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The map label for this gene is speE [H]

Identifier: 117919281

GI number: 117919281

Start: 958991

End: 960724

Strand: Reverse

Name: speE [H]

Synonym: Shewana3_0829

Alternate gene names: 117919281

Gene position: 960724-958991 (Counterclockwise)

Preceding gene: 117919282

Following gene: 117919280

Centisome position: 19.32

GC content: 50.06

Gene sequence:

>1734_bases
ATGCACGAATCCCAAACAACCAGCTCGGCGACTCAAGCGGTACAAACGAGTCGCCTAAGCTGGTTCGATGATGTCCTACT
ATTGGGCATTATGGCCGTGCTCGCTGGGTGCGGCCTGATCTACGAATACCTGTTGTCCCACTACGCAGGCCGGATCTTAG
GCGCGTTAGAAGCCGCCATTTACACTATGATCGGCCTGATGATCGTCTCCATGGGTCTTGGCGCCTTTGCCGCACGCAAA
ATTAAAGATGCCTTTACTGGCTTTGTGGTGCTGGAACTCACGGTCGCGCTCTGTGGCTCGCTGGCGATTTTAATTACCGC
TGCCGTGATAGGTTTTGGTCAGCAGCTGCCGATGCTTATCGCCTCCACCTTAGGTTTACCACCGGATCAACTGCCCGAAG
GTGGCATGATTGGCTCGCTGCAAAAGCTGAGCGAACATCTGCCCTATGTCTGGGGCGTGCTGCTCGGCTTGATGATCGGG
ATGGAAATTCCGCTGATCGCGAGGGTGAGACAATCCCTATCAGATGAACACTTACTGCATAATGCCGGTACCATTTATGG
CGCCGACTATATTGGTGCAGGCATTGGCGCGGCCATTTGGGTCGGCTTTATGCTGGCCATCGATATTCAACTCGCCGCAG
CTCTGACCGCCAGCTTTAACTTGCTCGCCGGCTTTGTGTTTATCTGGCGTTTTTGGCCCAAAATCCAAAGAGCAAAATTA
TTGCTCGCGGGGCATTTTGTGGTCACGGGCGTATTATTATTGCTCGCCATTCAAGGGCCGAGTTGGGAGCAACAGTTCAA
CAATCTACTTTATAAAGACAAAGTGGTTTACGCTAAGGCCACGCGTTTTCAACAACTTACCTTTACCGAGCGCTTACGCG
GCAATGGCCTCTCCCCCATATACTCTTTGTATATCAATGGCCGATTACAGTTTTCGAGTATCGATGAGCATATCTACCAT
GCGTTTTTAGTTCACCCAACGCTTGCGGCCAGCGCTCGCCACAACAAGGTATTAATCATTGGTGGTGGTGATGGACTGGG
CCTAAAGCAAGTGCTGCGTTGGGAACCTGAGCAAGTGACCTTGCTCGATCTTGATGCCGCATTGGTGCAACTCTTTAAAA
CGCCCGATACGGATATGCCTAAACGCCTAAGCCAAGCCCTGTTAGCCCTTAATGGCAATGCCTTTAACGATCCTCGCGTT
AAGGTGATTAACGACGATGCCTTTAATGGAGTCGATAAATTGATTGCCGCAGGAGATAAATACGACGCCATTATTGTGGA
CTTGCCCGATCCTAGCCATCCCGATTTAAATAAGCTCTATTCGGATTATTTCTATCGCAAGCTCAAGGAACTAATGAGCA
ATGATGGCGCTCTGACTGTACAGTCAACCTCACCCTATCACGCGCAAAAGGCCTTTATCTCGGTAGCCAAAACCTTGGCC
TTAGCGGGGTTTGACGTAAAACAGTATCACCATAACGTCCCAAGCTTTGGAGAATGGGGCTGGAGTATTGCCACCCTATC
TGGAAAGGATGCCCAGCATCGCCTTGGCGAGCTGACCACACTACCGATAGCGGATGACTGGTTAACCTTAGGTTTAGTCA
AAGGTGCCTTTGAATTTCCTGCCAATTTTTATCAGGACGCCGCCAGTATTAAGCCTAACGAACTCGGTTCGTTACAGTTG
TACCATTATCATCAACAGGCATGGTCCGAAACCCAAGGACTGGATCTTTTTTAA

Upstream 100 bases:

>100_bases
GATGAAGTCGAAAGACAGCACAACATTGGTATCGCTGCCGTAGAAATGGCCATCAGTATTGCTATCGCACTTATTCTCAC
GGCCCTAATGGCGTAATCCA

Downstream 100 bases:

>100_bases
CGGATAAAGATAGTGCTTGACATATTATGTCTCGGTGCTATCTTTGAATACAGACATAATATGTCAATAAGGACAAATAT
GAATATCCATACTCTTGCTA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MHESQTTSSATQAVQTSRLSWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFAARK
IKDAFTGFVVLELTVALCGSLAILITAAVIGFGQQLPMLIASTLGLPPDQLPEGGMIGSLQKLSEHLPYVWGVLLGLMIG
MEIPLIARVRQSLSDEHLLHNAGTIYGADYIGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFVFIWRFWPKIQRAKL
LLAGHFVVTGVLLLLAIQGPSWEQQFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLSPIYSLYINGRLQFSSIDEHIYH
AFLVHPTLAASARHNKVLIIGGGDGLGLKQVLRWEPEQVTLLDLDAALVQLFKTPDTDMPKRLSQALLALNGNAFNDPRV
KVINDDAFNGVDKLIAAGDKYDAIIVDLPDPSHPDLNKLYSDYFYRKLKELMSNDGALTVQSTSPYHAQKAFISVAKTLA
LAGFDVKQYHHNVPSFGEWGWSIATLSGKDAQHRLGELTTLPIADDWLTLGLVKGAFEFPANFYQDAASIKPNELGSLQL
YHYHQQAWSETQGLDLF

Sequences:

>Translated_577_residues
MHESQTTSSATQAVQTSRLSWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFAARK
IKDAFTGFVVLELTVALCGSLAILITAAVIGFGQQLPMLIASTLGLPPDQLPEGGMIGSLQKLSEHLPYVWGVLLGLMIG
MEIPLIARVRQSLSDEHLLHNAGTIYGADYIGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFVFIWRFWPKIQRAKL
LLAGHFVVTGVLLLLAIQGPSWEQQFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLSPIYSLYINGRLQFSSIDEHIYH
AFLVHPTLAASARHNKVLIIGGGDGLGLKQVLRWEPEQVTLLDLDAALVQLFKTPDTDMPKRLSQALLALNGNAFNDPRV
KVINDDAFNGVDKLIAAGDKYDAIIVDLPDPSHPDLNKLYSDYFYRKLKELMSNDGALTVQSTSPYHAQKAFISVAKTLA
LAGFDVKQYHHNVPSFGEWGWSIATLSGKDAQHRLGELTTLPIADDWLTLGLVKGAFEFPANFYQDAASIKPNELGSLQL
YHYHQQAWSETQGLDLF
>Mature_577_residues
MHESQTTSSATQAVQTSRLSWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFAARK
IKDAFTGFVVLELTVALCGSLAILITAAVIGFGQQLPMLIASTLGLPPDQLPEGGMIGSLQKLSEHLPYVWGVLLGLMIG
MEIPLIARVRQSLSDEHLLHNAGTIYGADYIGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFVFIWRFWPKIQRAKL
LLAGHFVVTGVLLLLAIQGPSWEQQFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLSPIYSLYINGRLQFSSIDEHIYH
AFLVHPTLAASARHNKVLIIGGGDGLGLKQVLRWEPEQVTLLDLDAALVQLFKTPDTDMPKRLSQALLALNGNAFNDPRV
KVINDDAFNGVDKLIAAGDKYDAIIVDLPDPSHPDLNKLYSDYFYRKLKELMSNDGALTVQSTSPYHAQKAFISVAKTLA
LAGFDVKQYHHNVPSFGEWGWSIATLSGKDAQHRLGELTTLPIADDWLTLGLVKGAFEFPANFYQDAASIKPNELGSLQL
YHYHQQAWSETQGLDLF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786312, Length=299, Percent_Identity=28.0936454849498, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323175, Length=241, Percent_Identity=25.7261410788382, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325326, Length=194, Percent_Identity=27.319587628866, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 63233; Mature: 63233

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]