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The map label for this gene is rluF [H]

Identifier: 117919365

GI number: 117919365

Start: 1071423

End: 1072133

Strand: Reverse

Name: rluF [H]

Synonym: Shewana3_0916

Alternate gene names: 117919365

Gene position: 1072133-1071423 (Counterclockwise)

Preceding gene: 117919366

Following gene: 117919363

Centisome position: 21.56

GC content: 50.35

Gene sequence:

>711_bases
ATGCGTCTCGCCAAATACCTTGCCCAATGCGGCATTAGTTCACGCCGTGAAGCCTGCCGCTTGATTGAGGCCGGACGCAT
CAGCCTCAATGGCAAGATAGCCAAGCATACCGATGCCGTGCATTTCGATGCTGACGGCCAATGTCTCGATAGCCTCTGCT
TAGATGAAAAGCCCATTTCGGGCGCCGAAGCACTCAGCTATTGGATCTTCAATAAAGCCGTGGGCACAGATTGCCGTTTG
TTAGACGCCGATAAATCCAGTTTGCTGCATTTACTGCCAACTACGCCAAGACTCTACCCTGTTGGCAGATTAGACAAGGA
TTCCCGCGGTTTATTATTGCTGACTAACGATGGCGAACTCACCCATAAACTGATGCACCCTAGTTTTGCCCACAGTAAAA
CCTACCATGTGCAGCTCGATAGACCCTTCAACGATGCCTTTATCGCGCAAATGGCCAGCGGCGTGCAATACAAAGAAGTA
CGCACACTCCCCTGCCAAGTCACACGGTTAAGCGCAGACCGTTTTGAAATTGTGCTCACCCAAGGGTTAAATCGCCAAAT
TCGGCGCATGTCCAACGCCTTGGGTTATAAGGTGATTGACTTACAACGCGTCGCGTTAATGACATTAACCCTAGCATCCT
TAGCCGAAGGCGAAATGCGCCCACTCAGCCCAGAGGAAATTACAACACTTAAAGCCGCAGTGATGATTTAG

Upstream 100 bases:

>100_bases
ATATTATTTGGTGCTTAAGCCTCGCATTTGCCATGCGCCGTTTATCCAAAATCCTGTATTATCGCGCCCAAGTCCATCAC
CCGTTTTAGCTTAGTCATTT

Downstream 100 bases:

>100_bases
TGAGGACAAGTAGCGAATGCCCTCAAGCAGCTAACCTCAATATAAGACAAATGCCTGCGCGAACAATATCTTCCGATGAA
CGCCAATACCAGTAAAAACG

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRLAKYLAQCGISSRREACRLIEAGRISLNGKIAKHTDAVHFDADGQCLDSLCLDEKPISGAEALSYWIFNKAVGTDCRL
LDADKSSLLHLLPTTPRLYPVGRLDKDSRGLLLLTNDGELTHKLMHPSFAHSKTYHVQLDRPFNDAFIAQMASGVQYKEV
RTLPCQVTRLSADRFEIVLTQGLNRQIRRMSNALGYKVIDLQRVALMTLTLASLAEGEMRPLSPEEITTLKAAVMI

Sequences:

>Translated_236_residues
MRLAKYLAQCGISSRREACRLIEAGRISLNGKIAKHTDAVHFDADGQCLDSLCLDEKPISGAEALSYWIFNKAVGTDCRL
LDADKSSLLHLLPTTPRLYPVGRLDKDSRGLLLLTNDGELTHKLMHPSFAHSKTYHVQLDRPFNDAFIAQMASGVQYKEV
RTLPCQVTRLSADRFEIVLTQGLNRQIRRMSNALGYKVIDLQRVALMTLTLASLAEGEMRPLSPEEITTLKAAVMI
>Mature_236_residues
MRLAKYLAQCGISSRREACRLIEAGRISLNGKIAKHTDAVHFDADGQCLDSLCLDEKPISGAEALSYWIFNKAVGTDCRL
LDADKSSLLHLLPTTPRLYPVGRLDKDSRGLLLLTNDGELTHKLMHPSFAHSKTYHVQLDRPFNDAFIAQMASGVQYKEV
RTLPCQVTRLSADRFEIVLTQGLNRQIRRMSNALGYKVIDLQRVALMTLTLASLAEGEMRPLSPEEITTLKAAVMI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=38.034188034188, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=32.9113924050633, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787524, Length=223, Percent_Identity=33.6322869955157, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26295; Mature: 26295

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAKYLAQCGISSRREACRLIEAGRISLNGKIAKHTDAVHFDADGQCLDSLCLDEKPIS
CCHHHHHHHCCCCHHHHHHHHHHCCEEEECCEEECCCCEEEECCCCHHHHHHHCCCCCCC
GAEALSYWIFNKAVGTDCRLLDADKSSLLHLLPTTPRLYPVGRLDKDSRGLLLLTNDGEL
HHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCEEECCCCCCCCCCEEEEECCCHH
THKLMHPSFAHSKTYHVQLDRPFNDAFIAQMASGVQYKEVRTLPCQVTRLSADRFEIVLT
HHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHCCCCEEEEEECCCCEEEEEH
QGLNRQIRRMSNALGYKVIDLQRVALMTLTLASLAEGEMRPLSPEEITTLKAAVMI
HCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECC
>Mature Secondary Structure
MRLAKYLAQCGISSRREACRLIEAGRISLNGKIAKHTDAVHFDADGQCLDSLCLDEKPIS
CCHHHHHHHCCCCHHHHHHHHHHCCEEEECCEEECCCCEEEECCCCHHHHHHHCCCCCCC
GAEALSYWIFNKAVGTDCRLLDADKSSLLHLLPTTPRLYPVGRLDKDSRGLLLLTNDGEL
HHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCEEECCCCCCCCCCEEEEECCCHH
THKLMHPSFAHSKTYHVQLDRPFNDAFIAQMASGVQYKEVRTLPCQVTRLSADRFEIVLT
HHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHCCCCEEEEEECCCCEEEEEH
QGLNRQIRRMSNALGYKVIDLQRVALMTLTLASLAEGEMRPLSPEEITTLKAAVMI
HCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]