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The map label for this gene is dacA [H]

Identifier: 118477407

GI number: 118477407

Start: 1880882

End: 1881835

Strand: Direct

Name: dacA [H]

Synonym: BALH_1727

Alternate gene names: 118477407

Gene position: 1880882-1881835 (Clockwise)

Preceding gene: 118477406

Following gene: 118477408

Centisome position: 35.78

GC content: 36.16

Gene sequence:

>954_bases
ATGAAGCGGTTAAAATGGGGAAGGATAACGATCCTATTAGTAATTATTTTAGGGATATGTTGGTTCTTATTCCAAGGAAC
TCAAAAGAGTGTAACAAATGTAGAGGGACAACCTTTACAAGTGGCAGAACTACCTAAAACAGGATTAACTGAAAAAGTAG
TGCCACCAAAGTATATACCACCGGAAGTTGATGCAAAAGCAGCTATGATTATTGATGCGAGTAATGGAGATATTATTTAT
CAACATAATGAAAATGAGGCTTTTGCGCCAGCAAGTATGTCTAAGATGATGACAGCATATCTTCTATTAGAAAATGTACA
TAATGGGAAAGTTCGCTGGGAAGATCCGGTGAAAATGAGTGCGAAAGCGGCACAAACAGAAGGGGCAAGAATCCCAGTCC
AAGTGAATGACACATTGACTGTTAAAGATTTGTTCCATGCATTAATGATTGAATCAGCAAATAATTCGGCTGTAGCTTTA
GCAGAACATATGGCAAAGACGGAGAAAGATTTCGTTCAGCTTATGAATGCAAAAGCAAAGCAATTAAAAATGTCGGATTA
TGCAAAATTCGCAAATGCATCAGGACTACAGGAGCCCGATGGAAGTGAGACGAAGATGACAGCTGCTGATGTAGCAAACT
TGGCGTATCATCTTATAAAAGATTACCCAGAAATATTAGAAGTGACTCACTTACGTCAAAGTCAGTTAGCATTTAATAAT
ATTAATGTTATAAGTACAAATGATATGTTAAACAAAAATAATAAAGCTTTATATATAGAAGGAATAGACGGATTAAAAAC
AGGATTCACAGATAGCGCAGGGTATTGTTTTACGGGTACAGCAAAACAAGGTGATACTCGTATTATTACGGTTGTAATGG
GAACGAAAGGTAAAACGAAACGTTTTACAGAGACAAATAAGCTAATGACTTATGCATTTGGCTTAGTTAATTAA

Upstream 100 bases:

>100_bases
GTTATAGATGAATAAATAGACTTTTTATCTTGACGATATAAAGGGAAAGTTGTTATGGTTGAATGTGAGTGTATAAAAGG
TTTGCTAAAGGAGTTTTGAA

Downstream 100 bases:

>100_bases
GTGAAATACTGAGTTGGATCATCATTATATGAAAGGCGATTTATTGTTATGTATTATCAGTAACAATAAATCGCCTTTTT
ATTTTTAATAGTAGGAAATA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIPPEVDAKAAMIIDASNGDIIY
QHNENEAFAPASMSKMMTAYLLLENVHNGKVRWEDPVKMSAKAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVAL
AEHMAKTEKDFVQLMNAKAKQLKMSDYAKFANASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNN
INVISTNDMLNKNNKALYIEGIDGLKTGFTDSAGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN

Sequences:

>Translated_317_residues
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIPPEVDAKAAMIIDASNGDIIY
QHNENEAFAPASMSKMMTAYLLLENVHNGKVRWEDPVKMSAKAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVAL
AEHMAKTEKDFVQLMNAKAKQLKMSDYAKFANASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNN
INVISTNDMLNKNNKALYIEGIDGLKTGFTDSAGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN
>Mature_317_residues
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIPPEVDAKAAMIIDASNGDIIY
QHNENEAFAPASMSKMMTAYLLLENVHNGKVRWEDPVKMSAKAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVAL
AEHMAKTEKDFVQLMNAKAKQLKMSDYAKFANASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNN
INVISTNDMLNKNNKALYIEGIDGLKTGFTDSAGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=259, Percent_Identity=33.5907335907336, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082022, Length=275, Percent_Identity=29.8181818181818, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=30.5343511450382, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87082054, Length=258, Percent_Identity=29.0697674418605, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 34996; Mature: 34996

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
PEVDAKAAMIIDASNGDIIYQHNENEAFAPASMSKMMTAYLLLENVHNGKVRWEDPVKMS
CCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHH
AKAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVALAEHMAKTEKDFVQLMNAKAK
HHHHCCCCCEEEEEECCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLKMSDYAKFANASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNN
HHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
INVISTNDMLNKNNKALYIEGIDGLKTGFTDSAGYCFTGTAKQGDTRIITVVMGTKGKTK
EEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCC
RFTETNKLMTYAFGLVN
CHHHHHHHEEHHHCCCC
>Mature Secondary Structure
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
PEVDAKAAMIIDASNGDIIYQHNENEAFAPASMSKMMTAYLLLENVHNGKVRWEDPVKMS
CCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHH
AKAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVALAEHMAKTEKDFVQLMNAKAK
HHHHCCCCCEEEEEECCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLKMSDYAKFANASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNN
HHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
INVISTNDMLNKNNKALYIEGIDGLKTGFTDSAGYCFTGTAKQGDTRIITVVMGTKGKTK
EEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCC
RFTETNKLMTYAFGLVN
CHHHHHHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]