

End: 1971043

Strand: Direct

Name: cls [H]

Synonym: BALH_1816

Alternate gene names: 118477492

Gene position: 1969859-1971043 (Clockwise)

Preceding gene: 118477491

Following gene: 118477493

Centisome position: 37.47

GC content: 34.18

Gene sequence:

>1185_bases
ATGAAACACTTTTTCGTTGTAATCCTTTGTTTAATAGGTGTATTCATTTGGATGAACATCGATGTGGAAATGGGGAAAAA
AATGGCTAGTGAATATGAATTAGGACAAGTTCGATTGGGTGAATTTCAACTGTATACGAACGGTGAAGAATTATATACGA
AATTATTTGATGATATACGCAATGCAGAAAAGTACATATATATCCATTTTTATATTGTAGGTAAAGATGAAATAAGCAAA
GAGTTTTTGCAGTTGCTCCAGAAAAAGGCATCGGGTGGAGTAGAAGTGAAATTGTCAGTCGATCGAATAGGTGGATATAA
GCTGAGGAAAAAGGTAATTCGTCAACTGAAAAGCAATGGTGTTCAGTTTACATTTAGTAAAAAACCTAAACTGAAACATA
TATTCTACTCGTTGCATCAACGGAACCATAGACGCATTGTTACGATAGATGGAAAAGTATCATATATTGGTGGCTTTAAC
ATCGGCAAGGAATACCTTGGACAAAATCCGAAATTTGGTCCGTGGCGTGATTATCATGTACGAGTCAAGGGGAATGGTGC
CACGGATATGGAAAGAAAATTCTCTGCTGACTGGAAAGAAGATACGGGAGAAAAAATGCCTGTACATGAAAGTTTACCTG
CAATAGGGAATGTGAAATATCAATATTTATTTTCAGACGGAAAAGGTTTATGGGAAAAATATGGAGCGCTTATAAAGCAG
GCTAAAAAATCTTTAATTATTGCAACGCCATATTTTGTACCAAGTAAAGAAATGATAAAAGCGTTAAAAGATGCATTAAA
CCGCGGTGTCAATGTGAAAATTCTCGTACCATTTAAAAGTGATGCAGTATTGTTAAAACAAGCTGCCTATCCGTATTTGA
AAGACATGTTACATGCTGGAGCAGAGATTTATCAATATCAGAATGGTTTTTTTCATGGGAAAGTAACAATCATTGATGGG
GAAATTGTTGATATTGGTACCGCAAACTTTGATAATAGAAGTTTTTATTTAAATATGGAATCCAATTGTGTTATCTATGA
TAAGGGAGTAACAGCGGACGTATGGAGTCGATTAAAAGAAGACTTTCATAAATCAAAAAGATTTTCGGAAGAAGATTTTG
AGAAAATTAGTAAATGGGATTGGTTTTTAGCAAGAATAGCAAATGTTATAGCATCATATTTATAG

Upstream 100 bases:

>100_bases
ATATGGACATATATCATGCAATGACATATTTTCTTGAATGATATTGTATTAAAAAAGGGAAGATATAGAAATAACAGTAA
GTGGAGAAAGGAAAATATCC

Downstream 100 bases:

>100_bases
CATATAGACAAAAAGAAGGGAGGCACTTTCATGGATTGTATGAAAGAATTAATGAGGTATAGTTATATGCTTATATATAA
GGTTGGGGAGTATACAGGAG

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKHFFVVILCLIGVFIWMNIDVEMGKKMASEYELGQVRLGEFQLYTNGEELYTKLFDDIRNAEKYIYIHFYIVGKDEISK
EFLQLLQKKASGGVEVKLSVDRIGGYKLRKKVIRQLKSNGVQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYIGGFN
IGKEYLGQNPKFGPWRDYHVRVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGALIKQ
AKKSLIIATPYFVPSKEMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVTIIDG
EIVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Sequences:

>Translated_394_residues
MKHFFVVILCLIGVFIWMNIDVEMGKKMASEYELGQVRLGEFQLYTNGEELYTKLFDDIRNAEKYIYIHFYIVGKDEISK
EFLQLLQKKASGGVEVKLSVDRIGGYKLRKKVIRQLKSNGVQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYIGGFN
IGKEYLGQNPKFGPWRDYHVRVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGALIKQ
AKKSLIIATPYFVPSKEMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVTIIDG
EIVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL
>Mature_394_residues
MKHFFVVILCLIGVFIWMNIDVEMGKKMASEYELGQVRLGEFQLYTNGEELYTKLFDDIRNAEKYIYIHFYIVGKDEISK
EFLQLLQKKASGGVEVKLSVDRIGGYKLRKKVIRQLKSNGVQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYIGGFN
IGKEYLGQNPKFGPWRDYHVRVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGALIKQ
AKKSLIIATPYFVPSKEMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVTIIDG
EIVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=350, Percent_Identity=29.7142857142857, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787007, Length=320, Percent_Identity=26.5625, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45585; Mature: 45585

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFFVVILCLIGVFIWMNIDVEMGKKMASEYELGQVRLGEFQLYTNGEELYTKLFDDIR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECEEEEEECHHHHHHHHHHHHC
NAEKYIYIHFYIVGKDEISKEFLQLLQKKASGGVEVKLSVDRIGGYKLRKKVIRQLKSNG
CCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCC
VQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQNPKFGPWRDYHV
EEEEECCCCCHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCHHHCCCCCCCCCCCCEEE
RVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGALIKQ
EEECCCCCCHHHHHCCCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
AKKSLIIATPYFVPSKEMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAG
HHCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCHHHHHHHHCC
AEIYQYQNGFFHGKVTIIDGEIVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKE
HHHHHHCCCEEEEEEEEEECEEEEECCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHH
DFHKSKRFSEEDFEKISKWDWFLARIANVIASYL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHFFVVILCLIGVFIWMNIDVEMGKKMASEYELGQVRLGEFQLYTNGEELYTKLFDDIR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECEEEEEECHHHHHHHHHHHHC
NAEKYIYIHFYIVGKDEISKEFLQLLQKKASGGVEVKLSVDRIGGYKLRKKVIRQLKSNG
CCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCC
VQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYIGGFNIGKEYLGQNPKFGPWRDYHV
EEEEECCCCCHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCHHHCCCCCCCCCCCCEEE
RVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSDGKGLWEKYGALIKQ
EEECCCCCCHHHHHCCCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
AKKSLIIATPYFVPSKEMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAG
HHCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCHHHHHHHHCC
AEIYQYQNGFFHGKVTIIDGEIVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKE
HHHHHHCCCEEEEEEEEEECEEEEECCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHH
DFHKSKRFSEEDFEKISKWDWFLARIANVIASYL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]