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The map label for this gene is ubiF [H]

Identifier: 117919459

GI number: 117919459

Start: 1180756

End: 1181982

Strand: Direct

Name: ubiF [H]

Synonym: Shewana3_1010

Alternate gene names: 117919459

Gene position: 1180756-1181982 (Clockwise)

Preceding gene: 117919451

Following gene: 117919460

Centisome position: 23.75

GC content: 51.75

Gene sequence:

>1227_bases
ATGAGCCAAGCGATAAGCCAAACACAGCAACCAAGCCAGACAACCACAGCTACTCAGCTATTTGATGTGATCCTTGTCGG
TGGCGGTATGGTGGGCGCGGCAACGGCTATCGGGCTGGCGAAGCAAGGCTTACAGGTGGCGGTGATTGAATCATTTGCCC
CCGAGGCTTATTCCCCCGAGCAGGCATTAGATGTGCGCGTGTCGGCGATAAGTGTGGCCTCCGAACAGTTACTCGAACAA
CTCGGCGCGCTCGATAGTCTGCTTAACATGCGTAATGTGCCTTATCTGGGCTTAGAAACCTGGGAGCTTGATGGTTGCAT
TACCCAATTCCATAGCTCACAAATTGGCGCGAGCCATTTAGGGCATATTGTTGAAAACCGCTTGGTGCAACTCGCCCTGT
GGGAGCAAATGCAGCAATGGGATGCGATTAAATTGTTTTGCCCCGAGCGTGTGGCGACATTTTCGCGCCTCGCAGACGGG
GTGAGTGTGCACTTACAATCCGGTATCAAGCTTGAGGCAAAGCTGCTCATCGGGGCCGATGGCGCCAATTCGCAGGTGCG
CCAATGGGCGGGGATTGGCATTTCGGGGTGGGACTATGCTCAATCGGCGATGCTGATTAATATCCAAACCGCCCAAGGCC
AGCAAGATGTTACTTGGCAACAATTTACCCCTAACGGCCCGCGCTCGTTATTACCTCTACCCGGCAATCATGCGTCGTTA
GTCTGGTATGACGATGCCAATCGCATTAAACAGTTAATGCAGCTGAATCATAAGCAGCTTGCCGACCAAATCCGCCAGCA
TTTTCCCGCTCGCTTGGACAGTGACTTTACCGTTGAAGCTAAGGGCAGCTTTGCCCTCACGCGCCGCCACGCGAATGCCT
ATTATAAGCCGAATGTGGTTATTTTAGGGGATGCTGCGCACACCATTAACCCGTTGGCGGGCCAAGGGGTTAACCTCGGC
TTTAAGGATGTCGAAGCCTTACTGGCGGTCATCAAGACTGCATTAACCGAGGATAAACCTTGGTGGTCCACCGAGGTATT
AAATGCCTATCAAGCTAAACGTTATCGCGATAATCAGCTGATGATGACGACCATGGATGTGTTTTACGCAGGCTTTAGTA
ATGACATTCTGCCACTTAAATTACTGCGTAACGGCGCGTTAAAACTGGCCAATATTAATTCTCCCATCAAGCGAACCGTG
CTGAAGTATGCGATGGGGCTCAATTAG

Upstream 100 bases:

>100_bases
TCAGCGTGATGCGCTTTAAGCTTGGGGCTTAAGTTCGAGCTCTATCTAAGAGTAAAGTGGGTCAGATAAATGGAAAGAAT
TCTAAAGAGTGGTAACTAAG

Downstream 100 bases:

>100_bases
GCCACATCGTGAGTGCTGACTCACTCGGCATTTGTTGACTTACGCATTGAGTGCTAAAATGCCGCGTTTTTAACGAGCAT
AAGCGGCATTATTAACTCAA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSQAISQTQQPSQTTTATQLFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPEQALDVRVSAISVASEQLLEQ
LGALDSLLNMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLADG
VSVHLQSGIKLEAKLLIGADGANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYYKPNVVILGDAAHTINPLAGQGVNLG
FKDVEALLAVIKTALTEDKPWWSTEVLNAYQAKRYRDNQLMMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTV
LKYAMGLN

Sequences:

>Translated_408_residues
MSQAISQTQQPSQTTTATQLFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPEQALDVRVSAISVASEQLLEQ
LGALDSLLNMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLADG
VSVHLQSGIKLEAKLLIGADGANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYYKPNVVILGDAAHTINPLAGQGVNLG
FKDVEALLAVIKTALTEDKPWWSTEVLNAYQAKRYRDNQLMMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTV
LKYAMGLN
>Mature_407_residues
SQAISQTQQPSQTTTATQLFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPEQALDVRVSAISVASEQLLEQL
GALDSLLNMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLADGV
SVHLQSGIKLEAKLLIGADGANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASLV
WYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYYKPNVVILGDAAHTINPLAGQGVNLGF
KDVEALLAVIKTALTEDKPWWSTEVLNAYQAKRYRDNQLMMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTVL
KYAMGLN

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=385, Percent_Identity=31.6883116883117, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI32967307, Length=328, Percent_Identity=31.7073170731707, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1786883, Length=390, Percent_Identity=48.4615384615385, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=37.9134860050891, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789274, Length=402, Percent_Identity=27.363184079602, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25146631, Length=438, Percent_Identity=27.8538812785388, Blast_Score=158, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321694, Length=456, Percent_Identity=25.219298245614, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19920760, Length=439, Percent_Identity=28.9293849658314, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 44848; Mature: 44717

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAISQTQQPSQTTTATQLFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPE
CCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCC
QALDVRVSAISVASEQLLEQLGALDSLLNMRNVPYLGLETWELDGCITQFHSSQIGASHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEHHHHHHHHHHCCCCHHHH
GHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLADGVSVHLQSGIKLEAKLLIGAD
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCEEEECCCCEEEEEEEEECC
GANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
CCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEE
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYYKPNVV
EEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCEECCCEE
ILGDAAHTINPLAGQGVNLGFKDVEALLAVIKTALTEDKPWWSTEVLNAYQAKRYRDNQL
EECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEE
MMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTVLKYAMGLN
EEEEHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQAISQTQQPSQTTTATQLFDVILVGGGMVGAATAIGLAKQGLQVAVIESFAPEAYSPE
CCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCC
QALDVRVSAISVASEQLLEQLGALDSLLNMRNVPYLGLETWELDGCITQFHSSQIGASHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEHHHHHHHHHHCCCCHHHH
GHIVENRLVQLALWEQMQQWDAIKLFCPERVATFSRLADGVSVHLQSGIKLEAKLLIGAD
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCEEEECCCCEEEEEEEEECC
GANSQVRQWAGIGISGWDYAQSAMLINIQTAQGQQDVTWQQFTPNGPRSLLPLPGNHASL
CCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEE
VWYDDANRIKQLMQLNHKQLADQIRQHFPARLDSDFTVEAKGSFALTRRHANAYYKPNVV
EEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCEECCCEE
ILGDAAHTINPLAGQGVNLGFKDVEALLAVIKTALTEDKPWWSTEVLNAYQAKRYRDNQL
EECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEE
MMTTMDVFYAGFSNDILPLKLLRNGALKLANINSPIKRTVLKYAMGLN
EEEEHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]