Click here to switch to the map view.

The map label for this gene is pdhD [H]

Identifier: 116872449

GI number: 116872449

Start: 1048139

End: 1049542

Strand: Direct

Name: pdhD [H]

Synonym: lwe1031

Alternate gene names: 116872449

Gene position: 1048139-1049542 (Clockwise)

Preceding gene: 116872448

Following gene: 116872451

Centisome position: 37.25

GC content: 41.17

Gene sequence:

>1404_bases
ATGGTAGTAGGCGATTTTCCAGAAGAAAGAGACACCATAGTCATCGGTGCAGGCCCAGGTGGGTATGTAGCGGCAATCCG
AGCTGCACAACTCGGACAAAAAGTTACCATTATTGAAAAAGAATATTACGGCGGTGTGTGCTTAAACGTTGGATGTATTC
CCTCTAAAGCACTTATCACAATCGGTCATCGTTTTAAAGAAGCTGGACACTCTGATAACATGGGTATTACAGCGGATAAC
GTAAACTTAGACTTCACAAAAGCACAAGAATGGAAAGGCGGCGTAGTTAACAAGCTTACATCAGGTGTTAAAGGCCTTCT
TAAGAAAAATAAAGTTGAAATGTTAGAAGGAGAAGCGTTCTTCGTGGATGATCATTCCTTGCGTGTGATTCACCCTGACT
CCGCTCAAACTTATACATTCAACAACGTTATCATTGCAACAGGATCTCGTCCAATCGAAATCCCAGGTTTCAAATATGGT
AAACGTGTATTAAGCTCAACTGGTGCACTTGCACTAACAGAAGTTCCGAAAAAATTAGTCGTAATTGGCGGCGGATATAT
CGGAACAGAACTAGGTGGAGCATTCGCTAACCTTGGTACAGAACTAACTATCCTTGAAGGTGGTCCAGAAATCTTACCAA
CGTATGAAAAAGATATGGTTTCCCTAGTTAAACGTAATCTGAAAAGCAAAAACGTTGAAATGGTTACTAAAGCACTTGCA
AAATCTGCTGAAGAAACTGAAAACGGCGTTAAAGTAACTTATGAAGCTAACGGTGAAACTAAAACTATCGAAGCTGATTA
TGTTTTAGTAACAGTAGGTCGTCGTCCAAATACAGACGAAATCGGTTTAGAACAAGCTGGCGTTAAAGTAACTGAACGTG
GCTTAGTAGAAGTAGACAAACAAGGTCGTTCTAACGTTTCTAACATTTTCGCAATTGGTGATATCGTTCCTGGTGTTCCT
CTAGCTCACAAAGCTAGCTATGAAGCAAAAATCGCTGCTGAAGCAATTGCTGGTGAAAAATCCGAAAACGATTATACAGC
ACTACCAGCTGTTGTTTTCAGTGATCCAGAACTTGCAACAGTCGGCTTAACAGAAAAAGAAGCAAAAGAAAAAGGCTTTG
ATGTAAAAGCTGCTAAATTCCCATTCGGTGGTAACGGTCGTGCACTTTCTTTAGATGCACCAGAAGGATTTGTTCGTCTA
GTTACTCGTAAAGAAGATGGCCTAGTTATCGGTGCGCAAGTTGCCGGAATGAACGCTTCTGATATTATTTCAGAAATCGG
TCTAGCAATCGAATCAGGTATTACAGCTGAAGATATCGCGCTTACTATTCACGCTCACCCATCACTTGGAGAGCTTACAA
TGGAAGCAGCTGAACTAGCTTTAGGTCGCCCAATTCATATGTAA

Upstream 100 bases:

>100_bases
GACCACCGTGTCATTGACGGAGCAACTGCTCAAAAAGCAATGAACAATATTAAACGTTTATTAAACGATCCAGAATTATT
ACTAATGGAGGTGTAACAAA

Downstream 100 bases:

>100_bases
ACAACTTAGAAGCGGAGATATCCTTTATGGGTATTTCCGCTTTTCTATATAGAAATAAGTGGTTGTTTTCACAAAAATAA
TTTTTAGTTCATTTTTTGAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALITIGHRFKEAGHSDNMGITADN
VNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAFFVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYG
KRVLSSTGALALTEVPKKLVVIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDKQGRSNVSNIFAIGDIVPGVP
LAHKASYEAKIAAEAIAGEKSENDYTALPAVVFSDPELATVGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRL
VTRKEDGLVIGAQVAGMNASDIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM

Sequences:

>Translated_467_residues
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALITIGHRFKEAGHSDNMGITADN
VNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAFFVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYG
KRVLSSTGALALTEVPKKLVVIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDKQGRSNVSNIFAIGDIVPGVP
LAHKASYEAKIAAEAIAGEKSENDYTALPAVVFSDPELATVGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRL
VTRKEDGLVIGAQVAGMNASDIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM
>Mature_467_residues
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALITIGHRFKEAGHSDNMGITADN
VNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAFFVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYG
KRVLSSTGALALTEVPKKLVVIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDKQGRSNVSNIFAIGDIVPGVP
LAHKASYEAKIAAEAIAGEKSENDYTALPAVVFSDPELATVGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRL
VTRKEDGLVIGAQVAGMNASDIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=40.3887688984881, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=28.6624203821656, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=29.3478260869565, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=27.0925110132159, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=27.0925110132159, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=27.0925110132159, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=27.0925110132159, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=27.0925110132159, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=26.7543859649123, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=25.4988913525499, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786307, Length=450, Percent_Identity=43.1111111111111, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI87081717, Length=446, Percent_Identity=30.0448430493274, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=30.1149425287356, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=27.3118279569892, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1789065, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=41.2663755458515, Blast_Score=311, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=30.2277432712215, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71983419, Length=434, Percent_Identity=26.9585253456221, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=434, Percent_Identity=26.9585253456221, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=23.5173824130879, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=219, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=37.7637130801688, Blast_Score=292, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=30, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=27.6507276507277, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=42.0168067226891, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=30.3157894736842, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=30.3157894736842, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=30.3157894736842, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=26.6247379454927, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49488; Mature: 49488

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALIT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCEEE
IGHRFKEAGHSDNMGITADNVNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAF
EHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEE
FVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYGKRVLSSTGALALTEVPKKLV
EEECCEEEEECCCCCCEEEECEEEEEECCCEEECCCCHHHHHHHHCCCCEEEECCCCEEE
VIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
EEECCEECHHHCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDK
HHHHHCCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCHHHCCCEEECCCCEEECC
QGRSNVSNIFAIGDIVPGVPLAHKASYEAKIAAEAIAGEKSENDYTALPAVVFSDPELAT
CCCCCHHHEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEECCEEEECCCCEEE
VGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRLVTRKEDGLVIGAQVAGMNAS
ECCCHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCHH
DIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVVGDFPEERDTIVIGAGPGGYVAAIRAAQLGQKVTIIEKEYYGGVCLNVGCIPSKALIT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCEEE
IGHRFKEAGHSDNMGITADNVNLDFTKAQEWKGGVVNKLTSGVKGLLKKNKVEMLEGEAF
EHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEE
FVDDHSLRVIHPDSAQTYTFNNVIIATGSRPIEIPGFKYGKRVLSSTGALALTEVPKKLV
EEECCEEEEECCCCCCEEEECEEEEEECCCEEECCCCHHHHHHHHCCCCEEEECCCCEEE
VIGGGYIGTELGGAFANLGTELTILEGGPEILPTYEKDMVSLVKRNLKSKNVEMVTKALA
EEECCEECHHHCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KSAEETENGVKVTYEANGETKTIEADYVLVTVGRRPNTDEIGLEQAGVKVTERGLVEVDK
HHHHHCCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCHHHCCCEEECCCCEEECC
QGRSNVSNIFAIGDIVPGVPLAHKASYEAKIAAEAIAGEKSENDYTALPAVVFSDPELAT
CCCCCHHHEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEECCEEEECCCCEEE
VGLTEKEAKEKGFDVKAAKFPFGGNGRALSLDAPEGFVRLVTRKEDGLVIGAQVAGMNAS
ECCCHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCHH
DIISEIGLAIESGITAEDIALTIHAHPSLGELTMEAAELALGRPIHM
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]