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The map label for this gene is truA [H]

Identifier: 118475910

GI number: 118475910

Start: 143927

End: 144721

Strand: Direct

Name: truA [H]

Synonym: BALH_0140

Alternate gene names: 118475910

Gene position: 143927-144721 (Clockwise)

Preceding gene: 118475909

Following gene: 118475911

Centisome position: 2.74

GC content: 36.73

Gene sequence:

>795_bases
TTGTTTAGCTTGTATTTTGGCATGGGTGCGATCGTAATGGAGAATAGAGAAATGGATAGAATAAAATGTACGGTTGCATA
TGATGGCATGCATTTTTGTGGTTATCAAATTCAACCGCAGCATCGTACTGTTCAACAAGAGATAGAGAAAGCTTTGCAGA
AATTGCATAAAGGAGAACTTGTTCGTGTTCAGGCATCGGGCAGAACGGATTCTACCGTTCATGCTAAAGGACAAGTGATA
CACTTTGATACACCGTTGTCTCTTGAAGAGTGGCAATGGAGTAATGCATTAAACACAATGCTACCAGATGATATTGTTAT
CACACAGGTAGAGAAAAAAACAGAAGAATTTCATGCTCGATACGGTGTTGAGAGAAAAGAATATCGTTATCGTGTACTAG
TATCAAAGACTGCGGATGTATTCCGTAGAAATTATGTATATCAATATCCATATCCACTCGAAGTAAACTCTATAAGAAAA
GCGATCCCTTATTTTATTGGAACGCATGATTTCACTTCTTTTTGTTCGGCAAAAACTGACAAAAAAGATAAAGTGAGAAC
AATTTATGAAATCGAGCTAATTGAGCAAGACGATGAATTAATTTTTCGTTTTGTAGGGAATGGATTTTTATATAATATGG
TCAGAATTATTGTTGGTACGTTACTTAACGTAGGTCAAGGAAAGCTTGATCCGGATAGCATTCCAGAGATTTTAGCGAAA
CAAAATCGTCAGTTTGCTGGAAAGATGGCTCCTGGTCATGGGCTTTATTTATGGCAGGTAAACTATAACAACTAA

Upstream 100 bases:

>100_bases
TCGCTATGGAAGCTCGTGGATATCAAAGTGGTGAAGGACGTACTAAATTTAGGCAACTACGCTGGAAAACAAGCGATACA
GTAACGATTATAAGCTTACT

Downstream 100 bases:

>100_bases
TCCTGGTGTAACATTTGCTTGACATTAGAAACTCAAAAGTATATCATAACATATGGTATTGTTTCTAAAACCACGATTAG
CCCCGGAAGGAAATCGTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MFSLYFGMGAIVMENREMDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVI
HFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLVSKTADVFRRNYVYQYPYPLEVNSIRK
AIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLNVGQGKLDPDSIPEILAK
QNRQFAGKMAPGHGLYLWQVNYNN

Sequences:

>Translated_264_residues
MFSLYFGMGAIVMENREMDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVI
HFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLVSKTADVFRRNYVYQYPYPLEVNSIRK
AIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLNVGQGKLDPDSIPEILAK
QNRQFAGKMAPGHGLYLWQVNYNN
>Mature_264_residues
MFSLYFGMGAIVMENREMDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVI
HFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHARYGVERKEYRYRVLVSKTADVFRRNYVYQYPYPLEVNSIRK
AIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLNVGQGKLDPDSIPEILAK
QNRQFAGKMAPGHGLYLWQVNYNN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=249, Percent_Identity=30.5220883534137, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=32.0987654320988, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321112, Length=239, Percent_Identity=26.3598326359833, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007752, Length=269, Percent_Identity=25.6505576208178, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30750; Mature: 30750

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLYFGMGAIVMENREMDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGEL
CEEEEECCCEEEECCCCCCEEEEEEEECCEEECCEEECCHHHHHHHHHHHHHHHHCCCCE
VRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHAR
EEEEECCCCCCEEECCCEEEEECCCCCHHHHCHHHHHHHCCCCCEEEEHHHHHHHHHHHH
YGVERKEYRYRVLVSKTADVFRRNYVYQYPYPLEVNSIRKAIPYFIGTHDFTSFCSAKTD
HCCCHHHEEEEEEEHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCC
KKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLNVGQGKLDPDSIPEILAK
CCCHHEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QNRQFAGKMAPGHGLYLWQVNYNN
CCCHHCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MFSLYFGMGAIVMENREMDRIKCTVAYDGMHFCGYQIQPQHRTVQQEIEKALQKLHKGEL
CEEEEECCCEEEECCCCCCEEEEEEEECCEEECCEEECCHHHHHHHHHHHHHHHHCCCCE
VRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQWSNALNTMLPDDIVITQVEKKTEEFHAR
EEEEECCCCCCEEECCCEEEEECCCCCHHHHCHHHHHHHCCCCCEEEEHHHHHHHHHHHH
YGVERKEYRYRVLVSKTADVFRRNYVYQYPYPLEVNSIRKAIPYFIGTHDFTSFCSAKTD
HCCCHHHEEEEEEEHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCC
KKDKVRTIYEIELIEQDDELIFRFVGNGFLYNMVRIIVGTLLNVGQGKLDPDSIPEILAK
CCCHHEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QNRQFAGKMAPGHGLYLWQVNYNN
CCCHHCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]