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The map label for this gene is brnQ [H]

Identifier: 118476371

GI number: 118476371

Start: 728099

End: 729424

Strand: Direct

Name: brnQ [H]

Synonym: BALH_0629

Alternate gene names: 118476371

Gene position: 728099-729424 (Clockwise)

Preceding gene: 118476369

Following gene: 118476372

Centisome position: 13.85

GC content: 35.44

Gene sequence:

>1326_bases
ATGAAATTAAAGAAGGAGAAAGAAATGAAATTGTTACAGAAAAAAGAAATTTTACTTATTAGTCTTATGTTATTCTCGAT
GTTCTTTGGAGCGGGAAATCTTATATTCCCACCTTTTCTTGGTTATGAAGCAGGAGAACATGTGTGGATTTCATTAGTAG
GGTTTATTATATCGGCAACAGGTCTTCCTATATTGGGAGTTATTGCGATTGCGAAAGCAGGAAGTTTCCAAACGTTAGCG
GGTAGGGTTCATTCCTCATTTGCAATTATTTTTCCGTGTATCGTGTACTTGTTTATTGGGCCAGGACTTGGTATACCACG
TGCAGGAAGTTTAGCTTTTGAAATGGGTCCAGGTCAGTTCTTCCCAGAAGCGGGAAGCATAGTTTTATTATGTTATACGG
TTATTTTCTTTAGTATCGTGTACTGGTTAAGCTTATCACCGTCTAAATTAATGGGGTTGTTCGGAAAGGTTTTAACACCA
TTATTACTAGGTATGATTGCCCTTATCTTTATAAAAAGCATGTTTACATCAGTAGGTAGTGTGAAAGAGGCTAGCGGAAA
CTATGGACAAGCGCCTATGTTCCAAGGATTTTTAGATGGCTATTTAACGATGGACGCATTAGCAGCTTTAATTTTTGGGA
TTGTGATTGCAAATGCTCTTCGTGCAAAAGGTATTGAAGATGATAAAGGTTTAGCGAAATATATGAGTATTGCTGGAATT
GGTGCAGGACTATTATTATCTATTATTTATGTCATACTTGGATATGTTGGTTCAATGAGTGGTTCATTGGGGATATTTGA
TAATGGCGCGCAAGTGTTGGCACAAGTGATGACTACACTATTTGGACAAGGCGGAGTCGTTTTATTAGGACTTATTTTTA
CGATCGCATGTTTATGTGTTTCAATCGGACTTGTTACATCGTGTAGTCAATTTTTCGCAAGTGCATTTCCGAAAGTAGCA
TACAAAGTTTGGGCATTTATCTTAAGTTTTGTTAGTATGATTTTAGCAAATTTAGGATTAACACAAATTTTAAAAGTATC
GGTACCAATTCTTGGATTTATTTATCCGGTTGCATTAACGCTTATTATTTTAGGGTTATTCCATAAGTATATTGGAAAGT
ATGCATACGTATATGCAGTGACAGTTGGGATTGTAGCCGTATTTAGTGCAATCGATATTTTCAATAAAAATGTAATGTTG
AATCAATGGACATCAGTATTAAAGTACATTCCGTTTTATACAGAAGGTGTAGGATGGATTGTTCCAGCTATTATAGGTGC
TTGTATTGGTGTTATCGTTAGTTTTGTTTTCAATAAGAAGAAATAA

Upstream 100 bases:

>100_bases
AGAATGAGTGTTTCTTTTATGTTAGACTATGGACTATGTTTTCTTTGTAAGGAAGCGGTTTATTGCACTTTTTTTGTATG
TGAAATTGATTTCATAAAAG

Downstream 100 bases:

>100_bases
GTAAGAGGTGTTTTCCTATTTCGGAAAACACCTTTCTTTTTTTGGAAAAATAGTATTGAAAATGAATAGACTAAGAGTAT
GATAAAGGTAATTGGTAATA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKLKKEKEMKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISATGLPILGVIAIAKAGSFQTLA
GRVHSSFAIIFPCIVYLFIGPGLGIPRAGSLAFEMGPGQFFPEAGSIVLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTP
LLLGMIALIFIKSMFTSVGSVKEASGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGI
GAGLLLSIIYVILGYVGSMSGSLGIFDNGAQVLAQVMTTLFGQGGVVLLGLIFTIACLCVSIGLVTSCSQFFASAFPKVA
YKVWAFILSFVSMILANLGLTQILKVSVPILGFIYPVALTLIILGLFHKYIGKYAYVYAVTVGIVAVFSAIDIFNKNVML
NQWTSVLKYIPFYTEGVGWIVPAIIGACIGVIVSFVFNKKK

Sequences:

>Translated_441_residues
MKLKKEKEMKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISATGLPILGVIAIAKAGSFQTLA
GRVHSSFAIIFPCIVYLFIGPGLGIPRAGSLAFEMGPGQFFPEAGSIVLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTP
LLLGMIALIFIKSMFTSVGSVKEASGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGI
GAGLLLSIIYVILGYVGSMSGSLGIFDNGAQVLAQVMTTLFGQGGVVLLGLIFTIACLCVSIGLVTSCSQFFASAFPKVA
YKVWAFILSFVSMILANLGLTQILKVSVPILGFIYPVALTLIILGLFHKYIGKYAYVYAVTVGIVAVFSAIDIFNKNVML
NQWTSVLKYIPFYTEGVGWIVPAIIGACIGVIVSFVFNKKK
>Mature_441_residues
MKLKKEKEMKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISATGLPILGVIAIAKAGSFQTLA
GRVHSSFAIIFPCIVYLFIGPGLGIPRAGSLAFEMGPGQFFPEAGSIVLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTP
LLLGMIALIFIKSMFTSVGSVKEASGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGI
GAGLLLSIIYVILGYVGSMSGSLGIFDNGAQVLAQVMTTLFGQGGVVLLGLIFTIACLCVSIGLVTSCSQFFASAFPKVA
YKVWAFILSFVSMILANLGLTQILKVSVPILGFIYPVALTLIILGLFHKYIGKYAYVYAVTVGIVAVFSAIDIFNKNVML
NQWTSVLKYIPFYTEGVGWIVPAIIGACIGVIVSFVFNKKK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=426, Percent_Identity=35.2112676056338, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47340; Mature: 47340

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKEKEMKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GLPILGVIAIAKAGSFQTLAGRVHSSFAIIFPCIVYLFIGPGLGIPRAGSLAFEMGPGQF
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
FPEAGSIVLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLGMIALIFIKSMFTSVGS
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKEASGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGI
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GAGLLLSIIYVILGYVGSMSGSLGIFDNGAQVLAQVMTTLFGQGGVVLLGLIFTIACLCV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SIGLVTSCSQFFASAFPKVAYKVWAFILSFVSMILANLGLTQILKVSVPILGFIYPVALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LIILGLFHKYIGKYAYVYAVTVGIVAVFSAIDIFNKNVMLNQWTSVLKYIPFYTEGVGWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCHHHH
VPAIIGACIGVIVSFVFNKKK
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]