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The map label for this gene is purF [H]

Identifier: 118476046

GI number: 118476046

Start: 331807

End: 333240

Strand: Direct

Name: purF [H]

Synonym: BALH_0289

Alternate gene names: 118476046

Gene position: 331807-333240 (Clockwise)

Preceding gene: 118476045

Following gene: 118476047

Centisome position: 6.31

GC content: 40.1

Gene sequence:

>1434_bases
TTGGAAAGGGGCAATCCCATGCTTGCTGAAATAAAGGGGTTAAACGAAGAATGTGGCGTTTTCGGAATTTGGGGGCATGA
AAATGCGGCACAAGTTTCATACTACGGATTGCACAGTTTACAGCATCGTGGGCAAGAAGGCGCAGGCATTGTCGTAAATA
ATGGGGAAAAAATCGTCGGTCACAAGGGGTTAGGTTTAATATCGGAAGTGTTTTCAAGAGGTGAGCTAGAAGGATTGAAT
GGAAAATCAGCAATCGGGCATGTACGATACGCGACGGCTGGTGGAAGTGAAGTGGCTAACGTTCAACCATTACTATTCCG
TTTTTCTGATCATAGTATGGCATTAGCTCATAACGGGAATTTAATTAATGCAAAAATGCTTCGCCGCGAATTAGAGGCAG
AGGGAAGTATTTTTCAAACAAGTTCAGATACAGAAGTACTTTTACATCTTATTAAACGTAGTACGAAAGATTCGTTAATT
GAAAGTGTAAAAGAGGCTTTAAATAAAGTGAAAGGTGCGTTTGCGTATCTTTTACTAACTGGAAATGAAATGATCGTTGC
GTTAGATCCGAATGGTTTCCGTCCTCTTTCAATTGGAAAGATGGGTGATGCTTACGTTGTAGCATCAGAAACATGTGCTT
TCGATGTAGTAGGTGCAACATACATTCGTGATGTAGAACCGGGTGAATTACTTATCATCAATGATGAAGGAATTCACGTA
GATCGTTTTACAAATGAAGTAGATCATGCAATTTGTAGTATGGAATACATTTACTTTGCACGACCAGATTCTAATATTGC
TGGTATTAACGTCCATGCAGCACGTAAAAACATGGGAAAACGTTTGGCGGCAGAAGCTCCTATTGAAGCTGATGTTGTTA
CTGGTGTGCCAGACTCTAGTATTTCGGCAGCAATTGGTTATGCGGAGGCGACAGGTATTCCATATGAGTTAGGATTAATT
AAAAATCGTTACGTTGGACGTACATTTATTCAACCTTCTCAAGAACTGCGCGAGCAAGGGGTTAAGATGAAGCTTTCAGC
AGTAAGAGGGGTAGTTGAAGGAAAACGAGTTGTTATGATTGACGATTCTATCGTAAGAGGAACAACAAGTAAACGAATTG
TTCGCATGCTTCGCGAGGCTGGAGCGACAGAAGTTCACGTAAGAATTGCTTCACCACCTCTGAAATATCCATGCTTCTAT
GGTATTGATATTCAAACGAGAAAAGAATTAATTGCAGCAAATCATACGGTAGAAGAAATTCGTGAAATGATCGGTGCAGA
CTCATTAACATTTTTAAGTGAAGATGGATTAGTAGATGCAATTGGACGTCCATATGAAGGGAAATATGGCGGCCTATGTA
TGGCTTACTTTAATGGGGACTATCCAACAGCTCTTTATGATTATGAGCAAGAGCTTTTAGAAAGTATGAAATAA

Upstream 100 bases:

>100_bases
CAATTCAAGTTGGAGAAGTAACAAATACAAATGAAGTAACAATTCATAATGAAGAAAATGAAGTATTACTTACAGCAAAT
GTAGATGAAATGAGAAAGGC

Downstream 100 bases:

>100_bases
GAAAAATGAAAAAAGCTTCTACTTCTTTTGATGTAGAAGCTAGCTTCTTTTTTATAAATAGCCCGCCCTGGATGGGGAGA
AATTAAAAGACGAGGTGTAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MERGNPMLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGLISEVFSRGELEGLN
GKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLI
ESVKEALNKVKGAFAYLLLTGNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIHV
DRFTNEVDHAICSMEYIYFARPDSNIAGINVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIGYAEATGIPYELGLI
KNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFY
GIDIQTRKELIAANHTVEEIREMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK

Sequences:

>Translated_477_residues
MERGNPMLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGLISEVFSRGELEGLN
GKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLI
ESVKEALNKVKGAFAYLLLTGNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIHV
DRFTNEVDHAICSMEYIYFARPDSNIAGINVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIGYAEATGIPYELGLI
KNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFY
GIDIQTRKELIAANHTVEEIREMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
>Mature_477_residues
MERGNPMLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVGHKGLGLISEVFSRGELEGLN
GKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGNLINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLI
ESVKEALNKVKGAFAYLLLTGNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIHV
DRFTNEVDHAICSMEYIYFARPDSNIAGINVHAARKNMGKRLAAEAPIEADVVTGVPDSSISAAIGYAEATGIPYELGLI
KNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFY
GIDIQTRKELIAANHTVEEIREMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=463, Percent_Identity=40.1727861771058, Blast_Score=345, Evalue=7e-95,
Organism=Escherichia coli, GI1788651, Length=487, Percent_Identity=40.2464065708419, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1790167, Length=207, Percent_Identity=29.951690821256, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=39.9168399168399, Blast_Score=337, Evalue=9e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=493, Percent_Identity=36.105476673428, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=41.4486921529175, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659598, Length=500, Percent_Identity=39.8, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=41.036717062635, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52090; Mature: 52090

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGNPMLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVG
CCCCCCCHHEECCCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCCCCCEEEEECCCEEEC
HKGLGLISEVFSRGELEGLNGKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGN
CCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCC
LINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEALNKVKGAFAYLLLT
EEEHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEE
GNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIHV
CCEEEEEECCCCCCEEEECCCCCEEEEEECCCEEEEECHHHEECCCCCCEEEECCCCEEE
DRFTNEVDHAICSMEYIYFARPDSNIAGINVHAARKNMGKRLAAEAPIEADVVTGVPDSS
HHHHHHHHHHHHCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCC
ISAAIGYAEATGIPYELGLIKNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMI
CHHHCCHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEE
DDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTVEEI
CCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCHHHH
REMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
HHHHCCCCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MERGNPMLAEIKGLNEECGVFGIWGHENAAQVSYYGLHSLQHRGQEGAGIVVNNGEKIVG
CCCCCCCHHEECCCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCCCCCEEEEECCCEEEC
HKGLGLISEVFSRGELEGLNGKSAIGHVRYATAGGSEVANVQPLLFRFSDHSMALAHNGN
CCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCC
LINAKMLRRELEAEGSIFQTSSDTEVLLHLIKRSTKDSLIESVKEALNKVKGAFAYLLLT
EEEHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEE
GNEMIVALDPNGFRPLSIGKMGDAYVVASETCAFDVVGATYIRDVEPGELLIINDEGIHV
CCEEEEEECCCCCCEEEECCCCCEEEEEECCCEEEEECHHHEECCCCCCEEEECCCCEEE
DRFTNEVDHAICSMEYIYFARPDSNIAGINVHAARKNMGKRLAAEAPIEADVVTGVPDSS
HHHHHHHHHHHHCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCC
ISAAIGYAEATGIPYELGLIKNRYVGRTFIQPSQELREQGVKMKLSAVRGVVEGKRVVMI
CHHHCCHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEE
DDSIVRGTTSKRIVRMLREAGATEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTVEEI
CCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCHHHH
REMIGADSLTFLSEDGLVDAIGRPYEGKYGGLCMAYFNGDYPTALYDYEQELLESMK
HHHHCCCCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]