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The map label for this gene is wcaJ [H]

Identifier: 117919825

GI number: 117919825

Start: 1592670

End: 1593659

Strand: Direct

Name: wcaJ [H]

Synonym: Shewana3_1377

Alternate gene names: 117919825

Gene position: 1592670-1593659 (Clockwise)

Preceding gene: 117919824

Following gene: 117919826

Centisome position: 32.03

GC content: 38.08

Gene sequence:

>990_bases
ATGAATATACGAGTTAATGAAACGGTTGACACTCATGAGTTTCAGCCGATTAAAACAGTTGCGAAGCGTTTCAAGTCAAA
GTATCAGGTGCTAAATCAGCTGCTTATTTATGTCTATTGTCCTGTGGATAGAGAATTAGAGATTGGTCAAATTATTGATG
ACTACAGCTTTGATATGGTTGTCAATGATTACAGCATTACTGATTTGAGTTGTAAGGTTATTTGTCATTTTGAAAGCGCG
CGTTTGACTAAAGAACAGAGACAGTTTCTTGTCAAAGCGACTGAACTTGGTGCTTGGGTAGAGCCTTTAGTTAGCTATCT
TGATGAAAGACTTGGCTATACTGAGGTCCGATTACTACATAGTGGCTATTTTCTACATCAGAAAGCTTTTTCCATTTTAT
CGACAAAGCGTACTCAATTAGCTAAAAGAGTTGTTGATTTATTTGCGGCGTTTCTGCTTTGTACTGTAGCAATACCTGTC
GGATTACTTACAGCATTGGCGATTAAGTTAGAAAGTCCAGGGCCAGTATTTTATAAACAGCGACGTACAGGACAATATAA
TGACGAATTTGAAGTGATTAAATTTCGTTCTATGCGTAATGATGCTGAAAAGTCTGGTGCGCAATGGGCTACTCAAAATG
ATGCTCGTGTTACAAAAGTAGGTAACTTTATTCGTAAGACCCGCATCGATGAATTACCGCAGATCATCAATATTCTGAAA
GGTGAAATGTCGATTGTTGGTCCGAGACCAGAGCGTGAGGTCTTTATTGCAGAATTAGAACAAGAAATTCCTTATTATCG
CTTCCGCCACGCAGTTAAACCCGGTGTAACGGGTCTCGCCCAAGTCAGTTATCCATACGGTGCTTCTGTTGAGGATGCGG
TTTGGAAGCATAAGTATGATATTTTTTATATTAAACACCACAGTACTTTATTAGATGTGAAAATTTTACTTAAAACAATT
AAAGTTGTTTTATTTGGCATGGGACGATAA

Upstream 100 bases:

>100_bases
GCTCGGCGGTATGTTAGGTTTGATGTTAGTGCTGGTAAGGCATTTTATGAGAAAAGAGTAAGCTCTAGATCCTAGACCCC
CTATTAATTTGGTTTAATCT

Downstream 100 bases:

>100_bases
ATGCAAGAGCAACCATTAGTATCGATTATCATGCCCAGCTATAACTCAGCTAAAACTATTGCTGAAAGTATAGAGTCAGT
TATCTCTCAAACATATCAAA

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNIRVNETVDTHEFQPIKTVAKRFKSKYQVLNQLLIYVYCPVDRELEIGQIIDDYSFDMVVNDYSITDLSCKVICHFESA
RLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKRVVDLFAAFLLCTVAIPV
GLLTALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWATQNDARVTKVGNFIRKTRIDELPQIINILK
GEMSIVGPRPEREVFIAELEQEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYDIFYIKHHSTLLDVKILLKTI
KVVLFGMGR

Sequences:

>Translated_329_residues
MNIRVNETVDTHEFQPIKTVAKRFKSKYQVLNQLLIYVYCPVDRELEIGQIIDDYSFDMVVNDYSITDLSCKVICHFESA
RLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKRVVDLFAAFLLCTVAIPV
GLLTALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWATQNDARVTKVGNFIRKTRIDELPQIINILK
GEMSIVGPRPEREVFIAELEQEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYDIFYIKHHSTLLDVKILLKTI
KVVLFGMGR
>Mature_329_residues
MNIRVNETVDTHEFQPIKTVAKRFKSKYQVLNQLLIYVYCPVDRELEIGQIIDDYSFDMVVNDYSITDLSCKVICHFESA
RLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLHSGYFLHQKAFSILSTKRTQLAKRVVDLFAAFLLCTVAIPV
GLLTALAIKLESPGPVFYKQRRTGQYNDEFEVIKFRSMRNDAEKSGAQWATQNDARVTKVGNFIRKTRIDELPQIINILK
GEMSIVGPRPEREVFIAELEQEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYDIFYIKHHSTLLDVKILLKTI
KVVLFGMGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=185, Percent_Identity=44.3243243243243, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 37966; Mature: 37966

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRVNETVDTHEFQPIKTVAKRFKSKYQVLNQLLIYVYCPVDRELEIGQIIDDYSFDMV
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHCCCCCEEE
VNDYSITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLH
EECCEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SGYFLHQKAFSILSTKRTQLAKRVVDLFAAFLLCTVAIPVGLLTALAIKLESPGPVFYKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEC
RRTGQYNDEFEVIKFRSMRNDAEKSGAQWATQNDARVTKVGNFIRKTRIDELPQIINILK
CCCCCCCCCHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GEMSIVGPRPEREVFIAELEQEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYD
CCCEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHCCCE
IFYIKHHSTLLDVKILLKTIKVVLFGMGR
EEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIRVNETVDTHEFQPIKTVAKRFKSKYQVLNQLLIYVYCPVDRELEIGQIIDDYSFDMV
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHCCCCCEEE
VNDYSITDLSCKVICHFESARLTKEQRQFLVKATELGAWVEPLVSYLDERLGYTEVRLLH
EECCEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SGYFLHQKAFSILSTKRTQLAKRVVDLFAAFLLCTVAIPVGLLTALAIKLESPGPVFYKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEC
RRTGQYNDEFEVIKFRSMRNDAEKSGAQWATQNDARVTKVGNFIRKTRIDELPQIINILK
CCCCCCCCCHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GEMSIVGPRPEREVFIAELEQEIPYYRFRHAVKPGVTGLAQVSYPYGASVEDAVWKHKYD
CCCEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHCCCE
IFYIKHHSTLLDVKILLKTIKVVLFGMGR
EEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]