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The map label for this gene is purA [H]

Identifier: 117919147

GI number: 117919147

Start: 810372

End: 811667

Strand: Direct

Name: purA [H]

Synonym: Shewana3_0695

Alternate gene names: 117919147

Gene position: 810372-811667 (Clockwise)

Preceding gene: 117919146

Following gene: 117919148

Centisome position: 16.3

GC content: 48.07

Gene sequence:

>1296_bases
ATGGGCAAAAACGTAGTTGTTCTCGGCACTCAATGGGGTGACGAAGGAAAAGGTAAGATTGTCGACCTTCTAACAGAACA
GGCAAAATATGTAGTTCGCTACCAAGGCGGTCACAACGCGGGTCACACTCTAGTGATCAATGGCGATAAAACCGTACTTC
ATCTGATCCCATCAGGCATCCTACGCGACAATGTGAAATGCATTATCGGTAACGGTGTGGTGCTTGCACCTGACGCCCTG
ATGAAAGAGATCAAAATGCTCAAAGAGCGCGGCATTCCTGTTGAGGAACGTCTGCTGATCTCTGAAGCATGTCCGCTGAT
CCTACCATTCCACTGTGCATTAGACATCGCTCGCGAAAAAGCGCGCGGTAACAAAGCTATTGGTACTACGGGTCGTGGTA
TTGGTCCTGCGTATGAAGACAAGATTTCCCGTCGCGGTCTGCGTGTTGGCGATCTGTTCAATGCTGAACTATTTGCTGAA
AAGCTGAAAGAAGTTATGGCATATCATAACTTTATGCTGACAGAGTACTACAAAGTTGACGCCGTAGATTACGAGCAAAC
ACTGAGCGACGCGTTAGCCTTAGCTGACTACCTGAAGAGCATGTGTGTTGACGTAACTGAACTGTTGGATAACGCGCGTA
AAGCGGGTGAGCCAATTCTGTTCGAAGGTGCACAAGGTACGCTGTTAGACATCGACCACGGTACTTATCCGTTTGTGACT
TCGTCAAACACTACCGCAGGTGGTGTGGCGACAGGCAGCGGCTTTGGTCCACGTCATTTAGACTATGTCTTAGGTATCAT
CAAGGCATACACCACTCGCGTGGGTGCTGGTCCTTTCCCAACTGAACTCTTATGTGAAGTGGGTGATCATTTAGGAACTA
AAGGTCATGAATTTGGTGCGACTACTGGTCGTAAGCGTCGTCCCGGTTGGTTAGATGCAGTGGCAATGCGCCGCGCAGTA
CAAATCAACAGTGTAAGCGGCTTCTGCTTAACTAAGTTAGACGTACTCGATGGCCTGAAAGAGGTGAAGATCTGTGTGGG
TTACCAACATCCAGATGGCACTATCTCTAAGGTTACGCCATTAGCGGCTGAAGGTTATGAGCAGGTCACGCCTGTTTATG
AAACTATGCCAGGCTGGAGCGAGTCAACTTTCGGTGCGACCTCTTTAGAGCAGTTACCGCAAGCAGCTATCAACTACATC
AAGCGTATTGAAGAGTTGTTAGAAACGCCGATCGACATCATTTCTACCGGTCCAGACCGTAATGAAACGATGATTTTAGT
TAGCCCATTTAACTAA

Upstream 100 bases:

>100_bases
CGCAGGGCTGGTCATTTTGATTATTTTTTCATAAATGCCTTACCACTTGCGGTTTAAAATCTGTTAGAATTCTTTTTTAA
CCACAACCTTGCAGCAAACA

Downstream 100 bases:

>100_bases
AATCGTAGAGGCCGTGTTCCACGGCCTTTTCTTCCTTTGTTGTGCCTTCCTGTTTGTTTTCTGTGGTTAGAAATTCGGCT
TGAGCTCAAGAAATAGGTTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVINGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MKEIKMLKERGIPVEERLLISEACPLILPFHCALDIAREKARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAE
KLKEVMAYHNFMLTEYYKVDAVDYEQTLSDALALADYLKSMCVDVTELLDNARKAGEPILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRHLDYVLGIIKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGATTGRKRRPGWLDAVAMRRAV
QINSVSGFCLTKLDVLDGLKEVKICVGYQHPDGTISKVTPLAAEGYEQVTPVYETMPGWSESTFGATSLEQLPQAAINYI
KRIEELLETPIDIISTGPDRNETMILVSPFN

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVINGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MKEIKMLKERGIPVEERLLISEACPLILPFHCALDIAREKARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAE
KLKEVMAYHNFMLTEYYKVDAVDYEQTLSDALALADYLKSMCVDVTELLDNARKAGEPILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRHLDYVLGIIKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGATTGRKRRPGWLDAVAMRRAV
QINSVSGFCLTKLDVLDGLKEVKICVGYQHPDGTISKVTPLAAEGYEQVTPVYETMPGWSESTFGATSLEQLPQAAINYI
KRIEELLETPIDIISTGPDRNETMILVSPFN
>Mature_430_residues
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVINGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDALM
KEIKMLKERGIPVEERLLISEACPLILPFHCALDIAREKARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAEK
LKEVMAYHNFMLTEYYKVDAVDYEQTLSDALALADYLKSMCVDVTELLDNARKAGEPILFEGAQGTLLDIDHGTYPFVTS
SNTTAGGVATGSGFGPRHLDYVLGIIKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGATTGRKRRPGWLDAVAMRRAVQ
INSVSGFCLTKLDVLDGLKEVKICVGYQHPDGTISKVTPLAAEGYEQVTPVYETMPGWSESTFGATSLEQLPQAAINYIK
RIEELLETPIDIISTGPDRNETMILVSPFN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=44.4964871194379, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=44.5221445221445, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=42.713567839196, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=76.7981438515081, Blast_Score=689, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=42.6267281105991, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=42.6267281105991, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=43.5897435897436, Blast_Score=333, Evalue=4e-92,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=42.9245283018868, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46879; Mature: 46748

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVINGDKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCIIGNGVVLAPDALMKEIKMLKERGIPVEERLLISEACPLILPFHCALDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAEKLKEVMAYHNFMLTEYYKVD
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
AVDYEQTLSDALALADYLKSMCVDVTELLDNARKAGEPILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPRHLDYVLGIIKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
TTGRKRRPGWLDAVAMRRAVQINSVSGFCLTKLDVLDGLKEVKICVGYQHPDGTISKVTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHEEEEEEEECCCCCHHHCCC
LAAEGYEQVTPVYETMPGWSESTFGATSLEQLPQAAINYIKRIEELLETPIDIISTGPDR
HHHCCHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
NETMILVSPFN
CCEEEEEECCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVINGDKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCIIGNGVVLAPDALMKEIKMLKERGIPVEERLLISEACPLILPFHCALDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAEKLKEVMAYHNFMLTEYYKVD
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
AVDYEQTLSDALALADYLKSMCVDVTELLDNARKAGEPILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPRHLDYVLGIIKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
TTGRKRRPGWLDAVAMRRAVQINSVSGFCLTKLDVLDGLKEVKICVGYQHPDGTISKVTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHEEEEEEEECCCCCHHHCCC
LAAEGYEQVTPVYETMPGWSESTFGATSLEQLPQAAINYIKRIEELLETPIDIISTGPDR
HHHCCHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
NETMILVSPFN
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA