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The map label for this gene is brnQ [H]

Identifier: 118477000

GI number: 118477000

Start: 1441484

End: 1442878

Strand: Reverse

Name: brnQ [H]

Synonym: BALH_1298

Alternate gene names: 118477000

Gene position: 1442878-1441484 (Counterclockwise)

Preceding gene: 118477001

Following gene: 118476990

Centisome position: 27.45

GC content: 37.63

Gene sequence:

>1395_bases
GTGTTCTTACAAATGAATGTAGAAAAGGAGGAAAAAACAATGAACACTGTATCAAAAAAACATATTTTTTTCACGGGTCT
TATGCTATTTTCTTTATTTTTTGGGGCAGGTAATTTAATTTTCCCTCCCATGCTTGGACAAAATGCAGGTGAAAACTTTT
GGCCGGCAATGATTGGATTTTTACTAACAGGAGTCGGTTTACCACTACTAACTGTTATCGCAATTTCTCTATCAGGAAAT
GGTATGCAACAACTTGCAAGTCATGTTCACCCATTATTCGGAATATTCTTTACGGTAGTTGTATACATTGCCATCGGTCC
TTCTATGGGTATTCCACGCGTTGCAAATGTCGCTTACGAAATGGGCGTTAGTTCTTTCTTACCAGAAACAATCCGTTCTA
GCAGCCTAGTGCTATTTGTATATACAATCATATTCTTTGCTATCGTATTTTGGCTGAGCTTGAATCCATCTAAACTTGTA
GATCGCATTGGAAATATATTAACACCTATTTTATTACTCTCTATTTTCTTATTGTTTGTTAAAAGTGTATTTACACCACT
CGGACAATCAGGACCAGCGATGCAAGAGTATCAAACTTCTCCAATCTTCAAAGGCTTTATGGAAGGATACTTAACGATGG
ATACCATTTCAGCACTTGCATTTGGAATTATCGTTGTAAATGCTATTCGCTCTAAAGGTGTGAATGATCGTAAATCCATT
GCTATCGCAACAGCAAAAGCAGGACTTATTGCCGCTACTGGTCTCGTACTTGTATATGGTGCGCTTGGCTGGCTCGGTAC
AACTAGCGTCTCGCTCGGATATGCAAAAAATGGCGGACAGTTACTTACTGTTATCGTACAACAATTATTCGGTCCATACG
GCTTACTTCTACTAGCTCTAATCGTTACACTCGCTTGCTTAACGACATGTGTTGGACTCGTATCTGCATGTAGTCAATAT
TTCTCAACATTATTGACTAAATTTTCATACAAAACATTTGCAAGTATCATTTGTGCTTTAGGGTTACTAGTTGCTAACTT
AGGTTTAACAAAAATCATTGCCATCTCTGTTCCAATTCTACTTGTTGTATATCCAATTGCAATCGTACTTGTTCTACTAT
CGTTACTGCATAAATACTTCGGGGGATATCGTTCTGTTTATGTAGGTGCTTTAATTGGAGCAGCTATAGTGAGCGTATTT
GATGGATTAAAACAAGGGGGTATTTCAGTTTCATTTATCACATCATATTTTGAATTTATCCCTTTATATAATGAAGGAAT
TGGCTGGCTAATACCTGCATTAGCCGGAGCTCTTATCGGCCTTACAATCGCTAAACTAAGCGGTGCGAAAAAAGTTCCTT
TACTAACCGAATCTCGTGAAGTAAAAGCATCCTAA

Upstream 100 bases:

>100_bases
TTGCAGAGCCGTATACATATAGAATCATTTCTACATGTATAAATTAGGGTGGTATCGCGGGTAAATATAACTCGTCCCTT
TCTTTAGGGACGAGTTTTTT

Downstream 100 bases:

>100_bases
AAAAACTCCCAAAAGGGAGTTTTTTTACTTTTGATAAGCAGTATGATTAAAACTGCTTATTTTATACGCTTTCGTATAAT
AGTCGATATTTGTATCCACT

Product: branched-chain amino acid transport protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MFLQMNVEKEEKTMNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGN
GMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLV
DRIGNILTPILLLSIFLLFVKSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSI
AIATAKAGLIAATGLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGLVSACSQY
FSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVF
DGLKQGGISVSFITSYFEFIPLYNEGIGWLIPALAGALIGLTIAKLSGAKKVPLLTESREVKAS

Sequences:

>Translated_464_residues
MFLQMNVEKEEKTMNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGN
GMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLV
DRIGNILTPILLLSIFLLFVKSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSI
AIATAKAGLIAATGLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGLVSACSQY
FSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVF
DGLKQGGISVSFITSYFEFIPLYNEGIGWLIPALAGALIGLTIAKLSGAKKVPLLTESREVKAS
>Mature_464_residues
MFLQMNVEKEEKTMNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGN
GMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLV
DRIGNILTPILLLSIFLLFVKSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSI
AIATAKAGLIAATGLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGLVSACSQY
FSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVF
DGLKQGGISVSFITSYFEFIPLYNEGIGWLIPALAGALIGLTIAKLSGAKKVPLLTESREVKAS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=437, Percent_Identity=37.9862700228833, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49609; Mature: 49609

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLQMNVEKEEKTMNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGF
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHH
LLTGVGLPLLTVIAISLSGNGMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLLSIFLLFV
HHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSI
HHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHE
AIATAKAGLIAATGLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLAL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCHHHHHHHHH
IVTLACLTTCVGLVSACSQYFSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFITSYFEFI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PLYNEGIGWLIPALAGALIGLTIAKLSGAKKVPLLTESREVKAS
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFLQMNVEKEEKTMNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGF
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHH
LLTGVGLPLLTVIAISLSGNGMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLLSIFLLFV
HHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KSVFTPLGQSGPAMQEYQTSPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSI
HHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHE
AIATAKAGLIAATGLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLAL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCHHHHHHHHH
IVTLACLTTCVGLVSACSQYFSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFITSYFEFI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PLYNEGIGWLIPALAGALIGLTIAKLSGAKKVPLLTESREVKAS
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]