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The map label for this gene is murI

Identifier: 117918638

GI number: 117918638

Start: 213948

End: 214769

Strand: Direct

Name: murI

Synonym: Shewana3_0180

Alternate gene names: 117918638

Gene position: 213948-214769 (Clockwise)

Preceding gene: 117918636

Following gene: 117918640

Centisome position: 4.3

GC content: 48.54

Gene sequence:

>822_bases
TTGTCGCAACCAATATTAGTATTCGATTCTGGCATCGGCGGTCTATCCGTATTGGCCGAAATTCGCAAACTGCTCCCACA
TCACGATTACTGCTATTTATTCGATAATGCCAGATTGCCCTATGGCGAATTGGAAGAGCAGGAACTTATCTCGGGTTGTG
TTGCGCTCATCGACCAAGTTGTCGAGCGTACCCACGCTGCCATTGTTGTCGTAGCTTGCAATACCGCGAGTACTGTTGTG
CTGCCCGCGCTGCGTGCCACTCTATCTATTCCTGTGGTGGGCGTTGTCCCCGCCATTAAACCCGCGGCGCAGTTATCTAA
AAGTAAGCGTATTGGTTTGCTGGCCACTCCTGGTACGGTCAAGCGTCACTATACCTACGAACTCATTAGCCAATTTGCCG
ATGATTGCCACGTTGAGCTGTTCGGATCTTCCGAATTAGTGCTAATGGCCGAACAGAAGATTGCTACCGGCCAATTAGAT
ATGGTGCGGTTAACACAGGTGCTATCACCAATAGTCGAAGCGGACTTAGATGTTTTGGTGCTTGGATGTACTCACTTCCC
CATGCTGCGTGATGAACTGCAACAGGTATTAGGCCAAGGTGTCACCTTGCTCGATTCTGGAGAGGCTATCGCCAAGCGAG
TCAAAACCTTGTTGGCTGAGACTAAGAGTGATGAGCAAGTTCAGGAAGAAGACGCGCACAGTAATTTACTTATGCAGGCG
TTTTATACTAAAGCAGAAATCAGTGAGGGATTAGCGACCACGTTAGTCGATTGTGGCTTTTCAACCCTAGAGCGAATCAC
CACAATCAACTCAAACGGATAA

Upstream 100 bases:

>100_bases
ACTAGGTGACACGAATGTCCAGCATCTTGGTTTTGCGGTTTGAGCAAAGCTGTGGGAAAATCCGCCCGCGCATGGGGCAA
CGAATTAACTGGAGACGGGC

Downstream 100 bases:

>100_bases
ATTAACGCGGATTATTCATCGGTACCTGTACGGTCGGCAGCTTCCGAATCTTGAGTCTTCGCTTCTCTAACCTTTAAGGT
TCTTTCTTGGAAGTTGAAAT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSQPILVFDSGIGGLSVLAEIRKLLPHHDYCYLFDNARLPYGELEEQELISGCVALIDQVVERTHAAIVVVACNTASTVV
LPALRATLSIPVVGVVPAIKPAAQLSKSKRIGLLATPGTVKRHYTYELISQFADDCHVELFGSSELVLMAEQKIATGQLD
MVRLTQVLSPIVEADLDVLVLGCTHFPMLRDELQQVLGQGVTLLDSGEAIAKRVKTLLAETKSDEQVQEEDAHSNLLMQA
FYTKAEISEGLATTLVDCGFSTLERITTINSNG

Sequences:

>Translated_273_residues
MSQPILVFDSGIGGLSVLAEIRKLLPHHDYCYLFDNARLPYGELEEQELISGCVALIDQVVERTHAAIVVVACNTASTVV
LPALRATLSIPVVGVVPAIKPAAQLSKSKRIGLLATPGTVKRHYTYELISQFADDCHVELFGSSELVLMAEQKIATGQLD
MVRLTQVLSPIVEADLDVLVLGCTHFPMLRDELQQVLGQGVTLLDSGEAIAKRVKTLLAETKSDEQVQEEDAHSNLLMQA
FYTKAEISEGLATTLVDCGFSTLERITTINSNG
>Mature_272_residues
SQPILVFDSGIGGLSVLAEIRKLLPHHDYCYLFDNARLPYGELEEQELISGCVALIDQVVERTHAAIVVVACNTASTVVL
PALRATLSIPVVGVVPAIKPAAQLSKSKRIGLLATPGTVKRHYTYELISQFADDCHVELFGSSELVLMAEQKIATGQLDM
VRLTQVLSPIVEADLDVLVLGCTHFPMLRDELQQVLGQGVTLLDSGEAIAKRVKTLLAETKSDEQVQEEDAHSNLLMQAF
YTKAEISEGLATTLVDCGFSTLERITTINSNG

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=268, Percent_Identity=48.134328358209, Blast_Score=220, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_SHESA (A0KRK5)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_867830.1
- ProteinModelPortal:   A0KRK5
- SMR:   A0KRK5
- STRING:   A0KRK5
- GeneID:   4477943
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_0180
- NMPDR:   fig|94122.5.peg.359
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- PhylomeDB:   A0KRK5
- ProtClustDB:   PRK00865
- BioCyc:   SSP94122:SHEWANA3_0180-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29587; Mature: 29455

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPILVFDSGIGGLSVLAEIRKLLPHHDYCYLFDNARLPYGELEEQELISGCVALIDQV
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VERTHAAIVVVACNTASTVVLPALRATLSIPVVGVVPAIKPAAQLSKSKRIGLLATPGTV
HHHHCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCEEEECCCCH
KRHYTYELISQFADDCHVELFGSSELVLMAEQKIATGQLDMVRLTQVLSPIVEADLDVLV
HHHHHHHHHHHHHCCCEEEEECCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCCEEE
LGCTHFPMLRDELQQVLGQGVTLLDSGEAIAKRVKTLLAETKSDEQVQEEDAHSNLLMQA
ECCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FYTKAEISEGLATTLVDCGFSTLERITTINSNG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQPILVFDSGIGGLSVLAEIRKLLPHHDYCYLFDNARLPYGELEEQELISGCVALIDQV
CCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VERTHAAIVVVACNTASTVVLPALRATLSIPVVGVVPAIKPAAQLSKSKRIGLLATPGTV
HHHHCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCEEEECCCCH
KRHYTYELISQFADDCHVELFGSSELVLMAEQKIATGQLDMVRLTQVLSPIVEADLDVLV
HHHHHHHHHHHHHCCCEEEEECCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCCEEE
LGCTHFPMLRDELQQVLGQGVTLLDSGEAIAKRVKTLLAETKSDEQVQEEDAHSNLLMQA
ECCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FYTKAEISEGLATTLVDCGFSTLERITTINSNG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA