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The map label for this gene is dusA [H]

Identifier: 117919170

GI number: 117919170

Start: 836488

End: 837492

Strand: Direct

Name: dusA [H]

Synonym: Shewana3_0718

Alternate gene names: 117919170

Gene position: 836488-837492 (Clockwise)

Preceding gene: 117919169

Following gene: 117919171

Centisome position: 16.82

GC content: 49.55

Gene sequence:

>1005_bases
GTGTTGAAAAATAATCTTAATGATGGTCTACAGCTCGACCGCACCTTTTCCATTGCGCCCATGCTCGACTGGACGGATCG
TCATTACCGTTATTTTGCTCGCTTAATGTCGGCCAATGCCTTGCTGTATACCGAGATGGTGACGACGGGGGCGATTTTGC
ATGGCCGTGGTGATTATCTTGCCTATAACCAAGAAGAACATCCCCTTGCATTGCAATTGGGGGGCTCGAATCCTGCTGAG
CTTGCTCGCTGCGCCAAACTTGCCGCCGAGCGCGGTTATGATGAAGTGAACCTCAATGTGGGCTGTCCTTCCGATCGGGT
GCAAAACGGTCGCTTTGGTGCCTGTTTGATGGCGGAACCTGAGCTGGTGGCTGAATGTGTCGATGCCATGAAGCAAGCGG
TTGATATACCAGTGACGGTAAAAACACGTATCGGCATTGACGAGCAGGATAGCTATGAGTTTTTGACTCACTTTATTGAT
ACTGTTATGGCTAAAGGCTGTGGTGAATTTATTATCCATGCGCGTAAGGCGTGGTTACAGGGCTTAAGTCCGAAGGAAAA
TCGCGAGATCCCGCCCTTAGACTACGACAGGGTGTATCAGCTTAAGCGTGATTATCCGACGCTCAATATCAGCATTAACG
GTGGTATCACTAGCCTTGAACAGGCTAAAACTCACTTGCAACACTTAGATGGTGTGATGGTGGGACGTGAGGCTTACCAA
AATCCCTATATTCTGGCGCAGGTTGACCAACTGCTCTGTGGCAGTCAGAAGGCGGTGATGAGCCGTGAAGCTGTGATTGA
GACCATGTTGCCCTATATCGAGGCTCACTTGCAGGCGGGCGGGCGCTTAAATCATATCACTCGTCATATGATTGGCTTAT
TCCAAGGCTTACCGGGTGCCCGTGCGTGGCGTCGTTATTTGAGCGAAAACGCCCATAAAAATGGTGCAGGTATTGAGGTG
GTCAAGCAGGCTTACCAAAGTATTCAGAGTGAAATAACTGCTTAA

Upstream 100 bases:

>100_bases
CGCTGGCGCTGCATTGCTCATCAGCGGCTTAAATTAAGGCGCTTTATCCCTCTAAGCTTTAACTTACTGGCAAATTTTAA
TTACTTGAGTCGATAGTCAC

Downstream 100 bases:

>100_bases
TAATTATTCAATTTAAAAAGTGGTGAGATTGACTAATGCTTGTAGTATTTTTCACCACTTTACCTTTCCTAAATATCCCG
CTTATCTCGTTATAGTTTAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MLKNNLNDGLQLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPAE
LARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVDAMKQAVDIPVTVKTRIGIDEQDSYEFLTHFID
TVMAKGCGEFIIHARKAWLQGLSPKENREIPPLDYDRVYQLKRDYPTLNISINGGITSLEQAKTHLQHLDGVMVGREAYQ
NPYILAQVDQLLCGSQKAVMSREAVIETMLPYIEAHLQAGGRLNHITRHMIGLFQGLPGARAWRRYLSENAHKNGAGIEV
VKQAYQSIQSEITA

Sequences:

>Translated_334_residues
MLKNNLNDGLQLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPAE
LARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVDAMKQAVDIPVTVKTRIGIDEQDSYEFLTHFID
TVMAKGCGEFIIHARKAWLQGLSPKENREIPPLDYDRVYQLKRDYPTLNISINGGITSLEQAKTHLQHLDGVMVGREAYQ
NPYILAQVDQLLCGSQKAVMSREAVIETMLPYIEAHLQAGGRLNHITRHMIGLFQGLPGARAWRRYLSENAHKNGAGIEV
VKQAYQSIQSEITA
>Mature_334_residues
MLKNNLNDGLQLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGGSNPAE
LARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVDAMKQAVDIPVTVKTRIGIDEQDSYEFLTHFID
TVMAKGCGEFIIHARKAWLQGLSPKENREIPPLDYDRVYQLKRDYPTLNISINGGITSLEQAKTHLQHLDGVMVGREAYQ
NPYILAQVDQLLCGSQKAVMSREAVIETMLPYIEAHLQAGGRLNHITRHMIGLFQGLPGARAWRRYLSENAHKNGAGIEV
VKQAYQSIQSEITA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=302, Percent_Identity=25.4966887417219, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=25.5230125523013, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=24.7899159663866, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI145693211, Length=314, Percent_Identity=72.9299363057325, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1789660, Length=250, Percent_Identity=28, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788462, Length=178, Percent_Identity=32.5842696629214, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=170, Percent_Identity=27.6470588235294, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=24.4725738396624, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=26.8595041322314, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=26.8595041322314, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37362; Mature: 37362

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNNLNDGLQLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYL
CCCCCCCCCCEECCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEECCCCEE
AYNQEEHPLALQLGGSNPAELARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEP
EECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCEEEECCH
ELVAECVDAMKQAVDIPVTVKTRIGIDEQDSYEFLTHFIDTVMAKGCGEFIIHARKAWLQ
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
GLSPKENREIPPLDYDRVYQLKRDYPTLNISINGGITSLEQAKTHLQHLDGVMVGREAYQ
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
NPYILAQVDQLLCGSQKAVMSREAVIETMLPYIEAHLQAGGRLNHITRHMIGLFQGLPGA
CCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
RAWRRYLSENAHKNGAGIEVVKQAYQSIQSEITA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKNNLNDGLQLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYL
CCCCCCCCCCEECCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEECCCCEE
AYNQEEHPLALQLGGSNPAELARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEP
EECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCEEEECCH
ELVAECVDAMKQAVDIPVTVKTRIGIDEQDSYEFLTHFIDTVMAKGCGEFIIHARKAWLQ
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
GLSPKENREIPPLDYDRVYQLKRDYPTLNISINGGITSLEQAKTHLQHLDGVMVGREAYQ
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
NPYILAQVDQLLCGSQKAVMSREAVIETMLPYIEAHLQAGGRLNHITRHMIGLFQGLPGA
CCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
RAWRRYLSENAHKNGAGIEVVKQAYQSIQSEITA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]