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The map label for this gene is thiM [H]

Identifier: 118476129

GI number: 118476129

Start: 432183

End: 432998

Strand: Direct

Name: thiM [H]

Synonym: BALH_0374

Alternate gene names: 118476129

Gene position: 432183-432998 (Clockwise)

Preceding gene: 118476127

Following gene: 118476130

Centisome position: 8.22

GC content: 40.2

Gene sequence:

>816_bases
ATGAGAAGGATGAATATGAAAGAAATTAGCAAAGTAGTGGATTTGGTGAGAGAATCTAATCCGCTCGTTCATAATATTAC
AAATGTTGTTGTAACAAATTTTACAGCTAACGGTTTATTAGCATTAGGGGCATCCCCTGTAATGGCGTATGCAAAAGAAG
AAGTAGCAGAAATGGCTAGCATTGCTGGAGCGTTAGTATTAAATATGGGGACACTTCGTCCTGACGAGGTAGAAGCGATG
TTACTTGCTGGTAAGTCGGCTAATAGGAATGATGTACCGGTACTGTTTGATCCGGTTGGCGCAGGGGCAACTTCGTATCG
AACAGAAGTTGCGAGACATATTCCGGCTGAAATCGAATTAGCAATTATTCGCGGAAATGCAGCTGAAATAGCGAACGTTA
TTAATGAAAAATGGGAAATTAAAGGGGTAGACGCTGGTGCTGGAAATGGTAATGTCGTAAGTATTGCAAAGCAGGCAGCA
GATGAACTAAATACAGTTGCGGTGATTACTGGAAAAGAAGATGTTGTTACAGATGGAGAGCGAACTATTGTTATTCGAAA
TGGTCACTCTATTTTAACGAAAATTACGGGAACTGGTTGTTTACTAACTTCTGTAATAGGAGCATTTGTAGCGGTAGAAA
AGGATTATGTAAAGGCTGCAGTAGCGGCATTAACGTTTTATGGTGTAGCCGCGGAACTAGCAGCTGCTAAGACAGTAGAA
AAAGGACCTGGTAGTTTCCAAATTGAATTTTTAAATCAGTTAGCCAATACGACTTCGGGTGATATAGAAAAGTATGGGAA
GATTGAGGTTATATAG

Upstream 100 bases:

>100_bases
GGATAATGCCAGCGTAGGGAGAGTGTAAAAGCACATGTTCAGGCACTTTGCGTACGCGCAAAGTGCCTTTTTGTGTATCT
CCCATCACTATAGTTAGGAG

Downstream 100 bases:

>100_bases
TAAGGAGGGAAAATAAATGTCACGTATTTCAAAGGCAGAAATGTCTAAGTTATTATCAGTATATTTTATTATGGGAAGTA
ACAATTGTACGAAGGATCCA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MRRMNMKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAM
LLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAA
DELNTVAVITGKEDVVTDGERTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVE
KGPGSFQIEFLNQLANTTSGDIEKYGKIEVI

Sequences:

>Translated_271_residues
MRRMNMKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAM
LLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAA
DELNTVAVITGKEDVVTDGERTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVE
KGPGSFQIEFLNQLANTTSGDIEKYGKIEVI
>Mature_271_residues
MRRMNMKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAM
LLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIELAIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAA
DELNTVAVITGKEDVVTDGERTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVE
KGPGSFQIEFLNQLANTTSGDIEKYGKIEVI

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=254, Percent_Identity=39.3700787401575, Blast_Score=160, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=276, Percent_Identity=34.0579710144928, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28533; Mature: 28533

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRMNMKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHH
IAGALVLNMGTLRPDEVEAMLLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIEL
HHHHHHHHCCCCCCHHHHEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEE
AIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADELNTVAVITGKEDVVTDGE
EEEECCHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCC
RTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVE
EEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGPGSFQIEFLNQLANTTSGDIEKYGKIEVI
CCCCCEEHHHHHHHHCCCCCCHHHCCCEEEC
>Mature Secondary Structure
MRRMNMKEISKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHH
IAGALVLNMGTLRPDEVEAMLLAGKSANRNDVPVLFDPVGAGATSYRTEVARHIPAEIEL
HHHHHHHHCCCCCCHHHHEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEE
AIIRGNAAEIANVINEKWEIKGVDAGAGNGNVVSIAKQAADELNTVAVITGKEDVVTDGE
EEEECCHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCC
RTIVIRNGHSILTKITGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVAAELAAAKTVE
EEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGPGSFQIEFLNQLANTTSGDIEKYGKIEVI
CCCCCEEHHHHHHHHCCCCCCHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA