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The map label for this gene is pleD [H]

Identifier: 117918726

GI number: 117918726

Start: 302810

End: 304267

Strand: Direct

Name: pleD [H]

Synonym: Shewana3_0269

Alternate gene names: 117918726

Gene position: 302810-304267 (Clockwise)

Preceding gene: 117918725

Following gene: 117918727

Centisome position: 6.09

GC content: 45.2

Gene sequence:

>1458_bases
ATGGGCAATCACGTTTGGAATGATAAAAAGAAGTTTATTTGGCTACTGTCAGTGCTGTTGCTGGTGGCCTTTTTAGTCAC
CAGTGGTATTAGTTATAAAGTGGCCCACGACTCCTTGAGTCAGCAGATTGAGAGCAATACTTTACCTCTAACCAGCGATA
ATATTTATTCAGAAATTCAACAGGATCTGCTGCGCCCCATCTTTATTTCCTCGCTGATGGCGCAGGATACCTTTGTGCGT
GACTGGATGTTGAATCATGAGCAAGAACCCGAAAAACTGGTCCGTTACCTCAAAGAAATTCAAGAAAAATACGATACAGT
CACCAGCTTTTTTGTCTCTGAGCAGAGTCGCAACTATTACCATTCGACGGGCATATTGAAGAAAATCCAAGATATCGATC
CAAATGATGCCTGGTATTTTCGCGTACGTTCTTTACCCGAGTCTGAGCATTACGAAATCAATATCGATTCAGACACCGCG
GATCGCACTAAGACAACCGTATTTGTTAACTATAAAGTGTTCGATTTTGAAGGTAAGTTTATTGGTGTTACTGGCGTTGG
CCTTGCGGTTGAGAAGGTCAAGGCATTGATTGAACTCTATCAGAAGCGCTACAACCGTCGGGTGTTCTTCACCGATAGAC
AAGGTCGTGTCACGCTCCATGGCGATGAGTATGATGGTGCCGATTCGCTGCAAACCAGCGCTGGGCTTGAGCAACTCGCT
ACGCGGATTTTAACCAGCCCGAGTGCGGCCTTTAGCTACCAACGCAACGGTAAAACCGCCTACTTAAACAGCCGCTTAGT
ACCTGAATTTAAATGGTATCTTATCGTAGAGCAGGAGGATGCGCCGCAGGAGCAAGAGCTGCTCAATACCTTTTGGGGTA
ACTTAGCTTTAAGTTTATTGGTGACGATTGGGATTTTACTGATTTCAAACATGACCTTAGGTCGTTATCAGCGCAAACTT
GAGGTCATGGCTTCAACCGATAAACTCACTGGGGCCGCTAATCGCCAGGTGTTTGAAGAGTATTTTCATCAGGCATTAGA
GAAGGCCAAGCTATCCCAAGCGCCGATTTCCATTCTGCTTGTCGATATCGATCATTTTAAGAAAGTAAACGATAGCTATG
GTCATAGCATTGGTGACCTAGTATTAAAAACAGTGGCTAACCTACTAAGAAGTCAACTAAAAAACCAGGATGTGCTGTGT
CGTTGGGGCGGTGAGGAATTCTTGATATTAGTGCCAGAGCAGGATTTAAGTCAGGCTGCTGAACTGGCAGAACAATTTCG
CGATTTAATCTCCCAACGGGAGCTTAAAGTGAATGGCGTGCACATTGCTATTACTGTGAGTATTGGGGTTGCCGAATACC
AAAAACTCGAGCCTGCGGAAGATTTGATTAAGCGAGCGGATCTCGCCCTGTATCAGGCGAAAGAAGCGGGGCGCAATCAA
GTGGTTCTCAATCAGTAG

Upstream 100 bases:

>100_bases
ATTGTGGAAATATTGTGCGGTCGGCAAGGGATTACGTTAACATTCAGGCAACGCATCTCGTTGAAATAAAATAATAAGCC
TGCCGTTGTTGGAGTTGGAA

Downstream 100 bases:

>100_bases
TATGCTGCTATGATGGCCGAATCTGCCAAGGGGATTGTTTTGCTTAAGGCCGCGTTTGAGTGGTTAAGCCGTTCGGGCAG
AGGTAGGTGACGGATTGCGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWMLNHEQEPEKLVRYLKEIQEKYDTVTSFFVSEQSRNYYHSTGILKKIQDIDPNDAWYFRVRSLPESEHYEINIDSDTA
DRTKTTVFVNYKVFDFEGKFIGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGRVTLHGDEYDGADSLQTSAGLEQLA
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWGNLALSLLVTIGILLISNMTLGRYQRKL
EVMASTDKLTGAANRQVFEEYFHQALEKAKLSQAPISILLVDIDHFKKVNDSYGHSIGDLVLKTVANLLRSQLKNQDVLC
RWGGEEFLILVPEQDLSQAAELAEQFRDLISQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKEAGRNQ
VVLNQ

Sequences:

>Translated_485_residues
MGNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWMLNHEQEPEKLVRYLKEIQEKYDTVTSFFVSEQSRNYYHSTGILKKIQDIDPNDAWYFRVRSLPESEHYEINIDSDTA
DRTKTTVFVNYKVFDFEGKFIGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGRVTLHGDEYDGADSLQTSAGLEQLA
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWGNLALSLLVTIGILLISNMTLGRYQRKL
EVMASTDKLTGAANRQVFEEYFHQALEKAKLSQAPISILLVDIDHFKKVNDSYGHSIGDLVLKTVANLLRSQLKNQDVLC
RWGGEEFLILVPEQDLSQAAELAEQFRDLISQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKEAGRNQ
VVLNQ
>Mature_484_residues
GNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVRD
WMLNHEQEPEKLVRYLKEIQEKYDTVTSFFVSEQSRNYYHSTGILKKIQDIDPNDAWYFRVRSLPESEHYEINIDSDTAD
RTKTTVFVNYKVFDFEGKFIGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGRVTLHGDEYDGADSLQTSAGLEQLAT
RILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWGNLALSLLVTIGILLISNMTLGRYQRKLE
VMASTDKLTGAANRQVFEEYFHQALEKAKLSQAPISILLVDIDHFKKVNDSYGHSIGDLVLKTVANLLRSQLKNQDVLCR
WGGEEFLILVPEQDLSQAAELAEQFRDLISQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKEAGRNQV
VLNQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=41.2790697674419, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87082007, Length=202, Percent_Identity=38.1188118811881, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=37.3493975903614, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=39.7590361445783, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.7088607594937, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=31.3609467455621, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=34.8571428571429, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=32.967032967033, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787802, Length=197, Percent_Identity=29.4416243654822, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=32.0754716981132, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=30.5882352941176, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=152, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=189, Percent_Identity=24.8677248677249, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081921, Length=137, Percent_Identity=28.4671532846715, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=150, Percent_Identity=31.3333333333333, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55508; Mature: 55377

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWMLNHEQEPEKLVRYLKEIQEKYDTVTSFFVSEQSRNYY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
HSTGILKKIQDIDPNDAWYFRVRSLPESEHYEINIDSDTADRTKTTVFVNYKVFDFEGKF
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEEEEEECCCE
IGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGRVTLHGDEYDGADSLQTSAGLEQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHH
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWGNLALSLL
HHHHCCCCHHEEECCCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VTIGILLISNMTLGRYQRKLEVMASTDKLTGAANRQVFEEYFHQALEKAKLSQAPISILL
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VDIDHFKKVNDSYGHSIGDLVLKTVANLLRSQLKNQDVLCRWGGEEFLILVPEQDLSQAA
EEHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCHHHHHHH
ELAEQFRDLISQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKEAGRNQ
HHHHHHHHHHHHCCEEECCEEEEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
VVLNQ
EEECC
>Mature Secondary Structure 
GNHVWNDKKKFIWLLSVLLLVAFLVTSGISYKVAHDSLSQQIESNTLPLTSDNIYSEIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWMLNHEQEPEKLVRYLKEIQEKYDTVTSFFVSEQSRNYY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
HSTGILKKIQDIDPNDAWYFRVRSLPESEHYEINIDSDTADRTKTTVFVNYKVFDFEGKF
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEEEEEECCCE
IGVTGVGLAVEKVKALIELYQKRYNRRVFFTDRQGRVTLHGDEYDGADSLQTSAGLEQLA
EEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHH
TRILTSPSAAFSYQRNGKTAYLNSRLVPEFKWYLIVEQEDAPQEQELLNTFWGNLALSLL
HHHHCCCCHHEEECCCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VTIGILLISNMTLGRYQRKLEVMASTDKLTGAANRQVFEEYFHQALEKAKLSQAPISILL
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VDIDHFKKVNDSYGHSIGDLVLKTVANLLRSQLKNQDVLCRWGGEEFLILVPEQDLSQAA
EEHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCHHHHHHH
ELAEQFRDLISQRELKVNGVHIAITVSIGVAEYQKLEPAEDLIKRADLALYQAKEAGRNQ
HHHHHHHHHHHHCCEEECCEEEEEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
VVLNQ
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA