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The map label for this gene is miaA [H]

Identifier: 117919049

GI number: 117919049

Start: 699889

End: 700779

Strand: Direct

Name: miaA [H]

Synonym: Shewana3_0596

Alternate gene names: 117919049

Gene position: 699889-700779 (Clockwise)

Preceding gene: 117919048

Following gene: 117919050

Centisome position: 14.08

GC content: 49.49

Gene sequence:

>891_bases
ATGGGACCAACGGCGTCCGGAAAAACCGCTTTAGCACTCGAATTAGCCGAAAAGCATAATTGCGAGATTATCTCGGTTGA
TTCAGCATTGATCTATCGCGGGATGGACATCGGCAGTGCTAAACCCTCTGCCGAGGAATTAGCGCGAGGTCCACATCGAC
TGATCGACATCCGCGATCCGGCAGAGAGCTACTCCGCCGCCGATTTTAGAGCCGATGCGCTGCGCGAGATTGAACAGATT
ATTAGCATGGGAAAGACCCCCGTATTAGTGGGCGGGACCATGATGTACTTTAAGGCGCTTTTAGAAGGATTATCGCCGCT
ACCGAGTGCCGATGAGGCGATTCGGGCCGAGATCCAAGCCGAAGCGGACGCAAACGGATGGGAAGCACTGCACGAGCAAT
TAAGGGAGATAGATCCCGTATCGGCAGAAAGAATTCATCCTAATGATCCACAAAGGCTTTCAAGAGCGATAGAAGTATAT
AGAATAAGCGGTAAGAGTCTGACCGAGTTAACTCAAACCAAGTCGGCTCCTTTACCCTACGATGTGGTGCAATTTGCCAT
TGCGCCCCGTGAGCGCAAAGTGCTGCATGAGTTAATCGGGCAGCGTTTTCGGATAATGTTAGAGCAAGGTTTTATTGATG
AAGTCGCCCAGCTGAAAGCGAGAGGTGACCTACATTTAGATTTGCCCTCAATGCGTTGCGTGGGATATCGACAATGTTGG
CAGTATCTCGATGGTGAATTTGACTATGATACTATGGTCGAAAAAGCCGTTGCCGCTACTAGGCAATTAGCCAAGCGTCA
ATTAACATGGTTGCGTAGTTGGCCAGAGTTGAATTGGCTTGAAAGTGGTGCTGAGGGGAATTTAGTTACACTTATGCGTC
AATGCCGCTAG

Upstream 100 bases:

>100_bases
AGACAGCAAATTGCCGATAAAGCAGTATCCTTACCTTGGCAATCGTGGCTAGAAGAGCAAGCAAGTGAATAAAGAATTAC
AGCCAAAAGTGGTTTTTTTG

Downstream 100 bases:

>100_bases
CTTATTAGCCCTTGCTGTATAATACTAAAACTAAACAAAAAACTTCACTGATAACGATTTATTTTTATATAAATTAAAAA
GGAAATAATAAAATGGCTAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSAEELARGPHRLIDIRDPAESYSAADFRADALREIEQI
ISMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQAEADANGWEALHEQLREIDPVSAERIHPNDPQRLSRAIEVY
RISGKSLTELTQTKSAPLPYDVVQFAIAPRERKVLHELIGQRFRIMLEQGFIDEVAQLKARGDLHLDLPSMRCVGYRQCW
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR

Sequences:

>Translated_296_residues
MGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSAEELARGPHRLIDIRDPAESYSAADFRADALREIEQI
ISMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQAEADANGWEALHEQLREIDPVSAERIHPNDPQRLSRAIEVY
RISGKSLTELTQTKSAPLPYDVVQFAIAPRERKVLHELIGQRFRIMLEQGFIDEVAQLKARGDLHLDLPSMRCVGYRQCW
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR
>Mature_295_residues
GPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSAEELARGPHRLIDIRDPAESYSAADFRADALREIEQII
SMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQAEADANGWEALHEQLREIDPVSAERIHPNDPQRLSRAIEVYR
ISGKSLTELTQTKSAPLPYDVVQFAIAPRERKVLHELIGQRFRIMLEQGFIDEVAQLKARGDLHLDLPSMRCVGYRQCWQ
YLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=304, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1790613, Length=282, Percent_Identity=67.7304964539007, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25144712, Length=270, Percent_Identity=28.5185185185185, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=282, Percent_Identity=27.3049645390071, Blast_Score=125, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24649830, Length=232, Percent_Identity=34.9137931034483, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33201; Mature: 33070

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSAEELARGPHRLIDIRDP
CCCCCCCCCEEEEEHHHHCCCCEEEECHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCC
AESYSAADFRADALREIEQIISMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EADANGWEALHEQLREIDPVSAERIHPNDPQRLSRAIEVYRISGKSLTELTQTKSAPLPY
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
DVVQFAIAPRERKVLHELIGQRFRIMLEQGFIDEVAQLKARGDLHLDLPSMRCVGYRQCW
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
GPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSAEELARGPHRLIDIRDP
CCCCCCCCEEEEEHHHHCCCCEEEECHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCC
AESYSAADFRADALREIEQIISMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EADANGWEALHEQLREIDPVSAERIHPNDPQRLSRAIEVYRISGKSLTELTQTKSAPLPY
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
DVVQFAIAPRERKVLHELIGQRFRIMLEQGFIDEVAQLKARGDLHLDLPSMRCVGYRQCW
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA