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The map label for this gene is oprF [H]

Identifier: 117918603

GI number: 117918603

Start: 167372

End: 168835

Strand: Direct

Name: oprF [H]

Synonym: Shewana3_0145

Alternate gene names: 117918603

Gene position: 167372-168835 (Clockwise)

Preceding gene: 117918601

Following gene: 117918608

Centisome position: 3.37

GC content: 47.4

Gene sequence:

>1464_bases
ATGCGTGTTTCTAAGCGGAGTTATTTAGCGGGGTTATTGGCTATGTCGACGAGCCTAATGCCATTGTCATTAATGGCGGC
GGATGAGGGATTATTTACGCCTTTTCCTACGGCGAAATTAAAGGATGAATTGACGCGCCATTATGCCGAATATCCCTTTA
TTATCAGCGCGGGTGAACAAAGGTCTGACTATCGTTTTAAGCCCATCTTCGGTCAGGTCCATCAGCAAAAATACAAATTA
GCGCCCGAATATAGCCCTGCGCATATTCTCGATAATTACCGTGAGCAGATTAAAAAGCTTGGTGGTGAGGTCCTATTCGA
GTGTCTGCTTGAGGCCTGTGGTGATTCTCGCCTATTGGAAGAGCAAATTGCCCTGTTAGATGGTGTTATTGGTGTTGAAA
GTGGTTACTTGTTTGCTCGAATGAAGAATGAGCAAAAAGAGTTTTATACTGCCATACAAGCCGCAAATCTGAAGAGTTCT
GCGGGTATTCATATTACGACGCTCGAGGTGATCCCCGAGCCGCTAGATTTAGTCACAGTTAATGCTGCATTTTTGCAGCA
GGCGCCTAAGCAAATCGAGTTTAAAGATAATCAAAAGAAGGATACCAACGGCGCCGAGGATCATCCGCTTATCAGCCGCT
TAAAGGGAAGCTATATCCAGGACTTTAAACGCCAAGGCTTTGCGAGCACTGCCTTTGTGTCTTCAATGGATGCAAGCAAT
AAGCCTGTGCTGGTGGAGCGTGCGGGTAAGTTGACTCAAAATGGCTATTCAATACCAAAGGAATATTCGGGTTACGAGGT
GTTCACTAACTACAAGGTGGCATTGACTAAGCTCGGATTCGAGACGCAATTTGAGTGTCAGGGAAAGGCCTGCGGTAGGG
AGGATAGGTTTATCCAAGCCATCGATTCTCTAGTGAACATTGGTAACGAAGACAGCCAGTTTTATGCTTGGTATACCTTA
ACGCGCCCCGAGGGGAATGTGCATGTGAGCACTTATACCCTTGGTTATATCGGCGGGTTATGGACCGACTTGCGGGTGTT
AGAGGAGACTGAGCTTAAGGATGACCGTGTGGGGATCAATCTTGAGGCGCTGACCGACCAAATCGAAGCCAAGGGGCATG
TTGCCTTAGAAGGGCTGTTATTTGAGTACGATAGCGACAAGTTGTTGCCCGAGGCGCAGCCCGTTGTCGAGGTGCTTGCC
AGTTACTTAAAGGCAAATCCTAAGCGTGCATTTTATGTGGTGGGTCATACCGATGACAAAGGTAATCAAGCCTATAACCA
AGGTCTATCCCAACGCCGCGCGGTGGCGATTGTGAAGTTATTGACCACGAGTCATGGTATTCAAACTCAACAATTGGAAG
CCGTAGGCATAGGTGAACATGCTCCCGTTGCTAGTAACAGTGATGAGGCGGGGCAGCAGCAGAATCGCCGTGTCGAGTTA
GTATTGAGATCGGATATGAAGTAA

Upstream 100 bases:

>100_bases
CCTCAATCACGCTAAAGTATTAACTTGTTTCATATTTGTTATTGCGCAATCCGTTTAGTGTTACTTTTCGCAGCAGTATT
TCTATGGAGGGTAAATATTG

Downstream 100 bases:

>100_bases
GCCCAATCGTTTGATTCAATGGATATAAAAATGGCGTCAGCTCTTGTCGAGTTGGCGCCATTTTGTTTATCTGCGATCGC
TCAGCCGACGTCTCTGCCAA

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRVSKRSYLAGLLAMSTSLMPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQRSDYRFKPIFGQVHQQKYKL
APEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLEEQIALLDGVIGVESGYLFARMKNEQKEFYTAIQAANLKSS
AGIHITTLEVIPEPLDLVTVNAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALTKLGFETQFECQGKACGREDRFIQAIDSLVNIGNEDSQFYAWYTL
TRPEGNVHVSTYTLGYIGGLWTDLRVLEETELKDDRVGINLEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLA
SYLKANPKRAFYVVGHTDDKGNQAYNQGLSQRRAVAIVKLLTTSHGIQTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
VLRSDMK

Sequences:

>Translated_487_residues
MRVSKRSYLAGLLAMSTSLMPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQRSDYRFKPIFGQVHQQKYKL
APEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLEEQIALLDGVIGVESGYLFARMKNEQKEFYTAIQAANLKSS
AGIHITTLEVIPEPLDLVTVNAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALTKLGFETQFECQGKACGREDRFIQAIDSLVNIGNEDSQFYAWYTL
TRPEGNVHVSTYTLGYIGGLWTDLRVLEETELKDDRVGINLEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLA
SYLKANPKRAFYVVGHTDDKGNQAYNQGLSQRRAVAIVKLLTTSHGIQTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
VLRSDMK
>Mature_487_residues
MRVSKRSYLAGLLAMSTSLMPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQRSDYRFKPIFGQVHQQKYKL
APEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLEEQIALLDGVIGVESGYLFARMKNEQKEFYTAIQAANLKSS
AGIHITTLEVIPEPLDLVTVNAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALTKLGFETQFECQGKACGREDRFIQAIDSLVNIGNEDSQFYAWYTL
TRPEGNVHVSTYTLGYIGGLWTDLRVLEETELKDDRVGINLEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLA
SYLKANPKRAFYVVGHTDDKGNQAYNQGLSQRRAVAIVKLLTTSHGIQTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
VLRSDMK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=104, Percent_Identity=37.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788957, Length=108, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787191, Length=117, Percent_Identity=37.6068376068376, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 54404; Mature: 54404

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSKRSYLAGLLAMSTSLMPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQ
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
RSDYRFKPIFGQVHQQKYKLAPEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLE
CCCCEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EQIALLDGVIGVESGYLFARMKNEQKEFYTAIQAANLKSSAGIHITTLEVIPEPLDLVTV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEE
NAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
CHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCC
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALTKLGFETQFECQGKACGREDRFIQA
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCEEECCCCCCCCCHHHHHH
IDSLVNIGNEDSQFYAWYTLTRPEGNVHVSTYTLGYIGGLWTDLRVLEETELKDDRVGIN
HHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCC
LEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLASYLKANPKRAFYVVGHTDDK
HHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GNQAYNQGLSQRRAVAIVKLLTTSHGIQTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCHHHHHHCCEEEE
VLRSDMK
EEECCCC
>Mature Secondary Structure
MRVSKRSYLAGLLAMSTSLMPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQ
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
RSDYRFKPIFGQVHQQKYKLAPEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLE
CCCCEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EQIALLDGVIGVESGYLFARMKNEQKEFYTAIQAANLKSSAGIHITTLEVIPEPLDLVTV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEE
NAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
CHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCC
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALTKLGFETQFECQGKACGREDRFIQA
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCEEECCCCCCCCCHHHHHH
IDSLVNIGNEDSQFYAWYTLTRPEGNVHVSTYTLGYIGGLWTDLRVLEETELKDDRVGIN
HHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCC
LEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLASYLKANPKRAFYVVGHTDDK
HHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GNQAYNQGLSQRRAVAIVKLLTTSHGIQTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCHHHHHHCCEEEE
VLRSDMK
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]