Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 117919484

GI number: 117919484

Start: 1209065

End: 1211164

Strand: Direct

Name: pnp [H]

Synonym: Shewana3_1035

Alternate gene names: 117919484

Gene position: 1209065-1211164 (Clockwise)

Preceding gene: 117919482

Following gene: 117919485

Centisome position: 24.32

GC content: 47.62

Gene sequence:

>2100_bases
GTGAATCCAATTGTAAAGAGTTTTGAGTATGGTCAACATACAGTCACCCTGGAAACAGGTGTTATTGCACGTCAAGCAGA
CGCTGCCGTTTTAGCTAGCATGGGTGACACGACAGTGTTAGTCACTGTTGTTGGTAAAAAAGAAGCTGAAGCAGGCCGTG
ACTTCTTTCCTCTGACTGTTAACTATCAAGAAAAGACCTACGCAGCAGGTAAAATTCCTGGTGGTTTCTTTAAGCGTGAA
GGCCGTCCTTCAGAAGATGAGACATTAATCGCTCGCCTGATCGACCGTCCAATTCGTCCTCTTTTCCCTAATGGCTTCAC
AAACGAAGTTCAAGTGATCATTACTGTTGTATCTGTCGATCCACAAATCGAGCCGGATATTATTTCAATGATCGGTACTT
CGGCTGCGCTTGCTATTTCGGGTATCCCATTCAGCGGCCCATTAGGTGCAGCGCGCGTGGGTTACATCAATGGCGAATAC
GTACTGAACCCAACAGTGACTCAGTTAGAAAGCAGCCAACTGAACCTAGTTGTTGCCGGTACTGAGAGCGCGGTATTGAT
GGTTGAGTCAGAAGCTCAAGCATTACCAGAAGAAGTGATGTTAGGCTCAGTGGTTTATGGCCATGACCAACAACAAGTGG
TTATCAAAGCGATTGCTGAATTCAAAGCAGAAGCGGGTAAACCAGCTTGGAATTGGACTGCACCAGTTGCCAATGAAGCC
TTAGTTGCTCAAGTTAAAGAATTAGCTGAAGACGGTTTAGCGCAAGCGTATCAAATTCAAGTTAAACAAGATCGTTATGC
TCAAGTTGCTGTGGTTAAAGCCGCTGCTAAAGAAGCACTGTTGGCTGCAAACCCAGACGTCGATTTACGTGAAGTTGACG
GTTTGCTCGGTAGCTTAGAGAAGAAAGTGGTTCGTGGTCGCATCATCCGTGGTGAGCCTCGTATCGACGGTCGTGAGCCA
GACATGGTTCGTGCTCTCAGCGTATTAGCAGGCGTATTACCACGTACTCACGGTAGTGCACTGTTTACCCGTGGCGAAAC
TCAAGCACTGGTAACTTGTACTCTAGGTACTGAGCGTGACGCACAGAAGATTGATAGCATCATGGGCGAGCGTACTAACC
GCTTTATGCTGCACTACAACTTCCCTCCTTACTCTGTGGGTGAGACAGGTATGGTGGGTTCACCTAAGCGTCGCGAAATC
GGTCACGGCAAGTTAGCATGGCGCGGTATCAACGCAGTCATGCCAACGGCTGAAGAATTCCCATACAGCGTTCGCGTGGT
TTCTGAAATCACTGAATCTAACGGTTCAAGCTCTATGGCATCAGTTTGTGGTACGTCTCTTGCGCTGATGGATGCGGGTG
TACCAATCAAGACTTCTGTAGCGGGTATCGCTATGGGTCTAGTAAAAGAAGGCGACAACTTCGTTGTTCTGTCTGACATC
TTAGGTGATGAAGACCACTTAGGTGACATGGACTTCAAAGTAGCCGGTACTCGTGACGGTGTGACTGCACTGCAGATGGA
CATCAAGATCGAAGGTATCACCAAAGAAATCATGGAAATTGCACTGCAACAAGCCTACGGCGCACGTGTACATATCCTGA
ACGTGATGGACCAAGCGATTGGTTCACACCGTGATGATATCTCTGACCACGCTCCACGTATCACAACTATCAAGATCAAC
CCAGAGAAGATCCGTGATGTTATCGGTAAAGGCGGCGCGGTTATTCGTGCGCTGACCGAAGAAACCGGCACCACTATCGA
ACTGGAAGATGACGGTACTGTGAAGATTGCTTCGTCAAATGGCGATGCAACCCGTGAAGCCATCCGTCGTATCGAAGAAA
TCACTTCAGAAGTTGAAGTGGGTCGTATCTACAACGGTAAAGTTATCCGTATCGTAGACTTCGGTGCCTTCGTTAACATT
CTGCCTGGCAAAGATGGTTTAGTACACATTTCGCAAATCAGCGATGAGCGTGTTGCTAACGTCTCTGACCACCTTGAACT
GAACCAAGAAGTTACCGTTAAGGTAATGGAAGTGGATCGTCAAGGTCGTGTAAGACTGTCAATCAAAGAAGCACAGACTA
AAGAAGCCGCTGCTGAATAA

Upstream 100 bases:

>100_bases
AGATAGACCTTTGTCATCATTATCTGCTCTTTATCTATTGTGGATAATTGCAGTATACTTACGCGCGTAATTCAAGGTCA
TTAATTAAGGAATGGGTCAC

Downstream 100 bases:

>100_bases
TCGTCTTTACTGACATTATCGGCGTCAGTTTCGACATGACGTGACTAAAAGGAGATCAATTGATCTCCTTTTTGTTTGTT
TAGGTTTAATAAAATTGCGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVTQLESSQLNLVVAGTESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEDGLAQAYQIQVKQDRYAQVAVVKAAAKEALLAANPDVDLREVDGLLGSLEKKVVRGRIIRGEPRIDGREP
DMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPTAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGDATREAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE

Sequences:

>Translated_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVTQLESSQLNLVVAGTESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEDGLAQAYQIQVKQDRYAQVAVVKAAAKEALLAANPDVDLREVDGLLGSLEKKVVRGRIIRGEPRIDGREP
DMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPTAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGDATREAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE
>Mature_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVTQLESSQLNLVVAGTESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
LVAQVKELAEDGLAQAYQIQVKQDRYAQVAVVKAAAKEALLAANPDVDLREVDGLLGSLEKKVVRGRIIRGEPRIDGREP
DMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPTAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGDATREAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=719, Percent_Identity=37.6912378303199, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=73.5166425470333, Blast_Score=1048, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=681, Percent_Identity=32.8928046989721, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=36.8495077355837, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=36.8495077355837, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=36.8495077355837, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.5296803652968, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75366; Mature: 75366

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVTQLESSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHCCCCCEEEEEEC
TESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
CCCEEEEEECHHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQVKELAEDGLAQAYQIQVKQDRYAQVAVVKAAAKEALLAANPDVDLREVDGLLGSLE
HHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GDATREAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CCHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE
CHHHHCCCCCEEEEEEEECCCCCEEEEEEHHHCHHHCCC
>Mature Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFTNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVTQLESSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHCCCCCEEEEEEC
TESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPAWNWTAPVANEA
CCCEEEEEECHHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQVKELAEDGLAQAYQIQVKQDRYAQVAVVKAAAKEALLAANPDVDLREVDGLLGSLE
HHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GDATREAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CCHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEAAAE
CHHHHCCCCCEEEEEEEECCCCCEEEEEEHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA