Click here to switch to the map view.

The map label for this gene is nudE [H]

Identifier: 117918627

GI number: 117918627

Start: 202502

End: 203053

Strand: Direct

Name: nudE [H]

Synonym: Shewana3_0169

Alternate gene names: 117918627

Gene position: 202502-203053 (Clockwise)

Preceding gene: 117918626

Following gene: 117918628

Centisome position: 4.07

GC content: 48.01

Gene sequence:

>552_bases
ATGTCGCAGCGGCACCATAAGCCGGAAATCTTACATACCGAAGTTGTCGCTAAGAGCCGCTTGTTTCAAATTGAACAGGT
TCATCTTAAATTCTCCAACGGTGTCGAGCGCCAGTATGAGCGGATGAAGGGGGGTAGCCGAGGCGCTGTGATGGTGGTAC
CCATTCATCAAGGCAACATGCTGTTAGCCCGTGAATATGCCGCTGGAACAGATAATTACGAGCTAGGTTTTCCTAAGGGA
TTAATCGACCCTGGTGAGCAAGCTATCGAGGCGGCCAATCGAGAATTACAGGAAGAGATCGGTTTTGGGGCGCGTAAACT
GACACTGTTGAAAGAACTTAGTCTTGCGCCGGGTTATTTCTCTAGCAAGATGCAAATTTTCATCGCAGAAGATCTATATG
AAAGCCGCCTAGAAGGCGATGAGCCTGAACCCATAGATGTAGTGCCTTGGGCGTTGGCCGAGTGGGAAGCGTTACTCGAT
AATAGCGATTTTTCCGAATCTCGTAGTGTGAGCGCATTGTTTTTAGCGCAGAAACACCTACAACTTAAATAA

Upstream 100 bases:

>100_bases
TGGAATAGCCGCACTAAATTCAGGTTGTAGATTAAAGACAGGATAGTAAGATTGTCACTTATCCTAGTTAGTTTGTTGCT
ATTTTTATCCAGGGGCAGTT

Downstream 100 bases:

>100_bases
TCTTTTTCGTTAAAGCGTTCAGCAACTGAGTGCTTGTAAATCATTCGCGAAGTTTGCGATTGGAGTGGTTATGAAGCCAG
AAGAGTTAATCGACGAGGTG

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSQRHHKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMVVPIHQGNMLLAREYAAGTDNYELGFPKG
LIDPGEQAIEAANRELQEEIGFGARKLTLLKELSLAPGYFSSKMQIFIAEDLYESRLEGDEPEPIDVVPWALAEWEALLD
NSDFSESRSVSALFLAQKHLQLK

Sequences:

>Translated_183_residues
MSQRHHKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMVVPIHQGNMLLAREYAAGTDNYELGFPKG
LIDPGEQAIEAANRELQEEIGFGARKLTLLKELSLAPGYFSSKMQIFIAEDLYESRLEGDEPEPIDVVPWALAEWEALLD
NSDFSESRSVSALFLAQKHLQLK
>Mature_182_residues
SQRHHKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMVVPIHQGNMLLAREYAAGTDNYELGFPKGL
IDPGEQAIEAANRELQEEIGFGARKLTLLKELSLAPGYFSSKMQIFIAEDLYESRLEGDEPEPIDVVPWALAEWEALLDN
SDFSESRSVSALFLAQKHLQLK

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=181, Percent_Identity=60.7734806629834, Blast_Score=226, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 20666; Mature: 20535

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRHHKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMVVPIHQGNM
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCE
LLAREYAAGTDNYELGFPKGLIDPGEQAIEAANRELQEEIGFGARKLTLLKELSLAPGYF
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
SSKMQIFIAEDLYESRLEGDEPEPIDVVPWALAEWEALLDNSDFSESRSVSALFLAQKHL
CCCEEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCHHHHHHHHHHH
QLK
CCC
>Mature Secondary Structure 
SQRHHKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMVVPIHQGNM
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCE
LLAREYAAGTDNYELGFPKGLIDPGEQAIEAANRELQEEIGFGARKLTLLKELSLAPGYF
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
SSKMQIFIAEDLYESRLEGDEPEPIDVVPWALAEWEALLDNSDFSESRSVSALFLAQKHL
CCCEEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCHHHHHHHHHHH
QLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]