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The map label for this gene is nrgA [H]

Identifier: 118476740

GI number: 118476740

Start: 1170069

End: 1171301

Strand: Reverse

Name: nrgA [H]

Synonym: BALH_1020

Alternate gene names: 118476740

Gene position: 1171301-1170069 (Counterclockwise)

Preceding gene: 118476743

Following gene: 118476736

Centisome position: 22.28

GC content: 43.8

Gene sequence:

>1233_bases
ATGAATATGGGAGATACGGTTTTTATGTTTGTAGCGACAGTAATGGTCATGTTAATGACACCAGGATTAGCACTTTTTTA
TGGAGGGATGGTTCGCAGTAAAAACGTACTCAGTACGACAATGCATAGTTATAGCGCAATGGCTATCGTTTCGATTCAAT
GGATTGTAATTGGTTATTCTTTATCATTCGGACCAGATTGGCACGGACTTATCGGCACACTCGATTGGTTCGGTTTAAAT
GGAGTTACCTACGCACCAAATCCAGACTACTCATCTACTATTCCTCACAATTTATTTATGATGTTTCAACTCATGTTCGC
TATTTTAACTCCTGCTTTAATTTCAGGTGCATTTGCCGAAAGAATGCGATTTTCAGCTTTCCTTATTTTCATCCTTCTAT
GGACAACGATCGTTTACAATCCTGTCGCTCATTGGGTATGGGGCGTTGGCGGATGGCTAAGAGAACTTGGCGCGTTAGAC
TTCGCTGGCGGTAATGTCGTTCATATTACATCTGGTGTGGCTGGCCTCGTATTAGCTATTTTCCTTGGAAAACGAAAAAA
CATAAACGGTACTTCTCCTCATCATTTACCATTTACGATGCTAGGCGCTGGGCTGCTATGGTTCGGTTGGTTCGGTTTTA
ACGTCGGAAGCGCATTATCTTTAAATGATGTAGCTTTAACAGCATTTATTAATACGAATATAGCCGCTGCTGCCTCCGCA
TTAACTTGGATGCTCTCAGAGTGGTTCTTTCAATCAAAACCGACTGCGATGGGAGCTGCTTGTGGCGCCGTTTCCGGTCT
AGTCGCTATTACACCAGCTTGCGGGTTCGTTACACCTTTTGCCGCTCTTATTATCGGAGCGATTGGCGGCGTATTATGCT
TCGGAGCAGTCTTTTTCTTAAAAACAAAATTCGGATACGACGATACACTCGATGCATTCGGATGTCACGGCATCGGCGGA
ACTTGGGGCGGAATTGCGACAGGACTATTTGCAACTACTGCCGTAAACGGCGATGGCGCTAACGGATTATTTTACGGAAA
CGCCGCGTTGCTGTTTAAACAACTTGTAGCAATCGGCGCAACATATGCATTCACAATTATAATGACGTACGCCATTATTA
AAGCAATCAACTTCTTCCTCCCTGTCCGAGTCGATGAGCATGAGGAACATATGGGACTTGATATTTCGATGCATGGGGAG
AAAGCTTATGAGTATACTGAGCGAGTGAACTAA

Upstream 100 bases:

>100_bases
AATTTTCTGAATTTTTAGTTGATTTTTCTTTTCACTTCGAATATGATATGAAACATAACAAATTGATGTTATTTTTTCTA
ACATCATAGGAGGAGAGAGA

Downstream 100 bases:

>100_bases
AAAAAGAGATGCAATCTATTGATAGTAGATTGCATCTCTTTTTTGCATTTATCGGCACTGCATACCCTAAGCGGTATTAC
TGTAAATATATCAGCGATTC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFAALIIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTAVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Sequences:

>Translated_410_residues
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFAALIIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTAVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN
>Mature_410_residues
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFAALIIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTAVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=42.997542997543, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=28.9044289044289, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=28.4705882352941, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=27.3148148148148, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=28.1464530892449, Blast_Score=131, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=39.9527186761229, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=412, Percent_Identity=36.8932038834951, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=36.8421052631579, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=30.2884615384615, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44068; Mature: 44068

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFAALIIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTAVNGDGANGLFYGNAALLFKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHCCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFAALIIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTAVNGDGANGLFYGNAALLFKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHCCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]