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The map label for this gene is htrB [H]

Identifier: 117919300

GI number: 117919300

Start: 985378

End: 986229

Strand: Reverse

Name: htrB [H]

Synonym: Shewana3_0848

Alternate gene names: 117919300

Gene position: 986229-985378 (Counterclockwise)

Preceding gene: 117919304

Following gene: 117919298

Centisome position: 19.83

GC content: 50.35

Gene sequence:

>852_bases
ATGCGGATTACCCAAGTGTTACCTCTTTCGTGGCAGATGAAGTTAGGTAAAGGATTAGGCCGATTAGTTAAGACTATTGC
AGGCAGCCGTACCCATACCGCGCGCCGCAATTTAACTCTCTGCTTCCCCGACATGCCCGAGTCAGAGCGCGAAGCCCTGC
TGACACGTAACTTTGAAGAAACGGGTAAAGCGATTTTTGATACCATCAATGCCTGGTGGTGGACCGATGAAAAAATCCAA
CAGCATATGCAGATCACCGGCAAAGAATACGTGCAAGACACCTTGAATGCCGGCCATGGGGTGATTCTGTTTGCCGTGCA
CTGCTTACCACTGGAAATGGGCGCGCGTATTTTTGGTCAATTCCAACCCGGTGTTGGGGTGTATCGTCCACACAACAATC
CGGTGATGGAATATCTACAGGTAAAAGGCCGCTTACGCTCAAACAAAGCCTTAGTGCCGAAACGCGATCTACGCCAGATG
GTGCGTTGTTTACGCAATCCCGATGTGATTTGGTATACCGCCGATCAAGATTTTGGGCGTTCGAGTGCGGTGTTTATTCC
CTTCTATGCCGTGCCCGATGCCGCGACCATTACCGGTGCCACCACACTGGCTAAACTCGGTAAGGCCAAAGTGCTGCCCT
TCTTTGTGGAGCGCACCGAAGGCGATAAGGGTTACCATATTGAGATTATGCCGCCGCTGGATAACTTCCCCGGTGAAGAT
GAGCTAGCCGATGCCATTCGTGGTAACAAAATTATCGAAGGGATTATCGATAGAAATCGCGCCCAATATATGTGGCTTCA
CCGCCGCTTTAAGACTCGCCCAGATCCCACGGACAAGTCCTTATATAAATAA

Upstream 100 bases:

>100_bases
ATTTTCAAAAGTAGTGAGTATTTTTGTGGTCGAGAAAGCTGAGTTTTCATCATCCTTATATCATCCGAAGCACTGGCCCA
TGTGGTTTGGGGTGTTAGTG

Downstream 100 bases:

>100_bases
GCTTTCGTTCTATCTGTTCAAGATAAGTGCTTAAAAGCGGATAAAAAAATGCCAAGCAATTGCTTGGCATTTTTTGTTGG
ATGTTTTGTTTAAGGCATTT

Product: lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRITQVLPLSWQMKLGKGLGRLVKTIAGSRTHTARRNLTLCFPDMPESEREALLTRNFEETGKAIFDTINAWWWTDEKIQ
QHMQITGKEYVQDTLNAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKRDLRQM
VRCLRNPDVIWYTADQDFGRSSAVFIPFYAVPDAATITGATTLAKLGKAKVLPFFVERTEGDKGYHIEIMPPLDNFPGED
ELADAIRGNKIIEGIIDRNRAQYMWLHRRFKTRPDPTDKSLYK

Sequences:

>Translated_283_residues
MRITQVLPLSWQMKLGKGLGRLVKTIAGSRTHTARRNLTLCFPDMPESEREALLTRNFEETGKAIFDTINAWWWTDEKIQ
QHMQITGKEYVQDTLNAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKRDLRQM
VRCLRNPDVIWYTADQDFGRSSAVFIPFYAVPDAATITGATTLAKLGKAKVLPFFVERTEGDKGYHIEIMPPLDNFPGED
ELADAIRGNKIIEGIIDRNRAQYMWLHRRFKTRPDPTDKSLYK
>Mature_283_residues
MRITQVLPLSWQMKLGKGLGRLVKTIAGSRTHTARRNLTLCFPDMPESEREALLTRNFEETGKAIFDTINAWWWTDEKIQ
QHMQITGKEYVQDTLNAGHGVILFAVHCLPLEMGARIFGQFQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKRDLRQM
VRCLRNPDVIWYTADQDFGRSSAVFIPFYAVPDAATITGATTLAKLGKAKVLPFFVERTEGDKGYHIEIMPPLDNFPGED
ELADAIRGNKIIEGIIDRNRAQYMWLHRRFKTRPDPTDKSLYK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=269, Percent_Identity=42.3791821561338, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI87082094, Length=268, Percent_Identity=38.4328358208955, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788161, Length=267, Percent_Identity=27.3408239700375, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 32400; Mature: 32400

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITQVLPLSWQMKLGKGLGRLVKTIAGSRTHTARRNLTLCFPDMPESEREALLTRNFEE
CCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEECCCCCHHHHHHHHCCHHH
TGKAIFDTINAWWWTDEKIQQHMQITGKEYVQDTLNAGHGVILFAVHCLPLEMGARIFGQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHC
FQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKRDLRQMVRCLRNPDVIWYTADQDFGR
CCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
SSAVFIPFYAVPDAATITGATTLAKLGKAKVLPFFVERTEGDKGYHIEIMPPLDNFPGED
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCHH
ELADAIRGNKIIEGIIDRNRAQYMWLHRRFKTRPDPTDKSLYK
HHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRITQVLPLSWQMKLGKGLGRLVKTIAGSRTHTARRNLTLCFPDMPESEREALLTRNFEE
CCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEECCCCCHHHHHHHHCCHHH
TGKAIFDTINAWWWTDEKIQQHMQITGKEYVQDTLNAGHGVILFAVHCLPLEMGARIFGQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHC
FQPGVGVYRPHNNPVMEYLQVKGRLRSNKALVPKRDLRQMVRCLRNPDVIWYTADQDFGR
CCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
SSAVFIPFYAVPDAATITGATTLAKLGKAKVLPFFVERTEGDKGYHIEIMPPLDNFPGED
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCHH
ELADAIRGNKIIEGIIDRNRAQYMWLHRRFKTRPDPTDKSLYK
HHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 7592970 [H]