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The map label for this gene is nrfA

Identifier: 117919110

GI number: 117919110

Start: 778296

End: 779717

Strand: Direct

Name: nrfA

Synonym: Shewana3_0658

Alternate gene names: 117919110

Gene position: 778296-779717 (Clockwise)

Preceding gene: 117919107

Following gene: 117919112

Centisome position: 15.65

GC content: 48.87

Gene sequence:

>1422_bases
ATGCAACACGGAGATGAGATGATGAAGAAGATGACAGGTAAGTCCTTTGCACTCAGTGCATTGGTTGCCGCGAGCTTCAT
GGCGGCGGGTGCGATGGCAAGCGATAAGACCGAGCCACGCAACGAAGTATACAAAGATAAGTTTGCTAATCAATACAATA
GCTGGCATGACACCGCAAAAAGTGAAGAGATCACCGACGCATTAGCGGGCGATCCTAGCTTAGTAATTCTGTGGGCCGGT
TATGGTTTCGCAAAAGACTACAACGCACCTCGCGGCCACATGTATGCCGTGACTGACGTTCGTAACACCCTACGTACTGG
CGCACCAGCCAATGCCGAAGACGGCCCAATGCCAATGGCATGTTGGAGCTGTAAGAGCCCTGATGTACCTCGTCTGATCG
AAGAACAAGGCGAAGAGGGTTACTTCAAAGGCAAGTGGGCTAAAGGTGGTCCAGAAGTCGTTAACACTATCGGTTGTAGC
GACTGCCACGAAAAAGGCACCCCAAAACTACGCATTTCACGTCCATTCGCTGAGCGCGCAATGGAAACTATCGGCACACC
ATTCGACAAAGCTTCGAAGAAAGACAAGCAATCTATGGTTTGCGGTCAGTGCCACGTTGAATACTATTTCGAGAAGAAAG
ACGATCGCAAAGGTTTCGTAAAATTCCCATGGGATTCAGGTACTACTGTTGAACAAATGGAAGCTTACTACGATGCTATC
GAGTTCTCTGACTGGACTCATGCGCTGTCTAAGACACCAATGCTGAAGGCACAACACCCTGGTTATGAAACTTGGAAACT
GGGCGTACACGGTAAGAATGATGTGAGCTGTGTTGACTGTCACATGCCTAAAGTGACTAACGACAAAGGTCGTAAGTACA
CAGATCATAAAGTGGGTAACCCATTCGATCGCTTCGACGAAACCTGTGCAACTTGCCATAGCCAAAGCAAAGAGTTCTTA
GAAGGCATCACTAAAGAGCGTTATGCCAAAGTGAAAGAGCTGAAAGCTCGCGCTGAAGGTCAATTAGTGAAAGCACACTT
CGAAGCCGCTAAAGCGTGGGAAGTGGGTGCCACCGAAGCTGAAATGAAGCCAATCCTGACTGATATCCGTCACGCTCAAT
GGCGCTGGGACTTCGCGATTGCTTCTCACGGTGTTGCGGCTCACGCGCCAGAAGAAGCACTGCGTATCCTAGGTACTGCG
GTTGATAAAGCGGCTGATGCTCGCGTGAAATTAGCCCAATTACTGGCGAAGAAAGGCGTGACTGACGCGGTTGCTATCCC
TGATATCTCTACCAAAGCGAAAGCTCAAGCTGCACTGGGTATGGACATGGACAAGATGAATGCAGAGAAAGAAGCATTCA
AGAAAGACGTGTTACCTAAGTGGGATGAAGAAGCGAAAAAACGCGAAGCAACTTACAAGTAA

Upstream 100 bases:

>100_bases
TCTAGCGCAAACTTCGTTCTAGATACCCCCTAAGAGGTATGTCTTTGGAGGGTTTAAGCCACTAACTTTGGCCTTGGAGA
TGTCGTGATTTATTAAAATA

Downstream 100 bases:

>100_bases
ACATCCTTGAATATGAGCTAACCCCTCATAGTAAAGTCCCCGCAATTTAGGTTGCGGGGGCTTTTTATTTTAACGGTCGA
TTTGTTTGTTCGGGCTTATT

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAKSEEITDALAGDPSLVILWAG
YGFAKDYNAPRGHMYAVTDVRNTLRTGAPANAEDGPMPMACWSCKSPDVPRLIEEQGEEGYFKGKWAKGGPEVVNTIGCS
DCHEKGTPKLRISRPFAERAMETIGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
EFSDWTHALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFDETCATCHSQSKEFL
EGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEAEMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTA
VDKAADARVKLAQLLAKKGVTDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDVLPKWDEEAKKREATYK

Sequences:

>Translated_473_residues
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAKSEEITDALAGDPSLVILWAG
YGFAKDYNAPRGHMYAVTDVRNTLRTGAPANAEDGPMPMACWSCKSPDVPRLIEEQGEEGYFKGKWAKGGPEVVNTIGCS
DCHEKGTPKLRISRPFAERAMETIGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
EFSDWTHALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFDETCATCHSQSKEFL
EGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEAEMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTA
VDKAADARVKLAQLLAKKGVTDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDVLPKWDEEAKKREATYK
>Mature_473_residues
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAKSEEITDALAGDPSLVILWAG
YGFAKDYNAPRGHMYAVTDVRNTLRTGAPANAEDGPMPMACWSCKSPDVPRLIEEQGEEGYFKGKWAKGGPEVVNTIGCS
DCHEKGTPKLRISRPFAERAMETIGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
EFSDWTHALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFDETCATCHSQSKEFL
EGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEAEMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTA
VDKAADARVKLAQLLAKKGVTDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDVLPKWDEEAKKREATYK

Specific function: Plays a role in nitrite reduction

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family

Homologues:

Organism=Escherichia coli, GI1790506, Length=438, Percent_Identity=64.1552511415525, Blast_Score=614, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFA_SHESA (A0KSX7)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_868302.1
- ProteinModelPortal:   A0KSX7
- SMR:   A0KSX7
- STRING:   A0KSX7
- GeneID:   4476868
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_0658
- NMPDR:   fig|94122.5.peg.798
- eggNOG:   COG3303
- HOGENOM:   HBG488281
- OMA:   RDINSAC
- PhylomeDB:   A0KSX7
- ProtClustDB:   PRK11125
- BioCyc:   SSP94122:SHEWANA3_0658-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01182
- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031
- PIRSF:   PIRSF000243
- TIGRFAMs:   TIGR03152

Pfam domain/function: PF02335 Cytochrom_C552

EC number: =1.7.2.2

Molecular weight: Translated: 52673; Mature: 52673

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 121-121 BINDING 124-124 BINDING 159-159 BINDING 162-162 BINDING 201-201 BINDING 204-204 BINDING 208-208 BINDING 259-259 BINDING 277-277 BINDING 280-280 BINDING 309-309 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SEEITDALAGDPSLVILWAGYGFAKDYNAPRGHMYAVTDVRNTLRTGAPANAEDGPMPMA
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEE
CWSCKSPDVPRLIEEQGEEGYFKGKWAKGGPEVVNTIGCSDCHEKGTPKLRISRPFAERA
EECCCCCCCCHHHHHCCCCCCEECCCCCCCHHHHHHCCCCHHHHCCCCCEEEECHHHHHH
METIGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
HHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHH
EFSDWTHALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGN
HHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEECCCCCCC
PFDRFDETCATCHSQSKEFLEGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHH
EMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTAVDKAADARVKLAQLLAKKGV
HHHHHHHHHHHHHEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDVLPKWDEEAKKREATYK
CCEEECCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MQHGDEMMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYNSWHDTAK
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SEEITDALAGDPSLVILWAGYGFAKDYNAPRGHMYAVTDVRNTLRTGAPANAEDGPMPMA
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEE
CWSCKSPDVPRLIEEQGEEGYFKGKWAKGGPEVVNTIGCSDCHEKGTPKLRISRPFAERA
EECCCCCCCCHHHHHCCCCCCEECCCCCCCHHHHHHCCCCHHHHCCCCCEEEECHHHHHH
METIGTPFDKASKKDKQSMVCGQCHVEYYFEKKDDRKGFVKFPWDSGTTVEQMEAYYDAI
HHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHH
EFSDWTHALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGN
HHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEECCCCCCC
PFDRFDETCATCHSQSKEFLEGITKERYAKVKELKARAEGQLVKAHFEAAKAWEVGATEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHH
EMKPILTDIRHAQWRWDFAIASHGVAAHAPEEALRILGTAVDKAADARVKLAQLLAKKGV
HHHHHHHHHHHHHEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDAVAIPDISTKAKAQAALGMDMDKMNAEKEAFKKDVLPKWDEEAKKREATYK
CCEEECCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA