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The map label for this gene is ushA [C]

Identifier: 117919673

GI number: 117919673

Start: 1426364

End: 1428115

Strand: Reverse

Name: ushA [C]

Synonym: Shewana3_1224

Alternate gene names: 117919673

Gene position: 1428115-1426364 (Counterclockwise)

Preceding gene: 117919674

Following gene: 117919671

Centisome position: 28.72

GC content: 50.97

Gene sequence:

>1752_bases
ATGACACAGGGATACACACTCTCACTCGCGCACATCAACGATACCCATTCGAATTTTGAGCCCAGCCGCGTACAATTTTC
CTTCAATCTCGGGCTTAAAGCCTTAGATATCACCAGTCACAGCGGTGGCTATGCGCGCCTGGGGTATCAGATTAGTCAGG
CACGTGACCGAGCCGCACAAAGCCAAATGCCGTTTTTATTTTTGCATGGGGGTGACAGTTTCCAAGGCACGCTGTATTTC
AGCCATTTTAAGGGTAAGGCCAACGCCCATTTGCTGAATTTACTCTCACCCGATGCCATGGTGATTGGTAATCACGATAT
TGATGAAGGCAATGCGCGGCTCGCCGAATTTGCTAAGCAAATCGATTTTCCGCTGCTGGCAGGCAATATGGACTTAAGCC
AAGAGGATATCCGCAAGCCGGGTAACCTGCGTACTGTGGCGAATATTCTGGCCTATGATGAAAAAAACCAACTTGCCAGC
TACCTCGAAAAGCCCTTCTACGACAAAAAGCTCGCTATCATCGGCATTACTCTGGATCAGATGAAAAACATCGCCAGACC
CGATCCCGACACTCACTTTGTCGATGCGATTGCGACCACGGCGCGCACAGTCGCGCATCTGCATGCCAAAGGCATCAAGC
ACATTATCGTACTGAGTCACTTAGGTTTAGATCAGGACAAAAGGCTTGCAGAACAAGTCGATGGCATCAGCCTTATCGTC
GGTGGCCATACCCATACATTGCAGGGCGATTTTAGTGCCTTGGGGTTAAGCAATATACCCTATGGTGAAACCATCCATGG
CACTCCTATCGTTCATGCGGGGAAATACGCCGAAACCTTAGGCTTGGCCGAGATTGAATTTGATGCAGCGGGCCGCGCGA
TAAGCCTAAAAGGTGGCAACTATTTTATGCTCGATAAGCAGTTTATGTTGAGTTCGAGTGAGCAAGTGGATGACACTGAC
TATCGAGCCGTGCAGCAACGCTTTAATGAGCATCCCTATGTATTGTGGGATGATGCCGATGAAGACATTCACGGTGTGAT
CCAAGATATCTATCGCCCCGCGATTGCCGAGATGGAAAACAAAGTCTTAGCCCTTGTGCCTAAGAATCTAGTGCATACCA
GGCTGCCTTCTAAGGCATTACCCCATGGCAGCGAAATCGCCCCTTGGGTGAGCCGCAGTATGTTCCAAGAGGCCAAACTT
GAAGATCCTCGCATCCACTTTGCCCTGCATAACGCGGGCGGAGTGAGACAATCTGTCGCCCAAGGCAGGCTCACCCTCGC
CGATGTGTTGGGCCAATTGCTCCCCTTCGAATTGCCATTAGTCAAATACGAAATCCAAGGCCAGTATTTATACCAAGCGC
TGGAATCGGCGATTAACGCCGCGACAAACAACAGTGTGCGCGGCACCGGTGCGGGCAGCTTCCCCTATACCTACGGATTG
CGTTATTACTACGATGGTAGACGCCCATTAGGGGAGCGTTTGTTCAAGCTTGAAATGATGCAGCAGGATGGCTTATCGCT
CTGGTATCCAGTGCAACCCAATGCACTCTATGTGGGGGTTTCTACCTCTTATACCGCTGCGGGGAAAGAAGGCTATCACC
CACTGTTACAGGCGCGCTGGCAGGAATCGATTGAGACGCTCACCCTACCCCAGGCCTTTATTCGCTTCCTCAATCGAGCG
CCCAACCCCAATTTAGCGGATTTGCTCTCGCCCCATGTGCATTACACCAGCCATGTAAAGCGCTCGGTGTAA

Upstream 100 bases:

>100_bases
TAATGACATTATTTAGTCATTGTTAAAGTAAAGGGAGCTTGGCTCCCTTTCTTGTTTTTGGGGAGAAGGTTATAATCTCC
CGCCGTAAAAGAGGTAGGTT

Downstream 100 bases:

>100_bases
GCGCACTGCCGCGAAGCTCTGTCCTCTTAACTCTGTCCTCTTAATTAATCACAGTACTTAGCCAATGGGTTAAGTACTGT
GATTAATTCAATCCACATCA

Product: metallophosphoesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MTQGYTLSLAHINDTHSNFEPSRVQFSFNLGLKALDITSHSGGYARLGYQISQARDRAAQSQMPFLFLHGGDSFQGTLYF
SHFKGKANAHLLNLLSPDAMVIGNHDIDEGNARLAEFAKQIDFPLLAGNMDLSQEDIRKPGNLRTVANILAYDEKNQLAS
YLEKPFYDKKLAIIGITLDQMKNIARPDPDTHFVDAIATTARTVAHLHAKGIKHIIVLSHLGLDQDKRLAEQVDGISLIV
GGHTHTLQGDFSALGLSNIPYGETIHGTPIVHAGKYAETLGLAEIEFDAAGRAISLKGGNYFMLDKQFMLSSSEQVDDTD
YRAVQQRFNEHPYVLWDDADEDIHGVIQDIYRPAIAEMENKVLALVPKNLVHTRLPSKALPHGSEIAPWVSRSMFQEAKL
EDPRIHFALHNAGGVRQSVAQGRLTLADVLGQLLPFELPLVKYEIQGQYLYQALESAINAATNNSVRGTGAGSFPYTYGL
RYYYDGRRPLGERLFKLEMMQQDGLSLWYPVQPNALYVGVSTSYTAAGKEGYHPLLQARWQESIETLTLPQAFIRFLNRA
PNPNLADLLSPHVHYTSHVKRSV

Sequences:

>Translated_583_residues
MTQGYTLSLAHINDTHSNFEPSRVQFSFNLGLKALDITSHSGGYARLGYQISQARDRAAQSQMPFLFLHGGDSFQGTLYF
SHFKGKANAHLLNLLSPDAMVIGNHDIDEGNARLAEFAKQIDFPLLAGNMDLSQEDIRKPGNLRTVANILAYDEKNQLAS
YLEKPFYDKKLAIIGITLDQMKNIARPDPDTHFVDAIATTARTVAHLHAKGIKHIIVLSHLGLDQDKRLAEQVDGISLIV
GGHTHTLQGDFSALGLSNIPYGETIHGTPIVHAGKYAETLGLAEIEFDAAGRAISLKGGNYFMLDKQFMLSSSEQVDDTD
YRAVQQRFNEHPYVLWDDADEDIHGVIQDIYRPAIAEMENKVLALVPKNLVHTRLPSKALPHGSEIAPWVSRSMFQEAKL
EDPRIHFALHNAGGVRQSVAQGRLTLADVLGQLLPFELPLVKYEIQGQYLYQALESAINAATNNSVRGTGAGSFPYTYGL
RYYYDGRRPLGERLFKLEMMQQDGLSLWYPVQPNALYVGVSTSYTAAGKEGYHPLLQARWQESIETLTLPQAFIRFLNRA
PNPNLADLLSPHVHYTSHVKRSV
>Mature_582_residues
TQGYTLSLAHINDTHSNFEPSRVQFSFNLGLKALDITSHSGGYARLGYQISQARDRAAQSQMPFLFLHGGDSFQGTLYFS
HFKGKANAHLLNLLSPDAMVIGNHDIDEGNARLAEFAKQIDFPLLAGNMDLSQEDIRKPGNLRTVANILAYDEKNQLASY
LEKPFYDKKLAIIGITLDQMKNIARPDPDTHFVDAIATTARTVAHLHAKGIKHIIVLSHLGLDQDKRLAEQVDGISLIVG
GHTHTLQGDFSALGLSNIPYGETIHGTPIVHAGKYAETLGLAEIEFDAAGRAISLKGGNYFMLDKQFMLSSSEQVDDTDY
RAVQQRFNEHPYVLWDDADEDIHGVIQDIYRPAIAEMENKVLALVPKNLVHTRLPSKALPHGSEIAPWVSRSMFQEAKLE
DPRIHFALHNAGGVRQSVAQGRLTLADVLGQLLPFELPLVKYEIQGQYLYQALESAINAATNNSVRGTGAGSFPYTYGLR
YYYDGRRPLGERLFKLEMMQQDGLSLWYPVQPNALYVGVSTSYTAAGKEGYHPLLQARWQESIETLTLPQAFIRFLNRAP
NPNLADLLSPHVHYTSHVKRSV

Specific function: Degradation Of External Udp-Glucose To Uridine Monophosphate And Glucose-1-Phosphate, Which Can Then Be Used By The Cell. [C]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=552, Percent_Identity=25.7246376811594, Blast_Score=131, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28573524, Length=573, Percent_Identity=28.4467713787086, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI19922444, Length=555, Percent_Identity=26.4864864864865, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24641187, Length=550, Percent_Identity=26, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922446, Length=513, Percent_Identity=25.1461988304094, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24654424, Length=513, Percent_Identity=25.1461988304094, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221329836, Length=546, Percent_Identity=25.4578754578755, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843
- InterPro:   IPR006420 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 64842; Mature: 64711

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGYTLSLAHINDTHSNFEPSRVQFSFNLGLKALDITSHSGGYARLGYQISQARDRAAQ
CCCCEEEEEEEECCCCCCCCCCEEEEEEECCEEEEEEECCCCCEEEECEEHHHHHHHHHH
SQMPFLFLHGGDSFQGTLYFSHFKGKANAHLLNLLSPDAMVIGNHDIDEGNARLAEFAKQ
HCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IDFPLLAGNMDLSQEDIRKPGNLRTVANILAYDEKNQLASYLEKPFYDKKLAIIGITLDQ
CCCCEEECCCCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEEHHH
MKNIARPDPDTHFVDAIATTARTVAHLHAKGIKHIIVLSHLGLDQDKRLAEQVDGISLIV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCEEEEE
GGHTHTLQGDFSALGLSNIPYGETIHGTPIVHAGKYAETLGLAEIEFDAAGRAISLKGGN
CCCEEEECCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHCCEEEEECCCCCEEEECCCC
YFMLDKQFMLSSSEQVDDTDYRAVQQRFNEHPYVLWDDADEDIHGVIQDIYRPAIAEMEN
EEEEEHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
KVLALVPKNLVHTRLPSKALPHGSEIAPWVSRSMFQEAKLEDPRIHFALHNAGGVRQSVA
CEEEEECHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHH
QGRLTLADVLGQLLPFELPLVKYEIQGQYLYQALESAINAATNNSVRGTGAGSFPYTYGL
HCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECE
RYYYDGRRPLGERLFKLEMMQQDGLSLWYPVQPNALYVGVSTSYTAAGKEGYHPLLQARW
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
QESIETLTLPQAFIRFLNRAPNPNLADLLSPHVHYTSHVKRSV
HHHHHHEEHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TQGYTLSLAHINDTHSNFEPSRVQFSFNLGLKALDITSHSGGYARLGYQISQARDRAAQ
CCCEEEEEEEECCCCCCCCCCEEEEEEECCEEEEEEECCCCCEEEECEEHHHHHHHHHH
SQMPFLFLHGGDSFQGTLYFSHFKGKANAHLLNLLSPDAMVIGNHDIDEGNARLAEFAKQ
HCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IDFPLLAGNMDLSQEDIRKPGNLRTVANILAYDEKNQLASYLEKPFYDKKLAIIGITLDQ
CCCCEEECCCCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEEHHH
MKNIARPDPDTHFVDAIATTARTVAHLHAKGIKHIIVLSHLGLDQDKRLAEQVDGISLIV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCEEEEE
GGHTHTLQGDFSALGLSNIPYGETIHGTPIVHAGKYAETLGLAEIEFDAAGRAISLKGGN
CCCEEEECCCHHHCCCCCCCCCCCCCCCCCEECCCHHHHHCCEEEEECCCCCEEEECCCC
YFMLDKQFMLSSSEQVDDTDYRAVQQRFNEHPYVLWDDADEDIHGVIQDIYRPAIAEMEN
EEEEEHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
KVLALVPKNLVHTRLPSKALPHGSEIAPWVSRSMFQEAKLEDPRIHFALHNAGGVRQSVA
CEEEEECHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHH
QGRLTLADVLGQLLPFELPLVKYEIQGQYLYQALESAINAATNNSVRGTGAGSFPYTYGL
HCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECE
RYYYDGRRPLGERLFKLEMMQQDGLSLWYPVQPNALYVGVSTSYTAAGKEGYHPLLQARW
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
QESIETLTLPQAFIRFLNRAPNPNLADLLSPHVHYTSHVKRSV
HHHHHHEEHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]